Adoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses

Despite long-term mass drug administration programmes, approximately 220 million people are still infected with filariae in endemic regions. Several research studies have characterized host immune responses but a major obstacle for research on human filariae has been the inability to obtain adult wo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anna Wiszniewsky, Laura E. Layland, Kathrin Arndts, Lisa M. Wadephul, Ruth S. E. Tamadaho, Dennis Borrero-Wolff, Valerine C. Chunda, Chi Anizette Kien, Achim Hoerauf, Samuel Wanji, Manuel Ritter
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Filariae Litomosoides sigmodontis CD4+ and CD8+ T cells adoptive transfer anti-filarial immunity Th17 polarization Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/dcf93911ea2a4f10bc20e763473f133b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dcf93911ea2a4f10bc20e763473f133b
record_format	dspace
spelling	oai:doaj.org-article:dcf93911ea2a4f10bc20e763473f133b2021-11-18T05:34:57ZAdoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses1664-322410.3389/fimmu.2021.777860https://doaj.org/article/dcf93911ea2a4f10bc20e763473f133b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.777860/fullhttps://doaj.org/toc/1664-3224Despite long-term mass drug administration programmes, approximately 220 million people are still infected with filariae in endemic regions. Several research studies have characterized host immune responses but a major obstacle for research on human filariae has been the inability to obtain adult worms which in turn has hindered analysis on infection kinetics and immune signalling. Although the Litomosoides sigmodontis filarial mouse model is well-established, the complex immunological mechanisms associated with filarial control and disease progression remain unclear and translation to human infections is difficult, especially since human filarial infections in rodents are limited. To overcome these obstacles, we performed adoptive immune cell transfer experiments into RAG2IL-2Rγ-deficient C57BL/6 mice. These mice lack T, B and natural killer cells and are susceptible to infection with the human filaria Loa loa. In this study, we revealed a long-term release of L. sigmodontis offspring (microfilariae) in RAG2IL-2Rγ-deficient C57BL/6 mice, which contrasts to C57BL/6 mice which normally eliminate the parasites before patency. We further showed that CD4+ T cells isolated from acute L. sigmodontis-infected C57BL/6 donor mice or mice that already cleared the infection were able to eliminate the parasite and prevent inflammation at the site of infection. In addition, the clearance of the parasites was associated with Th17 polarization of the CD4+ T cells. Consequently, adoptive transfer of immune cell subsets into RAG2IL-2Rγ-deficient C57BL/6 mice will provide an optimal platform to decipher characteristics of distinct immune cells that are crucial for the immunity against rodent and human filarial infections and moreover, might be useful for preclinical research, especially about the efficacy of macrofilaricidal drugs.Anna WiszniewskyLaura E. LaylandLaura E. LaylandKathrin ArndtsLisa M. WadephulRuth S. E. TamadahoDennis Borrero-WolffValerine C. ChundaValerine C. ChundaChi Anizette KienChi Anizette KienAchim HoeraufAchim HoeraufAchim HoeraufSamuel WanjiSamuel WanjiManuel RitterFrontiers Media S.A.articleFilariaeLitomosoides sigmodontisCD4+ and CD8+ T cellsadoptive transferanti-filarial immunityTh17 polarizationImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Filariae Litomosoides sigmodontis CD4+ and CD8+ T cells adoptive transfer anti-filarial immunity Th17 polarization Immunologic diseases. Allergy RC581-607
spellingShingle	Filariae Litomosoides sigmodontis CD4+ and CD8+ T cells adoptive transfer anti-filarial immunity Th17 polarization Immunologic diseases. Allergy RC581-607 Anna Wiszniewsky Laura E. Layland Laura E. Layland Kathrin Arndts Lisa M. Wadephul Ruth S. E. Tamadaho Dennis Borrero-Wolff Valerine C. Chunda Valerine C. Chunda Chi Anizette Kien Chi Anizette Kien Achim Hoerauf Achim Hoerauf Achim Hoerauf Samuel Wanji Samuel Wanji Manuel Ritter Adoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses
description	Despite long-term mass drug administration programmes, approximately 220 million people are still infected with filariae in endemic regions. Several research studies have characterized host immune responses but a major obstacle for research on human filariae has been the inability to obtain adult worms which in turn has hindered analysis on infection kinetics and immune signalling. Although the Litomosoides sigmodontis filarial mouse model is well-established, the complex immunological mechanisms associated with filarial control and disease progression remain unclear and translation to human infections is difficult, especially since human filarial infections in rodents are limited. To overcome these obstacles, we performed adoptive immune cell transfer experiments into RAG2IL-2Rγ-deficient C57BL/6 mice. These mice lack T, B and natural killer cells and are susceptible to infection with the human filaria Loa loa. In this study, we revealed a long-term release of L. sigmodontis offspring (microfilariae) in RAG2IL-2Rγ-deficient C57BL/6 mice, which contrasts to C57BL/6 mice which normally eliminate the parasites before patency. We further showed that CD4+ T cells isolated from acute L. sigmodontis-infected C57BL/6 donor mice or mice that already cleared the infection were able to eliminate the parasite and prevent inflammation at the site of infection. In addition, the clearance of the parasites was associated with Th17 polarization of the CD4+ T cells. Consequently, adoptive transfer of immune cell subsets into RAG2IL-2Rγ-deficient C57BL/6 mice will provide an optimal platform to decipher characteristics of distinct immune cells that are crucial for the immunity against rodent and human filarial infections and moreover, might be useful for preclinical research, especially about the efficacy of macrofilaricidal drugs.
format	article
author	Anna Wiszniewsky Laura E. Layland Laura E. Layland Kathrin Arndts Lisa M. Wadephul Ruth S. E. Tamadaho Dennis Borrero-Wolff Valerine C. Chunda Valerine C. Chunda Chi Anizette Kien Chi Anizette Kien Achim Hoerauf Achim Hoerauf Achim Hoerauf Samuel Wanji Samuel Wanji Manuel Ritter
author_facet	Anna Wiszniewsky Laura E. Layland Laura E. Layland Kathrin Arndts Lisa M. Wadephul Ruth S. E. Tamadaho Dennis Borrero-Wolff Valerine C. Chunda Valerine C. Chunda Chi Anizette Kien Chi Anizette Kien Achim Hoerauf Achim Hoerauf Achim Hoerauf Samuel Wanji Samuel Wanji Manuel Ritter
author_sort	Anna Wiszniewsky
title	Adoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses
title_short	Adoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses
title_full	Adoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses
title_fullStr	Adoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses
title_full_unstemmed	Adoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses
title_sort	adoptive transfer of immune cells into rag2il-2rγ-deficient mice during litomosoides sigmodontis infection: a novel approach to investigate filarial-specific immune responses
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/dcf93911ea2a4f10bc20e763473f133b
work_keys_str_mv	AT annawiszniewsky adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT lauraelayland adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT lauraelayland adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT kathrinarndts adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT lisamwadephul adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT ruthsetamadaho adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT dennisborrerowolff adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT valerinecchunda adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT valerinecchunda adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT chianizettekien adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT chianizettekien adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT achimhoerauf adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT achimhoerauf adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT achimhoerauf adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT samuelwanji adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT samuelwanji adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses AT manuelritter adoptivetransferofimmunecellsintorag2il2rgdeficientmiceduringlitomosoidessigmodontisinfectionanovelapproachtoinvestigatefilarialspecificimmuneresponses
_version_	1718424944679845888

Adoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses

Ejemplares similares