Viral loads in nasopharyngeal aspirates and tracheal aspirates among children hospitalized with invasive ventilation for human adenovirus pneumonia

Abstract Purpose To evaluate viral loads in children with human adenovirus (HAdV) pneumonia at different stages of disease and compare the viral load between upper and lower respiratory tract samples. Methods We prospectively enrolled children who required invasive ventilation for HAdV pneumonia. Na...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Le-Yun Xie, Sai-Zhen Zeng, Tian Yu, Xian Hu, Tao Wang, Le Yang, Li-Li Zhong, Jin-Song Li, Zhao-Jun Duan, Bing Zhang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Human adenovirus Mechanical ventilation Nasopharyngeal aspirate Tracheal aspirate Viral load Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/dd090757b6de42cfac78db1ed6792119
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dd090757b6de42cfac78db1ed6792119
record_format	dspace
spelling	oai:doaj.org-article:dd090757b6de42cfac78db1ed67921192021-12-05T12:03:04ZViral loads in nasopharyngeal aspirates and tracheal aspirates among children hospitalized with invasive ventilation for human adenovirus pneumonia10.1186/s12985-021-01711-z1743-422Xhttps://doaj.org/article/dd090757b6de42cfac78db1ed67921192021-11-01T00:00:00Zhttps://doi.org/10.1186/s12985-021-01711-zhttps://doaj.org/toc/1743-422XAbstract Purpose To evaluate viral loads in children with human adenovirus (HAdV) pneumonia at different stages of disease and compare the viral load between upper and lower respiratory tract samples. Methods We prospectively enrolled children who required invasive ventilation for HAdV pneumonia. Nasopharyngeal aspirate (NPA) and tracheal aspirate (TA) samples were collected throughout the entire period of invasive ventilation. Viral detection and quantification were performed using quantitative real-time polymerase chain reaction. Results Ninety-four children were enrolled. The median age of the children was 12.0 months (IQR: 11.0–24.0), and > ninety percent of patients were aged between 6 and 59 months. Seven hundred and nine paired NPA-TA samples were collected. The median viral loads of the NPA and TA samples were 7.31 log10 and 7.50 log10 copies/mL, respectively. Viral loads generally decreased steadily over time. The median viral load after 1, 2, 3, and > 3 weeks of the disease course was 8.65, 7.70, 6.69, and 5.09 log10 copies/mL, respectively, in NPA samples and 8.67, 7.79, 7.08, and 5.53 log10 copies/mL, respectively, in TA samples. Viral load showed a significant negative correlation with time since symptom onset in both NPA samples (Spearman r = − 0.607, P = 0.000) and TA samples (Spearman r = − 0.544, P = 0.000). The predicted duration of HAdV shedding was 60.17 days in the NPA group and 65.81 days in the TA group. Viral loads in NPA and TA from the same subjects correlated well with each other (R2 = 0.694). HAdV loads in NPA and TA were most comparable during the early phase of infection (95% limits of agreement, − 1.36 to 1.30 log10 copies/mL, R2 = 0.746). Variation increased during the late phase of infection (i.e., in follow-up samples), with viral loads remaining significantly higher in TA than NPA. Conclusions In children with HAdV pneumonia, viral loads in both NPA and TA steadily decreased during the course of the disease, and the predicted duration of viral shedding was more than 2 months. The HAdV DNA load of NPA is highly correlated with that of TA, especially in the initial phase of infection.Le-Yun XieSai-Zhen ZengTian YuXian HuTao WangLe YangLi-Li ZhongJin-Song LiZhao-Jun DuanBing ZhangBMCarticleHuman adenovirusMechanical ventilationNasopharyngeal aspirateTracheal aspirateViral loadInfectious and parasitic diseasesRC109-216ENVirology Journal, Vol 18, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Human adenovirus Mechanical ventilation Nasopharyngeal aspirate Tracheal aspirate Viral load Infectious and parasitic diseases RC109-216
spellingShingle	Human adenovirus Mechanical ventilation Nasopharyngeal aspirate Tracheal aspirate Viral load Infectious and parasitic diseases RC109-216 Le-Yun Xie Sai-Zhen Zeng Tian Yu Xian Hu Tao Wang Le Yang Li-Li Zhong Jin-Song Li Zhao-Jun Duan Bing Zhang Viral loads in nasopharyngeal aspirates and tracheal aspirates among children hospitalized with invasive ventilation for human adenovirus pneumonia
description	Abstract Purpose To evaluate viral loads in children with human adenovirus (HAdV) pneumonia at different stages of disease and compare the viral load between upper and lower respiratory tract samples. Methods We prospectively enrolled children who required invasive ventilation for HAdV pneumonia. Nasopharyngeal aspirate (NPA) and tracheal aspirate (TA) samples were collected throughout the entire period of invasive ventilation. Viral detection and quantification were performed using quantitative real-time polymerase chain reaction. Results Ninety-four children were enrolled. The median age of the children was 12.0 months (IQR: 11.0–24.0), and > ninety percent of patients were aged between 6 and 59 months. Seven hundred and nine paired NPA-TA samples were collected. The median viral loads of the NPA and TA samples were 7.31 log10 and 7.50 log10 copies/mL, respectively. Viral loads generally decreased steadily over time. The median viral load after 1, 2, 3, and > 3 weeks of the disease course was 8.65, 7.70, 6.69, and 5.09 log10 copies/mL, respectively, in NPA samples and 8.67, 7.79, 7.08, and 5.53 log10 copies/mL, respectively, in TA samples. Viral load showed a significant negative correlation with time since symptom onset in both NPA samples (Spearman r = − 0.607, P = 0.000) and TA samples (Spearman r = − 0.544, P = 0.000). The predicted duration of HAdV shedding was 60.17 days in the NPA group and 65.81 days in the TA group. Viral loads in NPA and TA from the same subjects correlated well with each other (R2 = 0.694). HAdV loads in NPA and TA were most comparable during the early phase of infection (95% limits of agreement, − 1.36 to 1.30 log10 copies/mL, R2 = 0.746). Variation increased during the late phase of infection (i.e., in follow-up samples), with viral loads remaining significantly higher in TA than NPA. Conclusions In children with HAdV pneumonia, viral loads in both NPA and TA steadily decreased during the course of the disease, and the predicted duration of viral shedding was more than 2 months. The HAdV DNA load of NPA is highly correlated with that of TA, especially in the initial phase of infection.
format	article
author	Le-Yun Xie Sai-Zhen Zeng Tian Yu Xian Hu Tao Wang Le Yang Li-Li Zhong Jin-Song Li Zhao-Jun Duan Bing Zhang
author_facet	Le-Yun Xie Sai-Zhen Zeng Tian Yu Xian Hu Tao Wang Le Yang Li-Li Zhong Jin-Song Li Zhao-Jun Duan Bing Zhang
author_sort	Le-Yun Xie
title	Viral loads in nasopharyngeal aspirates and tracheal aspirates among children hospitalized with invasive ventilation for human adenovirus pneumonia
title_short	Viral loads in nasopharyngeal aspirates and tracheal aspirates among children hospitalized with invasive ventilation for human adenovirus pneumonia
title_full	Viral loads in nasopharyngeal aspirates and tracheal aspirates among children hospitalized with invasive ventilation for human adenovirus pneumonia
title_fullStr	Viral loads in nasopharyngeal aspirates and tracheal aspirates among children hospitalized with invasive ventilation for human adenovirus pneumonia
title_full_unstemmed	Viral loads in nasopharyngeal aspirates and tracheal aspirates among children hospitalized with invasive ventilation for human adenovirus pneumonia
title_sort	viral loads in nasopharyngeal aspirates and tracheal aspirates among children hospitalized with invasive ventilation for human adenovirus pneumonia
publisher	BMC
publishDate	2021
url	https://doaj.org/article/dd090757b6de42cfac78db1ed6792119
work_keys_str_mv	AT leyunxie viralloadsinnasopharyngealaspiratesandtrachealaspiratesamongchildrenhospitalizedwithinvasiveventilationforhumanadenoviruspneumonia AT saizhenzeng viralloadsinnasopharyngealaspiratesandtrachealaspiratesamongchildrenhospitalizedwithinvasiveventilationforhumanadenoviruspneumonia AT tianyu viralloadsinnasopharyngealaspiratesandtrachealaspiratesamongchildrenhospitalizedwithinvasiveventilationforhumanadenoviruspneumonia AT xianhu viralloadsinnasopharyngealaspiratesandtrachealaspiratesamongchildrenhospitalizedwithinvasiveventilationforhumanadenoviruspneumonia AT taowang viralloadsinnasopharyngealaspiratesandtrachealaspiratesamongchildrenhospitalizedwithinvasiveventilationforhumanadenoviruspneumonia AT leyang viralloadsinnasopharyngealaspiratesandtrachealaspiratesamongchildrenhospitalizedwithinvasiveventilationforhumanadenoviruspneumonia AT lilizhong viralloadsinnasopharyngealaspiratesandtrachealaspiratesamongchildrenhospitalizedwithinvasiveventilationforhumanadenoviruspneumonia AT jinsongli viralloadsinnasopharyngealaspiratesandtrachealaspiratesamongchildrenhospitalizedwithinvasiveventilationforhumanadenoviruspneumonia AT zhaojunduan viralloadsinnasopharyngealaspiratesandtrachealaspiratesamongchildrenhospitalizedwithinvasiveventilationforhumanadenoviruspneumonia AT bingzhang viralloadsinnasopharyngealaspiratesandtrachealaspiratesamongchildrenhospitalizedwithinvasiveventilationforhumanadenoviruspneumonia
_version_	1718372348289089536

Viral loads in nasopharyngeal aspirates and tracheal aspirates among children hospitalized with invasive ventilation for human adenovirus pneumonia

Ejemplares similares