Stability of the Virome in Lab- and Field-Collected <named-content content-type="genus-species">Aedes albopictus</named-content> Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of <italic toggle="yes">Aedes</italic> Mosquitoes

ABSTRACT Aedes mosquitoes can efficiently transmit many pathogenic arboviruses, placing a great burden on public health worldwide. In addition, they also carry a number of insect-specific viruses (ISVs), and it was recently suggested that some of these ISVs might form a stable species-specific “core...

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Autores principales:	Chenyan Shi, Lu Zhao, Evans Atoni, Weifeng Zeng, Xiaomin Hu, Jelle Matthijnssens, Zhiming Yuan, Han Xia
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Aedes mosquito Guadeloupe mosquito virus Phasi Charoen-like phasivirus core virome environment-derived core virome vertically transmitted core virome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dd10417ea24a4f53975f7b30e3186d3f
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spelling	oai:doaj.org-article:dd10417ea24a4f53975f7b30e3186d3f2021-12-02T19:46:19ZStability of the Virome in Lab- and Field-Collected <named-content content-type="genus-species">Aedes albopictus</named-content> Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of <italic toggle="yes">Aedes</italic> Mosquitoes10.1128/mSystems.00640-202379-5077https://doaj.org/article/dd10417ea24a4f53975f7b30e3186d3f2020-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00640-20https://doaj.org/toc/2379-5077ABSTRACT Aedes mosquitoes can efficiently transmit many pathogenic arboviruses, placing a great burden on public health worldwide. In addition, they also carry a number of insect-specific viruses (ISVs), and it was recently suggested that some of these ISVs might form a stable species-specific “core virome” in mosquito populations. However, little is known about such a core virome in laboratory colonies and if it is present across different developmental stages. In this study, we compared the viromes in eggs, larvae, pupae, and adults of Aedes albopictus mosquitoes collected from a lab colony and compared each to the virome of different developmental stages collected in the field. The virome in lab-derived A. albopictus was very stable across all stages, consistent with a vertical transmission route of these viruses, and formed a possible “vertically transmitted core virome.” The different stages of field-collected A. albopictus mosquitoes also contained this stable vertically transmitted core virome, as well as another set of viruses (e.g., viruses distantly related to Guadeloupe mosquito virus, Hubei virga-like virus 2, and Sarawak virus) shared by mosquitoes across different stages, which might represent an “environment-derived core virome.” To further study this core set of ISVs, we screened 48 publicly available SRA viral metagenomic data sets of mosquitoes belonging to the genus Aedes, showing that some of the identified ISVs were identified in the majority of SRAs and providing further evidence supporting the core-virome concept. IMPORTANCE Our study revealed that the virome was very stable across all developmental stages of both lab-derived and field-collected Aedes albopictus. The data representing the core virome in lab A. albopictus proved the vertical transmission route of these viruses, forming a “vertically transmitted core virome.” Field mosquitoes also contained this stable vertically transmitted core virome as well as additional viruses, which probably represented “environment-derived core virome” and which therefore were less stable over time and geography. By further screening publicly available SRA viral metagenomic data sets from mosquitoes belonging to the genus Aedes, some of the identified core ISVs were shown to be present in the majority of SRAs, such as Phasi Charoen-like phasivirus and Guadeloupe mosquito virus. How these core ISVs influence the biology of the mosquito host and arbovirus infection and evolution deserves to be further explored.Chenyan ShiLu ZhaoEvans AtoniWeifeng ZengXiaomin HuJelle MatthijnssensZhiming YuanHan XiaAmerican Society for MicrobiologyarticleAedes mosquitoGuadeloupe mosquito virusPhasi Charoen-like phasiviruscore viromeenvironment-derived core viromevertically transmitted core viromeMicrobiologyQR1-502ENmSystems, Vol 5, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aedes mosquito Guadeloupe mosquito virus Phasi Charoen-like phasivirus core virome environment-derived core virome vertically transmitted core virome Microbiology QR1-502
spellingShingle	Aedes mosquito Guadeloupe mosquito virus Phasi Charoen-like phasivirus core virome environment-derived core virome vertically transmitted core virome Microbiology QR1-502 Chenyan Shi Lu Zhao Evans Atoni Weifeng Zeng Xiaomin Hu Jelle Matthijnssens Zhiming Yuan Han Xia Stability of the Virome in Lab- and Field-Collected <named-content content-type="genus-species">Aedes albopictus</named-content> Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of <italic toggle="yes">Aedes</italic> Mosquitoes
description	ABSTRACT Aedes mosquitoes can efficiently transmit many pathogenic arboviruses, placing a great burden on public health worldwide. In addition, they also carry a number of insect-specific viruses (ISVs), and it was recently suggested that some of these ISVs might form a stable species-specific “core virome” in mosquito populations. However, little is known about such a core virome in laboratory colonies and if it is present across different developmental stages. In this study, we compared the viromes in eggs, larvae, pupae, and adults of Aedes albopictus mosquitoes collected from a lab colony and compared each to the virome of different developmental stages collected in the field. The virome in lab-derived A. albopictus was very stable across all stages, consistent with a vertical transmission route of these viruses, and formed a possible “vertically transmitted core virome.” The different stages of field-collected A. albopictus mosquitoes also contained this stable vertically transmitted core virome, as well as another set of viruses (e.g., viruses distantly related to Guadeloupe mosquito virus, Hubei virga-like virus 2, and Sarawak virus) shared by mosquitoes across different stages, which might represent an “environment-derived core virome.” To further study this core set of ISVs, we screened 48 publicly available SRA viral metagenomic data sets of mosquitoes belonging to the genus Aedes, showing that some of the identified ISVs were identified in the majority of SRAs and providing further evidence supporting the core-virome concept. IMPORTANCE Our study revealed that the virome was very stable across all developmental stages of both lab-derived and field-collected Aedes albopictus. The data representing the core virome in lab A. albopictus proved the vertical transmission route of these viruses, forming a “vertically transmitted core virome.” Field mosquitoes also contained this stable vertically transmitted core virome as well as additional viruses, which probably represented “environment-derived core virome” and which therefore were less stable over time and geography. By further screening publicly available SRA viral metagenomic data sets from mosquitoes belonging to the genus Aedes, some of the identified core ISVs were shown to be present in the majority of SRAs, such as Phasi Charoen-like phasivirus and Guadeloupe mosquito virus. How these core ISVs influence the biology of the mosquito host and arbovirus infection and evolution deserves to be further explored.
format	article
author	Chenyan Shi Lu Zhao Evans Atoni Weifeng Zeng Xiaomin Hu Jelle Matthijnssens Zhiming Yuan Han Xia
author_facet	Chenyan Shi Lu Zhao Evans Atoni Weifeng Zeng Xiaomin Hu Jelle Matthijnssens Zhiming Yuan Han Xia
author_sort	Chenyan Shi
title	Stability of the Virome in Lab- and Field-Collected <named-content content-type="genus-species">Aedes albopictus</named-content> Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of <italic toggle="yes">Aedes</italic> Mosquitoes
title_short	Stability of the Virome in Lab- and Field-Collected <named-content content-type="genus-species">Aedes albopictus</named-content> Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of <italic toggle="yes">Aedes</italic> Mosquitoes
title_full	Stability of the Virome in Lab- and Field-Collected <named-content content-type="genus-species">Aedes albopictus</named-content> Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of <italic toggle="yes">Aedes</italic> Mosquitoes
title_fullStr	Stability of the Virome in Lab- and Field-Collected <named-content content-type="genus-species">Aedes albopictus</named-content> Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of <italic toggle="yes">Aedes</italic> Mosquitoes
title_full_unstemmed	Stability of the Virome in Lab- and Field-Collected <named-content content-type="genus-species">Aedes albopictus</named-content> Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of <italic toggle="yes">Aedes</italic> Mosquitoes
title_sort	stability of the virome in lab- and field-collected <named-content content-type="genus-species">aedes albopictus</named-content> mosquitoes across different developmental stages and possible core viruses in the publicly available virome data of <italic toggle="yes">aedes</italic> mosquitoes
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/dd10417ea24a4f53975f7b30e3186d3f
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Stability of the Virome in Lab- and Field-Collected <named-content content-type="genus-species">Aedes albopictus</named-content> Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of <italic toggle="yes">Aedes</italic> Mosquitoes

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