Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study

Abstract Clonal clusters and gene repertoires of Staphylococcus aureus are essential to understand disease and are well characterized in industrialized countries but poorly analysed in developing regions. The objective of this study was to compare the molecular-epidemiologic profiles of S. aureus is...

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Autores principales:	Ulla Ruffing, Abraham Alabi, Theckla Kazimoto, Delfino C. Vubil, Ruslan Akulenko, Salim Abdulla, Pedro Alonso, Markus Bischoff, Anja Germann, Martin P. Grobusch, Volkhard Helms, Jonas Hoffmann, Winfried V. Kern, Peter G. Kremsner, Inacio Mandomando, Alexander Mellmann, Georg Peters, Frieder Schaumburg, Sabine Schubert, Lena Strauß, Marcel Tanner, Hagen von Briesen, Laura Wende, Lutz von Müller, Mathias Herrmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd1244f49781473e9388cf190b2a7c1a
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spelling	oai:doaj.org-article:dd1244f49781473e9388cf190b2a7c1a2021-12-02T11:52:20ZCommunity-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study10.1038/s41598-017-00214-82045-2322https://doaj.org/article/dd1244f49781473e9388cf190b2a7c1a2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00214-8https://doaj.org/toc/2045-2322Abstract Clonal clusters and gene repertoires of Staphylococcus aureus are essential to understand disease and are well characterized in industrialized countries but poorly analysed in developing regions. The objective of this study was to compare the molecular-epidemiologic profiles of S. aureus isolates from Sub-Saharan Africa and Germany. S. aureus isolates from 600 staphylococcal carriers and 600 patients with community-associated staphylococcal disease were characterized by DNA hybridization, clonal complex (CC) attribution, and principal component (PCA)-based gene repertoire analysis. 73% of all CCs identified representing 77% of the isolates contained in these CCs were predominant in either African or German region. Significant differences between African versus German isolates were found for alleles encoding the accessory gene regulator type, enterotoxins, the Panton-Valentine leukocidin, immune evasion gene cluster, and adhesins. PCA in conjunction with silhouette analysis distinguished nine separable PCA clusters, with five clusters primarily comprising of African and two clusters of German isolates. Significant differences between S. aureus lineages in Africa and Germany may be a clue to explain the apparent difference in disease between tropical/(so-called) developing and temperate/industrialized regions. In low-resource countries further clinical-epidemiologic research is warranted not only for neglected tropical diseases but also for major bacterial infections.Ulla RuffingAbraham AlabiTheckla KazimotoDelfino C. VubilRuslan AkulenkoSalim AbdullaPedro AlonsoMarkus BischoffAnja GermannMartin P. GrobuschVolkhard HelmsJonas HoffmannWinfried V. KernPeter G. KremsnerInacio MandomandoAlexander MellmannGeorg PetersFrieder SchaumburgSabine SchubertLena StraußMarcel TannerHagen von BriesenLaura WendeLutz von MüllerMathias HerrmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ulla Ruffing Abraham Alabi Theckla Kazimoto Delfino C. Vubil Ruslan Akulenko Salim Abdulla Pedro Alonso Markus Bischoff Anja Germann Martin P. Grobusch Volkhard Helms Jonas Hoffmann Winfried V. Kern Peter G. Kremsner Inacio Mandomando Alexander Mellmann Georg Peters Frieder Schaumburg Sabine Schubert Lena Strauß Marcel Tanner Hagen von Briesen Laura Wende Lutz von Müller Mathias Herrmann Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study
description	Abstract Clonal clusters and gene repertoires of Staphylococcus aureus are essential to understand disease and are well characterized in industrialized countries but poorly analysed in developing regions. The objective of this study was to compare the molecular-epidemiologic profiles of S. aureus isolates from Sub-Saharan Africa and Germany. S. aureus isolates from 600 staphylococcal carriers and 600 patients with community-associated staphylococcal disease were characterized by DNA hybridization, clonal complex (CC) attribution, and principal component (PCA)-based gene repertoire analysis. 73% of all CCs identified representing 77% of the isolates contained in these CCs were predominant in either African or German region. Significant differences between African versus German isolates were found for alleles encoding the accessory gene regulator type, enterotoxins, the Panton-Valentine leukocidin, immune evasion gene cluster, and adhesins. PCA in conjunction with silhouette analysis distinguished nine separable PCA clusters, with five clusters primarily comprising of African and two clusters of German isolates. Significant differences between S. aureus lineages in Africa and Germany may be a clue to explain the apparent difference in disease between tropical/(so-called) developing and temperate/industrialized regions. In low-resource countries further clinical-epidemiologic research is warranted not only for neglected tropical diseases but also for major bacterial infections.
format	article
author	Ulla Ruffing Abraham Alabi Theckla Kazimoto Delfino C. Vubil Ruslan Akulenko Salim Abdulla Pedro Alonso Markus Bischoff Anja Germann Martin P. Grobusch Volkhard Helms Jonas Hoffmann Winfried V. Kern Peter G. Kremsner Inacio Mandomando Alexander Mellmann Georg Peters Frieder Schaumburg Sabine Schubert Lena Strauß Marcel Tanner Hagen von Briesen Laura Wende Lutz von Müller Mathias Herrmann
author_facet	Ulla Ruffing Abraham Alabi Theckla Kazimoto Delfino C. Vubil Ruslan Akulenko Salim Abdulla Pedro Alonso Markus Bischoff Anja Germann Martin P. Grobusch Volkhard Helms Jonas Hoffmann Winfried V. Kern Peter G. Kremsner Inacio Mandomando Alexander Mellmann Georg Peters Frieder Schaumburg Sabine Schubert Lena Strauß Marcel Tanner Hagen von Briesen Laura Wende Lutz von Müller Mathias Herrmann
author_sort	Ulla Ruffing
title	Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study
title_short	Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study
title_full	Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study
title_fullStr	Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study
title_full_unstemmed	Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study
title_sort	community-associated staphylococcus aureus from sub-saharan africa and germany: a cross-sectional geographic correlation study
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/dd1244f49781473e9388cf190b2a7c1a
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Community-Associated Staphylococcus aureus from Sub-Saharan Africa and Germany: A Cross-Sectional Geographic Correlation Study

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