Impact of technical sources of variation on the hand microbiome dynamics of healthcare workers.

We assessed the dynamics of hand microbial community structure of 34 healthcare workers from a single surgical intensive care unit over a short (3 week) time period, whilst taking into account the technical sources of variability introduced by specimen collection, DNA extraction, and sequencing. Sam...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mariana Rosenthal, Allison E Aiello, Carol Chenoweth, Deborah Goldberg, Elaine Larson, Gregory Gloor, Betsy Foxman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd17d1226b7c4cfe855c454e34cdfc97
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dd17d1226b7c4cfe855c454e34cdfc97
record_format	dspace
spelling	oai:doaj.org-article:dd17d1226b7c4cfe855c454e34cdfc972021-11-18T08:32:24ZImpact of technical sources of variation on the hand microbiome dynamics of healthcare workers.1932-620310.1371/journal.pone.0088999https://doaj.org/article/dd17d1226b7c4cfe855c454e34cdfc972014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24551205/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We assessed the dynamics of hand microbial community structure of 34 healthcare workers from a single surgical intensive care unit over a short (3 week) time period, whilst taking into account the technical sources of variability introduced by specimen collection, DNA extraction, and sequencing. Sample collection took place at 3 different time points. Only the sampling collection method appeared to have a significant impact on the observed hand microbial community structure among the healthcare workers. Analysis of samples collected using glove-juice showed a slightly more similar microbial composition within individual hand samples over time than between the hands of different individuals over time. This was not true for samples collected using a swab, where samples from a single individual were no more similar to each other over time than those among other individuals over time, suggesting they were essentially independent. DNA extraction techniques (lysozyme only versus enzyme cocktail) and sequencing (replicate set 1 versus 2) using Ion Torrent Personal Genome Machine, were not influential to the microbial community structures. Glove-juice sample collection may likely be the method of choice in hand hygiene studies in the healthcare setting.Mariana RosenthalAllison E AielloCarol ChenowethDeborah GoldbergElaine LarsonGregory GloorBetsy FoxmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88999 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariana Rosenthal Allison E Aiello Carol Chenoweth Deborah Goldberg Elaine Larson Gregory Gloor Betsy Foxman Impact of technical sources of variation on the hand microbiome dynamics of healthcare workers.
description	We assessed the dynamics of hand microbial community structure of 34 healthcare workers from a single surgical intensive care unit over a short (3 week) time period, whilst taking into account the technical sources of variability introduced by specimen collection, DNA extraction, and sequencing. Sample collection took place at 3 different time points. Only the sampling collection method appeared to have a significant impact on the observed hand microbial community structure among the healthcare workers. Analysis of samples collected using glove-juice showed a slightly more similar microbial composition within individual hand samples over time than between the hands of different individuals over time. This was not true for samples collected using a swab, where samples from a single individual were no more similar to each other over time than those among other individuals over time, suggesting they were essentially independent. DNA extraction techniques (lysozyme only versus enzyme cocktail) and sequencing (replicate set 1 versus 2) using Ion Torrent Personal Genome Machine, were not influential to the microbial community structures. Glove-juice sample collection may likely be the method of choice in hand hygiene studies in the healthcare setting.
format	article
author	Mariana Rosenthal Allison E Aiello Carol Chenoweth Deborah Goldberg Elaine Larson Gregory Gloor Betsy Foxman
author_facet	Mariana Rosenthal Allison E Aiello Carol Chenoweth Deborah Goldberg Elaine Larson Gregory Gloor Betsy Foxman
author_sort	Mariana Rosenthal
title	Impact of technical sources of variation on the hand microbiome dynamics of healthcare workers.
title_short	Impact of technical sources of variation on the hand microbiome dynamics of healthcare workers.
title_full	Impact of technical sources of variation on the hand microbiome dynamics of healthcare workers.
title_fullStr	Impact of technical sources of variation on the hand microbiome dynamics of healthcare workers.
title_full_unstemmed	Impact of technical sources of variation on the hand microbiome dynamics of healthcare workers.
title_sort	impact of technical sources of variation on the hand microbiome dynamics of healthcare workers.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/dd17d1226b7c4cfe855c454e34cdfc97
work_keys_str_mv	AT marianarosenthal impactoftechnicalsourcesofvariationonthehandmicrobiomedynamicsofhealthcareworkers AT allisoneaiello impactoftechnicalsourcesofvariationonthehandmicrobiomedynamicsofhealthcareworkers AT carolchenoweth impactoftechnicalsourcesofvariationonthehandmicrobiomedynamicsofhealthcareworkers AT deborahgoldberg impactoftechnicalsourcesofvariationonthehandmicrobiomedynamicsofhealthcareworkers AT elainelarson impactoftechnicalsourcesofvariationonthehandmicrobiomedynamicsofhealthcareworkers AT gregorygloor impactoftechnicalsourcesofvariationonthehandmicrobiomedynamicsofhealthcareworkers AT betsyfoxman impactoftechnicalsourcesofvariationonthehandmicrobiomedynamicsofhealthcareworkers
_version_	1718421712785113088

Impact of technical sources of variation on the hand microbiome dynamics of healthcare workers.

Ejemplares similares