The Notch system during pubertal development of the bovine mammary gland

Abstract The development of the mammary gland of cows during pre-weaning and puberty will condition its future productive capacity and warrants special study. In this respect, Notch signaling regulates tissue development and fate by modifying cell proliferation and differentiation and has been invol...

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Autores principales:	Nadia Bonadeo, Damasia Becu-Villalobos, Carolina Cristina, Isabel M. Lacau-Mengido
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd21b1edc4dc435d93971b0637691690
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spelling	oai:doaj.org-article:dd21b1edc4dc435d93971b06376916902021-12-02T15:09:31ZThe Notch system during pubertal development of the bovine mammary gland10.1038/s41598-019-45406-62045-2322https://doaj.org/article/dd21b1edc4dc435d93971b06376916902019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45406-6https://doaj.org/toc/2045-2322Abstract The development of the mammary gland of cows during pre-weaning and puberty will condition its future productive capacity and warrants special study. In this respect, Notch signaling regulates tissue development and fate by modifying cell proliferation and differentiation and has been involved in stem cell maintenance, but has not been extensively studied in the developing mammary glands in cows. We therefore investigated Notch receptor expression and localization, as well as the expression of Notch ligands and target genes in the mammary gland of Holstein heifers in pre- and post-pubertal stages. Notch receptors 1 to 4 were detected by immunohistochemistry in the parenchyma and stroma of the developing gland. The subcellular localization of the four receptors was predominantly cytoplasmic except for NOTCH4, which was mostly nuclear. The membrane and the active intracellular domains of NOTCH paralogues were identified by western blot. NOTCH1 and NOTCH2 active domains increased during pubertal stages while NOTCH3 and NOTCH4 active domains decreased, suggesting strikingly different involvement of NOTCH paralogues in bovine mammary gland development and differentiation. The mRNA expression levels of the target genes HEY1 and HEY2 increased during peri-puberty whereas no variation of HES1 mRNA levels was observed. The mRNA levels of the Notch ligands JAGGED1 and DELTA1 also increased gradually during development. In conclusion, Notch signaling system dynamically varies throughout the development of the mammary gland during puberty pointing to specific time involvement of each component.Nadia BonadeoDamasia Becu-VillalobosCarolina CristinaIsabel M. Lacau-MengidoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nadia Bonadeo Damasia Becu-Villalobos Carolina Cristina Isabel M. Lacau-Mengido The Notch system during pubertal development of the bovine mammary gland
description	Abstract The development of the mammary gland of cows during pre-weaning and puberty will condition its future productive capacity and warrants special study. In this respect, Notch signaling regulates tissue development and fate by modifying cell proliferation and differentiation and has been involved in stem cell maintenance, but has not been extensively studied in the developing mammary glands in cows. We therefore investigated Notch receptor expression and localization, as well as the expression of Notch ligands and target genes in the mammary gland of Holstein heifers in pre- and post-pubertal stages. Notch receptors 1 to 4 were detected by immunohistochemistry in the parenchyma and stroma of the developing gland. The subcellular localization of the four receptors was predominantly cytoplasmic except for NOTCH4, which was mostly nuclear. The membrane and the active intracellular domains of NOTCH paralogues were identified by western blot. NOTCH1 and NOTCH2 active domains increased during pubertal stages while NOTCH3 and NOTCH4 active domains decreased, suggesting strikingly different involvement of NOTCH paralogues in bovine mammary gland development and differentiation. The mRNA expression levels of the target genes HEY1 and HEY2 increased during peri-puberty whereas no variation of HES1 mRNA levels was observed. The mRNA levels of the Notch ligands JAGGED1 and DELTA1 also increased gradually during development. In conclusion, Notch signaling system dynamically varies throughout the development of the mammary gland during puberty pointing to specific time involvement of each component.
format	article
author	Nadia Bonadeo Damasia Becu-Villalobos Carolina Cristina Isabel M. Lacau-Mengido
author_facet	Nadia Bonadeo Damasia Becu-Villalobos Carolina Cristina Isabel M. Lacau-Mengido
author_sort	Nadia Bonadeo
title	The Notch system during pubertal development of the bovine mammary gland
title_short	The Notch system during pubertal development of the bovine mammary gland
title_full	The Notch system during pubertal development of the bovine mammary gland
title_fullStr	The Notch system during pubertal development of the bovine mammary gland
title_full_unstemmed	The Notch system during pubertal development of the bovine mammary gland
title_sort	notch system during pubertal development of the bovine mammary gland
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/dd21b1edc4dc435d93971b0637691690
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The Notch system during pubertal development of the bovine mammary gland

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