Cardiac Cell Patterning on Customized Microelectrode Arrays for Electrophysiological Recordings

Cardiomyocytes (CMs) and fibroblast cells are two essential elements for cardiac tissue structure and function. The interactions between them can alter cardiac electrophysiology and thus contribute to cardiac diseases, such as arrhythmogenesis. One possible explanation is that fibroblasts can direct...

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Autores principales:	Jiaying Ji, Xiang Ren, Pinar Zorlutuna
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microelectrode array cardiomyocytes human pluripotent stem cells micro-patterning Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/dd269cc0826c4af7b7611e1b68d77e8d
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spelling	oai:doaj.org-article:dd269cc0826c4af7b7611e1b68d77e8d2021-11-25T18:23:20ZCardiac Cell Patterning on Customized Microelectrode Arrays for Electrophysiological Recordings10.3390/mi121113512072-666Xhttps://doaj.org/article/dd269cc0826c4af7b7611e1b68d77e8d2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1351https://doaj.org/toc/2072-666XCardiomyocytes (CMs) and fibroblast cells are two essential elements for cardiac tissue structure and function. The interactions between them can alter cardiac electrophysiology and thus contribute to cardiac diseases, such as arrhythmogenesis. One possible explanation is that fibroblasts can directly affect cardiac electrophysiology through electrical coupling with CMs. Therefore, detecting the electrical activities in the CM-fibroblast network is vital for understanding the coupling dynamics among them. Current commercialized platforms for studying cardiac electrophysiology utilize planar microelectrode arrays (MEAs) to record the extracellular field potential (FP) in real-time, but the prearranged electrode configuration highly limits the measurement capabilities at specific locations. Here, we report a custom-designed MEA device with a novel micropatterning method to construct a controlled network of neonatal rat CMs (rCMs) and fibroblast connections for monitoring the electrical activity of rCM-fibroblast co-cultures in a spatially controlled fashion. For the micropatterning of the co-culture, surface topographical features and mobile blockers were used to control the initial attachment locations of a mixture of rCMs and fibroblasts, to form separate beating rCM-fibroblast clusters while leaving empty space for fibroblast growth to connect these clusters. Once the blockers are removed, the proliferating fibroblasts connect and couple the separate beating clusters. Using this method, electrical activity of both rCMs and human-induced-pluripotent-stem-cell-derived cardiomyocytes (iCMs) was examined. The coupling dynamics were studied through the extracellular FP and impedance profile recorded from the MEA device, indicating that the fibroblast bridge provided an RC-type coupling of physically separate rCM-containing clusters and enabled synchronization of these clusters.Jiaying JiXiang RenPinar ZorlutunaMDPI AGarticlemicroelectrode arraycardiomyocyteshuman pluripotent stem cellsmicro-patterningMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1351, p 1351 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microelectrode array cardiomyocytes human pluripotent stem cells micro-patterning Mechanical engineering and machinery TJ1-1570
spellingShingle	microelectrode array cardiomyocytes human pluripotent stem cells micro-patterning Mechanical engineering and machinery TJ1-1570 Jiaying Ji Xiang Ren Pinar Zorlutuna Cardiac Cell Patterning on Customized Microelectrode Arrays for Electrophysiological Recordings
description	Cardiomyocytes (CMs) and fibroblast cells are two essential elements for cardiac tissue structure and function. The interactions between them can alter cardiac electrophysiology and thus contribute to cardiac diseases, such as arrhythmogenesis. One possible explanation is that fibroblasts can directly affect cardiac electrophysiology through electrical coupling with CMs. Therefore, detecting the electrical activities in the CM-fibroblast network is vital for understanding the coupling dynamics among them. Current commercialized platforms for studying cardiac electrophysiology utilize planar microelectrode arrays (MEAs) to record the extracellular field potential (FP) in real-time, but the prearranged electrode configuration highly limits the measurement capabilities at specific locations. Here, we report a custom-designed MEA device with a novel micropatterning method to construct a controlled network of neonatal rat CMs (rCMs) and fibroblast connections for monitoring the electrical activity of rCM-fibroblast co-cultures in a spatially controlled fashion. For the micropatterning of the co-culture, surface topographical features and mobile blockers were used to control the initial attachment locations of a mixture of rCMs and fibroblasts, to form separate beating rCM-fibroblast clusters while leaving empty space for fibroblast growth to connect these clusters. Once the blockers are removed, the proliferating fibroblasts connect and couple the separate beating clusters. Using this method, electrical activity of both rCMs and human-induced-pluripotent-stem-cell-derived cardiomyocytes (iCMs) was examined. The coupling dynamics were studied through the extracellular FP and impedance profile recorded from the MEA device, indicating that the fibroblast bridge provided an RC-type coupling of physically separate rCM-containing clusters and enabled synchronization of these clusters.
format	article
author	Jiaying Ji Xiang Ren Pinar Zorlutuna
author_facet	Jiaying Ji Xiang Ren Pinar Zorlutuna
author_sort	Jiaying Ji
title	Cardiac Cell Patterning on Customized Microelectrode Arrays for Electrophysiological Recordings
title_short	Cardiac Cell Patterning on Customized Microelectrode Arrays for Electrophysiological Recordings
title_full	Cardiac Cell Patterning on Customized Microelectrode Arrays for Electrophysiological Recordings
title_fullStr	Cardiac Cell Patterning on Customized Microelectrode Arrays for Electrophysiological Recordings
title_full_unstemmed	Cardiac Cell Patterning on Customized Microelectrode Arrays for Electrophysiological Recordings
title_sort	cardiac cell patterning on customized microelectrode arrays for electrophysiological recordings
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dd269cc0826c4af7b7611e1b68d77e8d
work_keys_str_mv	AT jiayingji cardiaccellpatterningoncustomizedmicroelectrodearraysforelectrophysiologicalrecordings AT xiangren cardiaccellpatterningoncustomizedmicroelectrodearraysforelectrophysiologicalrecordings AT pinarzorlutuna cardiaccellpatterningoncustomizedmicroelectrodearraysforelectrophysiologicalrecordings
_version_	1718411289689063424

Cardiac Cell Patterning on Customized Microelectrode Arrays for Electrophysiological Recordings

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