Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice

Abstract Metabolomic and transcriptomic approaches were used to dissect the enhanced disease resistance in the plants harbouring a RNA interfering construct of OsWRKY62 and OsWRKY76 (dsOW62/76) genes. The primary metabolic pathways were activated in dsOW62/76 compared with wild-type (ZH17) plants, r...

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Autores principales:	Xiaoxing Liang, Xujun Chen, Cheng Li, Jun Fan, Zejian Guo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd26a61b5feb4820b236e098f1708d7c
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spelling	oai:doaj.org-article:dd26a61b5feb4820b236e098f1708d7c2021-12-02T11:53:05ZMetabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice10.1038/s41598-017-02643-x2045-2322https://doaj.org/article/dd26a61b5feb4820b236e098f1708d7c2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02643-xhttps://doaj.org/toc/2045-2322Abstract Metabolomic and transcriptomic approaches were used to dissect the enhanced disease resistance in the plants harbouring a RNA interfering construct of OsWRKY62 and OsWRKY76 (dsOW62/76) genes. The primary metabolic pathways were activated in dsOW62/76 compared with wild-type (ZH17) plants, revealed by increased accumulation of amino acids and constituents of citric acid cycle etc. Contents of phenolic acids derived from phenylpropanoid pathway were elevated in dsOW62/76 plants. Importantly, phenolamides, conjugates of the phenolic acids with amines, were detected in large number and mostly at higher levels in dsOW62/76 compared with ZH17 plants; however, the free pools of flavonoids were mostly decreased in dsOW62/76. Salicylic acid (SA) and jasmonic acid (JA)/JA-Ile contents were increased in dsOW62/76 and knockout lines of individual OsWRKY62 and OsWRKY76 genes. Transcription of isochorismate synthase (OsICS1) gene was suppressed in dsOW62/76 and in MeJA-treated rice plants, whereas the transcription level of cinnamoyl-CoA hydratase-dehydrogenase (OsCHD) gene for β-oxidation in peroxisome was increased. The calli with OsCHD mutation showed markedly decreased SA accumulation. These results indicate that OsWRKY62 and OsWRKY76 function as negative regulators of biosynthetic defense-related metabolites and provide evidence for an important role of phenylpropanoid pathway in SA production in rice.Xiaoxing LiangXujun ChenCheng LiJun FanZejian GuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoxing Liang Xujun Chen Cheng Li Jun Fan Zejian Guo Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice
description	Abstract Metabolomic and transcriptomic approaches were used to dissect the enhanced disease resistance in the plants harbouring a RNA interfering construct of OsWRKY62 and OsWRKY76 (dsOW62/76) genes. The primary metabolic pathways were activated in dsOW62/76 compared with wild-type (ZH17) plants, revealed by increased accumulation of amino acids and constituents of citric acid cycle etc. Contents of phenolic acids derived from phenylpropanoid pathway were elevated in dsOW62/76 plants. Importantly, phenolamides, conjugates of the phenolic acids with amines, were detected in large number and mostly at higher levels in dsOW62/76 compared with ZH17 plants; however, the free pools of flavonoids were mostly decreased in dsOW62/76. Salicylic acid (SA) and jasmonic acid (JA)/JA-Ile contents were increased in dsOW62/76 and knockout lines of individual OsWRKY62 and OsWRKY76 genes. Transcription of isochorismate synthase (OsICS1) gene was suppressed in dsOW62/76 and in MeJA-treated rice plants, whereas the transcription level of cinnamoyl-CoA hydratase-dehydrogenase (OsCHD) gene for β-oxidation in peroxisome was increased. The calli with OsCHD mutation showed markedly decreased SA accumulation. These results indicate that OsWRKY62 and OsWRKY76 function as negative regulators of biosynthetic defense-related metabolites and provide evidence for an important role of phenylpropanoid pathway in SA production in rice.
format	article
author	Xiaoxing Liang Xujun Chen Cheng Li Jun Fan Zejian Guo
author_facet	Xiaoxing Liang Xujun Chen Cheng Li Jun Fan Zejian Guo
author_sort	Xiaoxing Liang
title	Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice
title_short	Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice
title_full	Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice
title_fullStr	Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice
title_full_unstemmed	Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice
title_sort	metabolic and transcriptional alternations for defense by interfering oswrky62 and oswrky76 transcriptions in rice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/dd26a61b5feb4820b236e098f1708d7c
work_keys_str_mv	AT xiaoxingliang metabolicandtranscriptionalalternationsfordefensebyinterferingoswrky62andoswrky76transcriptionsinrice AT xujunchen metabolicandtranscriptionalalternationsfordefensebyinterferingoswrky62andoswrky76transcriptionsinrice AT chengli metabolicandtranscriptionalalternationsfordefensebyinterferingoswrky62andoswrky76transcriptionsinrice AT junfan metabolicandtranscriptionalalternationsfordefensebyinterferingoswrky62andoswrky76transcriptionsinrice AT zejianguo metabolicandtranscriptionalalternationsfordefensebyinterferingoswrky62andoswrky76transcriptionsinrice
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Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice

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