The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering

Marketa Bacakova,1,2 Jana Musilkova,1 Tomas Riedel,3 Denisa Stranska,4 Eduard Brynda,3 Margit Zaloudkova,5 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Second Faculty of Medicine, Charles University in Prague, 3Institute of...

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Autores principales:	Bacakova M, Musilkova J, Riedel T, Stranska D, Brynda E, Zaloudkova M, Bacakova L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	nanofibers skin tissue engineering fibroblasts fibrin collagen ascorbic acid Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dd26faeb06eb4ae3aa36ef1a7897b7ea
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spelling	oai:doaj.org-article:dd26faeb06eb4ae3aa36ef1a7897b7ea2021-12-02T00:40:17ZThe potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering1178-2013https://doaj.org/article/dd26faeb06eb4ae3aa36ef1a7897b7ea2016-02-01T00:00:00Zhttps://www.dovepress.com/the-potential-applications-of-fibrin-coated-electrospun-polylactide-na-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Marketa Bacakova,1,2 Jana Musilkova,1 Tomas Riedel,3 Denisa Stranska,4 Eduard Brynda,3 Margit Zaloudkova,5 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Second Faculty of Medicine, Charles University in Prague, 3Institute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, 4InStar Technologies, Liberec, 5Institute of Rock Structure and Mechanics, Czech Academy of Sciences, Prague, Czech Republic Abstract: Fibrin plays an important role during wound healing and skin regeneration. It is often applied in clinical practice for treatment of skin injuries or as a component of skin substitutes. We prepared electrospun nanofibrous membranes made from poly(L-lactide) modified with a thin fibrin nanocoating. Fibrin surrounded the individual fibers in the membrane and also formed a thin fibrous mesh on several places on the membrane surface. The cell-free fibrin nanocoating remained stable in the cell culture medium for 14 days and did not change its morphology. On membranes populated with human dermal fibroblasts, the rate of fibrin degradation correlated with the degree of cell proliferation. The cell spreading, mitochondrial activity, and cell population density were significantly higher on membranes coated with fibrin than on nonmodified membranes, and this cell performance was further improved by the addition of ascorbic acid in the cell culture medium. Similarly, fibrin stimulated the expression and synthesis of collagen I in human dermal fibroblasts, and this effect was further enhanced by ascorbic acid. The expression of beta1-integrins was also improved by fibrin, and on pure polylactide membranes, it was slightly enhanced by ascorbic acid. In addition, ascorbic acid promoted deposition of collagen I in the form of a fibrous extracellular matrix. Thus, the combination of nanofibrous membranes with a fibrin nanocoating and ascorbic acid seems to be particularly advantageous for skin tissue engineering. Keywords: electrospun nanofibers, nanocoating, skin tissue engineering, fibroblasts, fibrin, ascorbic acid, nanotechnology, nanomedicine, collagen I synthesis, beta1-integrinsBacakova MMusilkova JRiedel TStranska DBrynda EZaloudkova MBacakova LDove Medical Pressarticlenanofibersskin tissue engineeringfibroblastsfibrincollagenascorbic acidMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 771-789 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanofibers skin tissue engineering fibroblasts fibrin collagen ascorbic acid Medicine (General) R5-920
spellingShingle	nanofibers skin tissue engineering fibroblasts fibrin collagen ascorbic acid Medicine (General) R5-920 Bacakova M Musilkova J Riedel T Stranska D Brynda E Zaloudkova M Bacakova L The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering
description	Marketa Bacakova,1,2 Jana Musilkova,1 Tomas Riedel,3 Denisa Stranska,4 Eduard Brynda,3 Margit Zaloudkova,5 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Second Faculty of Medicine, Charles University in Prague, 3Institute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, 4InStar Technologies, Liberec, 5Institute of Rock Structure and Mechanics, Czech Academy of Sciences, Prague, Czech Republic Abstract: Fibrin plays an important role during wound healing and skin regeneration. It is often applied in clinical practice for treatment of skin injuries or as a component of skin substitutes. We prepared electrospun nanofibrous membranes made from poly(L-lactide) modified with a thin fibrin nanocoating. Fibrin surrounded the individual fibers in the membrane and also formed a thin fibrous mesh on several places on the membrane surface. The cell-free fibrin nanocoating remained stable in the cell culture medium for 14 days and did not change its morphology. On membranes populated with human dermal fibroblasts, the rate of fibrin degradation correlated with the degree of cell proliferation. The cell spreading, mitochondrial activity, and cell population density were significantly higher on membranes coated with fibrin than on nonmodified membranes, and this cell performance was further improved by the addition of ascorbic acid in the cell culture medium. Similarly, fibrin stimulated the expression and synthesis of collagen I in human dermal fibroblasts, and this effect was further enhanced by ascorbic acid. The expression of beta1-integrins was also improved by fibrin, and on pure polylactide membranes, it was slightly enhanced by ascorbic acid. In addition, ascorbic acid promoted deposition of collagen I in the form of a fibrous extracellular matrix. Thus, the combination of nanofibrous membranes with a fibrin nanocoating and ascorbic acid seems to be particularly advantageous for skin tissue engineering. Keywords: electrospun nanofibers, nanocoating, skin tissue engineering, fibroblasts, fibrin, ascorbic acid, nanotechnology, nanomedicine, collagen I synthesis, beta1-integrins
format	article
author	Bacakova M Musilkova J Riedel T Stranska D Brynda E Zaloudkova M Bacakova L
author_facet	Bacakova M Musilkova J Riedel T Stranska D Brynda E Zaloudkova M Bacakova L
author_sort	Bacakova M
title	The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering
title_short	The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering
title_full	The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering
title_fullStr	The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering
title_full_unstemmed	The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering
title_sort	potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/dd26faeb06eb4ae3aa36ef1a7897b7ea
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The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering

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