RANGING OF ANTIPHOSPOLIPID ANTIBODIES IN THE PATIENTS WITH THROMBOPHILIA AND RECURRENT MISCARRIAGE

Laboratory diagnosis of antiphospholipid syndrome (APS) is based on detection of antiphospholipid antibodies (aPLs). E.g., aPLs are directed against conformational epitopes of the so-called “co-factor” proteins: β2-gycoprotein 1 (β2-GP1), annexin V (An V) and prothrombin (Pt) that are formed during...

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Autores principales:	O. Yu. Tkachenko, S. V Lapin, A. A. Shmonin, L. N. Solovyova, E. A. Bondareva, S. A. Selkov, S. V. Chepanov, Areg A. Totolian, Dirk Roggenbuck
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	antiphospholipid syndrome antiphospholipid antibodies enzyme-linked immunosorbent assays multi-line dot assay new methods Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/dd2883eb948d44c78372e9204484807f
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spelling	oai:doaj.org-article:dd2883eb948d44c78372e9204484807f2021-11-18T08:03:47ZRANGING OF ANTIPHOSPOLIPID ANTIBODIES IN THE PATIENTS WITH THROMBOPHILIA AND RECURRENT MISCARRIAGE1563-06252313-741X10.15789/1563-0625-2018-5-753-762https://doaj.org/article/dd2883eb948d44c78372e9204484807f2018-11-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1644https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XLaboratory diagnosis of antiphospholipid syndrome (APS) is based on detection of antiphospholipid antibodies (aPLs). E.g., aPLs are directed against conformational epitopes of the so-called “co-factor” proteins: β2-gycoprotein 1 (β2-GP1), annexin V (An V) and prothrombin (Pt) that are formed during interaction with phospholipids – cardiolipin (CL), phosphatic acid (Pha), phosphatidylcholine (Pch), phosphatidylethanolamine (Pe), phosphatidylglycerol (Pg), phosphatidylinositol (Pi), phosphatidylserine (Ps). A routine methodology of detection based on ELISA testing is challenged by new tests when the antigen is absorbed on another kind of support like microbeads or membranes that can influence density of conformational epitopes for aPL’s binding. The aim of our study was to compare the results of aPLs detection by ELISA and multi-line immunodot assay (MLD). We collected blood serum samples from 45 patients with noncardioembolic ischemic strokes, 19 patients with recurrent deep vein thrombosis of lower limbs, 44 females with recurrent miscarriages, and 50 clinically healthy donors. To compare the results of aPL detection by ELISA and MLD kits, the test systems from different manufacturers were evaluated. We used an ELISA kits for detection of antibodies to CL IgG, aCL IgM, β2-GP1 produced by Euroimmun AG (Mr1) and Orgentec Diagnostica GmbH (Mr2) and MLD – for detection of antibodies to CL, β2-GP1, Pch, Pe, Pg, Pi, Ps, AnV and Pt (Medipan GmbH, Mr3). When a cut-off titer was used as the main index, 30.5% of patients were aPLs-positive with ELISA method by Mr1 and 38%, wiht Mr2. By MLD aPls were detected in 30% of patients. In the same cohort, medium and high aPLs titers (> 40 U/mL) were determined in 12% of patients using ELISA kits. Positive and highly positive aPLs titers were determined in 16% when using a new method by Mr3. Medium and high titer were detected only for antibodies to β2-GP1, CL, An V, Pha and Phs. The use of ELISA approach for detection of aPLs in patients with thrombosis and obstetric pathology is associated with relatively high number of low-positive ELISA results. Due to higher sensitivity for medium and high aPLs titers, MLD testing may be used as a confirming method for APS diagnosis.O. Yu. TkachenkoS. V LapinA. A. ShmoninL. N. SolovyovaE. A. BondarevaS. A. SelkovS. V. ChepanovAreg A. TotolianDirk RoggenbuckSPb RAACIarticleantiphospholipid syndromeantiphospholipid antibodiesenzyme-linked immunosorbent assaysmulti-line dot assaynew methodsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 5, Pp 753-762 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	antiphospholipid syndrome antiphospholipid antibodies enzyme-linked immunosorbent assays multi-line dot assay new methods Immunologic diseases. Allergy RC581-607
spellingShingle	antiphospholipid syndrome antiphospholipid antibodies enzyme-linked immunosorbent assays multi-line dot assay new methods Immunologic diseases. Allergy RC581-607 O. Yu. Tkachenko S. V Lapin A. A. Shmonin L. N. Solovyova E. A. Bondareva S. A. Selkov S. V. Chepanov Areg A. Totolian Dirk Roggenbuck RANGING OF ANTIPHOSPOLIPID ANTIBODIES IN THE PATIENTS WITH THROMBOPHILIA AND RECURRENT MISCARRIAGE
description	Laboratory diagnosis of antiphospholipid syndrome (APS) is based on detection of antiphospholipid antibodies (aPLs). E.g., aPLs are directed against conformational epitopes of the so-called “co-factor” proteins: β2-gycoprotein 1 (β2-GP1), annexin V (An V) and prothrombin (Pt) that are formed during interaction with phospholipids – cardiolipin (CL), phosphatic acid (Pha), phosphatidylcholine (Pch), phosphatidylethanolamine (Pe), phosphatidylglycerol (Pg), phosphatidylinositol (Pi), phosphatidylserine (Ps). A routine methodology of detection based on ELISA testing is challenged by new tests when the antigen is absorbed on another kind of support like microbeads or membranes that can influence density of conformational epitopes for aPL’s binding. The aim of our study was to compare the results of aPLs detection by ELISA and multi-line immunodot assay (MLD). We collected blood serum samples from 45 patients with noncardioembolic ischemic strokes, 19 patients with recurrent deep vein thrombosis of lower limbs, 44 females with recurrent miscarriages, and 50 clinically healthy donors. To compare the results of aPL detection by ELISA and MLD kits, the test systems from different manufacturers were evaluated. We used an ELISA kits for detection of antibodies to CL IgG, aCL IgM, β2-GP1 produced by Euroimmun AG (Mr1) and Orgentec Diagnostica GmbH (Mr2) and MLD – for detection of antibodies to CL, β2-GP1, Pch, Pe, Pg, Pi, Ps, AnV and Pt (Medipan GmbH, Mr3). When a cut-off titer was used as the main index, 30.5% of patients were aPLs-positive with ELISA method by Mr1 and 38%, wiht Mr2. By MLD aPls were detected in 30% of patients. In the same cohort, medium and high aPLs titers (> 40 U/mL) were determined in 12% of patients using ELISA kits. Positive and highly positive aPLs titers were determined in 16% when using a new method by Mr3. Medium and high titer were detected only for antibodies to β2-GP1, CL, An V, Pha and Phs. The use of ELISA approach for detection of aPLs in patients with thrombosis and obstetric pathology is associated with relatively high number of low-positive ELISA results. Due to higher sensitivity for medium and high aPLs titers, MLD testing may be used as a confirming method for APS diagnosis.
format	article
author	O. Yu. Tkachenko S. V Lapin A. A. Shmonin L. N. Solovyova E. A. Bondareva S. A. Selkov S. V. Chepanov Areg A. Totolian Dirk Roggenbuck
author_facet	O. Yu. Tkachenko S. V Lapin A. A. Shmonin L. N. Solovyova E. A. Bondareva S. A. Selkov S. V. Chepanov Areg A. Totolian Dirk Roggenbuck
author_sort	O. Yu. Tkachenko
title	RANGING OF ANTIPHOSPOLIPID ANTIBODIES IN THE PATIENTS WITH THROMBOPHILIA AND RECURRENT MISCARRIAGE
title_short	RANGING OF ANTIPHOSPOLIPID ANTIBODIES IN THE PATIENTS WITH THROMBOPHILIA AND RECURRENT MISCARRIAGE
title_full	RANGING OF ANTIPHOSPOLIPID ANTIBODIES IN THE PATIENTS WITH THROMBOPHILIA AND RECURRENT MISCARRIAGE
title_fullStr	RANGING OF ANTIPHOSPOLIPID ANTIBODIES IN THE PATIENTS WITH THROMBOPHILIA AND RECURRENT MISCARRIAGE
title_full_unstemmed	RANGING OF ANTIPHOSPOLIPID ANTIBODIES IN THE PATIENTS WITH THROMBOPHILIA AND RECURRENT MISCARRIAGE
title_sort	ranging of antiphospolipid antibodies in the patients with thrombophilia and recurrent miscarriage
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/dd2883eb948d44c78372e9204484807f
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RANGING OF ANTIPHOSPOLIPID ANTIBODIES IN THE PATIENTS WITH THROMBOPHILIA AND RECURRENT MISCARRIAGE

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