How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps

ABSTRACT Bacterial multidrug resistance (MDR) efflux pumps are an important mechanism of antibiotic resistance and are required for many pathogens to cause infection. They are also being harnessed to improve microbial biotechnological processes, including biofuel production. Therefore, scientists of...

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Autores principales:	Jessica M. A. Blair, Laura J. V. Piddock
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dd2a958aa61148d7ae1ba078c53bdbc7
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spelling	oai:doaj.org-article:dd2a958aa61148d7ae1ba078c53bdbc72021-11-15T15:50:19ZHow to Measure Export via Bacterial Multidrug Resistance Efflux Pumps10.1128/mBio.00840-162150-7511https://doaj.org/article/dd2a958aa61148d7ae1ba078c53bdbc72016-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00840-16https://doaj.org/toc/2150-7511ABSTRACT Bacterial multidrug resistance (MDR) efflux pumps are an important mechanism of antibiotic resistance and are required for many pathogens to cause infection. They are also being harnessed to improve microbial biotechnological processes, including biofuel production. Therefore, scientists of many specialties must be able to accurately measure efflux activity. However, myriad methodologies have been described and the most appropriate method is not always clear. Within the scientific literature, many methods are misused or data arising are misinterpreted. The methods for measuring efflux activity can be split into two groups, (i) those that directly measure efflux and (ii) those that measure the intracellular accumulation of a substrate, which is then used to infer efflux activity. Here, we review the methods for measuring efflux and explore the most recent advances in this field, including single-cell or cell-free technologies and mass spectrometry, that are being used to provide more detailed information about efflux pump activity.Jessica M. A. BlairLaura J. V. PiddockAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 4 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Jessica M. A. Blair Laura J. V. Piddock How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps
description	ABSTRACT Bacterial multidrug resistance (MDR) efflux pumps are an important mechanism of antibiotic resistance and are required for many pathogens to cause infection. They are also being harnessed to improve microbial biotechnological processes, including biofuel production. Therefore, scientists of many specialties must be able to accurately measure efflux activity. However, myriad methodologies have been described and the most appropriate method is not always clear. Within the scientific literature, many methods are misused or data arising are misinterpreted. The methods for measuring efflux activity can be split into two groups, (i) those that directly measure efflux and (ii) those that measure the intracellular accumulation of a substrate, which is then used to infer efflux activity. Here, we review the methods for measuring efflux and explore the most recent advances in this field, including single-cell or cell-free technologies and mass spectrometry, that are being used to provide more detailed information about efflux pump activity.
format	article
author	Jessica M. A. Blair Laura J. V. Piddock
author_facet	Jessica M. A. Blair Laura J. V. Piddock
author_sort	Jessica M. A. Blair
title	How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps
title_short	How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps
title_full	How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps
title_fullStr	How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps
title_full_unstemmed	How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps
title_sort	how to measure export via bacterial multidrug resistance efflux pumps
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/dd2a958aa61148d7ae1ba078c53bdbc7
work_keys_str_mv	AT jessicamablair howtomeasureexportviabacterialmultidrugresistanceeffluxpumps AT laurajvpiddock howtomeasureexportviabacterialmultidrugresistanceeffluxpumps
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How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps

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