Enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin

Sung-Ju Lee,1 Jong-Jin Kim,2 Kyung-Yun Kang,3 Man-Jeong Paik,1 Gwang Lee,4 Sung-Tae Yee1 1Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea; 2Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea; 3Suncheon...

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Autores principales:	Lee SJ, Kim JJ, Kang KY, Paik MJ, Lee G, Yee ST
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	antigen-delivery systems silica-coated magnetic nanoparticles ovalbumin EG-7 dendritic cell CD4+ T cell CD8+ T cell Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dd2c1590279a4f09a4327693ae81c454
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spelling	oai:doaj.org-article:dd2c1590279a4f09a4327693ae81c4542021-12-02T08:55:57ZEnhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin1178-2013https://doaj.org/article/dd2c1590279a4f09a4327693ae81c4542019-10-01T00:00:00Zhttps://www.dovepress.com/enhanced-anti-tumor-immunotherapy-by-silica-coated-magnetic-nanopartic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sung-Ju Lee,1 Jong-Jin Kim,2 Kyung-Yun Kang,3 Man-Jeong Paik,1 Gwang Lee,4 Sung-Tae Yee1 1Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea; 2Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea; 3Suncheon Research Center for Natural Medicines, Suncheon, Republic of Korea; 4Department of Physiology and Department of Biomedical Science, Ajou University School of Medicine, Suwon, Republic of KoreaCorrespondence: Sung-Tae YeeDepartment of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Republic of KoreaTel +82 61 750 3752Fax +82 61 750 3708Email sungtae@sunchon.ac.krBackground: The effective induction of an antigen-specific T cell immune response through dendritic cell activation is one of the key goals of tumor immunotherapy.Methods: In this study, efficient antigen-delivery carriers using silica-coated magnetic nanoparticles were designed and, their antigen-specific T cell immune response through dendritic cell activation investigated.Results: The results showed that the silica-coated magnetic nanoparticles with conjugated ovalbumin enhanced the production of cytokines and antigen uptake in bone marrow-derived dendritic cells. Also, this induced an antigen-specific cytotoxic T lymphocyte (CTL) immune response and activated antigen-specific Th1 cell responses, including IL-2 and IFN-γ production and proliferation. We proved that the immune-stimulatory effects of silica-coated magnetic nanoparticles with conjugated ovalbumin were efficient in inhibiting of tumor growth in EG7-OVA (mouse lymphoma-expressing ovalbumin tumor-bearing mice model).Conclusion: Therefore, the silica-coated magnetic nanoparticles with conjugated ovalbumin are expected to be useful as efficient anti-cancer immunotherapy agents.Keywords: antigen-delivery systems, silica-coated magnetic nanoparticles, ovalbumin, EG-7, dendritic cell, CD4+ T cell, CD8+ T cellLee SJKim JJKang KYPaik MJLee GYee STDove Medical Pressarticleantigen-delivery systemssilica-coated magnetic nanoparticlesovalbuminEG-7dendritic cellCD4+ T cellCD8+ T cellMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 8235-8249 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	antigen-delivery systems silica-coated magnetic nanoparticles ovalbumin EG-7 dendritic cell CD4+ T cell CD8+ T cell Medicine (General) R5-920
spellingShingle	antigen-delivery systems silica-coated magnetic nanoparticles ovalbumin EG-7 dendritic cell CD4+ T cell CD8+ T cell Medicine (General) R5-920 Lee SJ Kim JJ Kang KY Paik MJ Lee G Yee ST Enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin
description	Sung-Ju Lee,1 Jong-Jin Kim,2 Kyung-Yun Kang,3 Man-Jeong Paik,1 Gwang Lee,4 Sung-Tae Yee1 1Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea; 2Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea; 3Suncheon Research Center for Natural Medicines, Suncheon, Republic of Korea; 4Department of Physiology and Department of Biomedical Science, Ajou University School of Medicine, Suwon, Republic of KoreaCorrespondence: Sung-Tae YeeDepartment of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Republic of KoreaTel +82 61 750 3752Fax +82 61 750 3708Email sungtae@sunchon.ac.krBackground: The effective induction of an antigen-specific T cell immune response through dendritic cell activation is one of the key goals of tumor immunotherapy.Methods: In this study, efficient antigen-delivery carriers using silica-coated magnetic nanoparticles were designed and, their antigen-specific T cell immune response through dendritic cell activation investigated.Results: The results showed that the silica-coated magnetic nanoparticles with conjugated ovalbumin enhanced the production of cytokines and antigen uptake in bone marrow-derived dendritic cells. Also, this induced an antigen-specific cytotoxic T lymphocyte (CTL) immune response and activated antigen-specific Th1 cell responses, including IL-2 and IFN-γ production and proliferation. We proved that the immune-stimulatory effects of silica-coated magnetic nanoparticles with conjugated ovalbumin were efficient in inhibiting of tumor growth in EG7-OVA (mouse lymphoma-expressing ovalbumin tumor-bearing mice model).Conclusion: Therefore, the silica-coated magnetic nanoparticles with conjugated ovalbumin are expected to be useful as efficient anti-cancer immunotherapy agents.Keywords: antigen-delivery systems, silica-coated magnetic nanoparticles, ovalbumin, EG-7, dendritic cell, CD4+ T cell, CD8+ T cell
format	article
author	Lee SJ Kim JJ Kang KY Paik MJ Lee G Yee ST
author_facet	Lee SJ Kim JJ Kang KY Paik MJ Lee G Yee ST
author_sort	Lee SJ
title	Enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin
title_short	Enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin
title_full	Enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin
title_fullStr	Enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin
title_full_unstemmed	Enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin
title_sort	enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/dd2c1590279a4f09a4327693ae81c454
work_keys_str_mv	AT leesj enhancedantitumorimmunotherapybysilicacoatedmagneticnanoparticlesconjugatedwithovalbumin AT kimjj enhancedantitumorimmunotherapybysilicacoatedmagneticnanoparticlesconjugatedwithovalbumin AT kangky enhancedantitumorimmunotherapybysilicacoatedmagneticnanoparticlesconjugatedwithovalbumin AT paikmj enhancedantitumorimmunotherapybysilicacoatedmagneticnanoparticlesconjugatedwithovalbumin AT leeg enhancedantitumorimmunotherapybysilicacoatedmagneticnanoparticlesconjugatedwithovalbumin AT yeest enhancedantitumorimmunotherapybysilicacoatedmagneticnanoparticlesconjugatedwithovalbumin
_version_	1718398334784241664

Enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin

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