Fasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus

Abstract In Populus, the transcripts of fasciclin-like arabinogalactan proteins (FLAs) are accumulated in tension wood (TW) xylem, however their biological functions in TW formation are largely unknown. In this work, we demonstrated that PtFLA6, one of poplar TW-associated PtFLAs, was abundantly exp...

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Autores principales:	Haihai Wang, Yanli Jin, Cuiting Wang, Bei Li, Chunmei Jiang, Zhencang Sun, Zhiping Zhang, Fanjing Kong, Hongxia Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd332264122348a092c6cbe1870c371f
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spelling	oai:doaj.org-article:dd332264122348a092c6cbe1870c371f2021-12-02T15:06:03ZFasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus10.1038/s41598-017-06473-92045-2322https://doaj.org/article/dd332264122348a092c6cbe1870c371f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06473-9https://doaj.org/toc/2045-2322Abstract In Populus, the transcripts of fasciclin-like arabinogalactan proteins (FLAs) are accumulated in tension wood (TW) xylem, however their biological functions in TW formation are largely unknown. In this work, we demonstrated that PtFLA6, one of poplar TW-associated PtFLAs, was abundantly expressed in TW, and mainly localized in differentiating G-fibers. The bended stems of PtFLA6 antisense transgenic poplar showed decreased transcripts of PtFLAs, including PtFLA6, and reduced PtFLA6 like proteins, leading to inhibited TW differentiation and formation. We also showed that gibberellin A3 (GA3) was enriched in the xylem of TW side, accompanied with a lowered level of PtRGA1, a poplar DELLA protein. When GA3 biosynthesis was restrained in the bended poplar stems by a GA biosynthesis inhibitor (daminozide), TW formation was obviously repressed, as a result of restricted PtRGA1 degradation, and reduced PtFLA6 like proteins and PtFLA expression. Further studies indicated that PtFLAs were negatively regulated by PtRGA1. This study suggests that PtFLAs play important roles in the poplar TW formation, possibly regulated by GA signaling.Haihai WangYanli JinCuiting WangBei LiChunmei JiangZhencang SunZhiping ZhangFanjing KongHongxia ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haihai Wang Yanli Jin Cuiting Wang Bei Li Chunmei Jiang Zhencang Sun Zhiping Zhang Fanjing Kong Hongxia Zhang Fasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus
description	Abstract In Populus, the transcripts of fasciclin-like arabinogalactan proteins (FLAs) are accumulated in tension wood (TW) xylem, however their biological functions in TW formation are largely unknown. In this work, we demonstrated that PtFLA6, one of poplar TW-associated PtFLAs, was abundantly expressed in TW, and mainly localized in differentiating G-fibers. The bended stems of PtFLA6 antisense transgenic poplar showed decreased transcripts of PtFLAs, including PtFLA6, and reduced PtFLA6 like proteins, leading to inhibited TW differentiation and formation. We also showed that gibberellin A3 (GA3) was enriched in the xylem of TW side, accompanied with a lowered level of PtRGA1, a poplar DELLA protein. When GA3 biosynthesis was restrained in the bended poplar stems by a GA biosynthesis inhibitor (daminozide), TW formation was obviously repressed, as a result of restricted PtRGA1 degradation, and reduced PtFLA6 like proteins and PtFLA expression. Further studies indicated that PtFLAs were negatively regulated by PtRGA1. This study suggests that PtFLAs play important roles in the poplar TW formation, possibly regulated by GA signaling.
format	article
author	Haihai Wang Yanli Jin Cuiting Wang Bei Li Chunmei Jiang Zhencang Sun Zhiping Zhang Fanjing Kong Hongxia Zhang
author_facet	Haihai Wang Yanli Jin Cuiting Wang Bei Li Chunmei Jiang Zhencang Sun Zhiping Zhang Fanjing Kong Hongxia Zhang
author_sort	Haihai Wang
title	Fasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus
title_short	Fasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus
title_full	Fasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus
title_fullStr	Fasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus
title_full_unstemmed	Fasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus
title_sort	fasciclin-like arabinogalactan proteins, ptflas, play important roles in ga-mediated tension wood formation in populus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/dd332264122348a092c6cbe1870c371f
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Fasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus

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