P2Y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice

Abstract The role of the P2Y6 receptor in bladder function has recently attracted a great deal of attention in lower urinary tract research. We conducted this study to determine contributions of the P2Y6 receptor in lower urinary tract function of normal phenotypes by comparing P2Y6-deficient mice a...

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Autores principales:	Satoru Kira, Mitsuharu Yoshiyama, Sachiko Tsuchiya, Eiji Shigetomi, Tatsuya Miyamoto, Hiroshi Nakagomi, Keisuke Shibata, Tsutomu Mochizuki, Masayuki Takeda, Schuichi Koizumi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd34c3d825a442119d3e79bbd1e71e90
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spelling	oai:doaj.org-article:dd34c3d825a442119d3e79bbd1e71e902021-12-02T16:07:06ZP2Y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice10.1038/s41598-017-00824-22045-2322https://doaj.org/article/dd34c3d825a442119d3e79bbd1e71e902017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00824-2https://doaj.org/toc/2045-2322Abstract The role of the P2Y6 receptor in bladder function has recently attracted a great deal of attention in lower urinary tract research. We conducted this study to determine contributions of the P2Y6 receptor in lower urinary tract function of normal phenotypes by comparing P2Y6-deficient mice and wild-type mice. In in vivo experiments, P2Y6-deficient mice had more frequent micturition with smaller bladder capacity compared to wild-type mice; however, there was no difference between these groups in bladder-filling pressure/volume relationships during cystometry under decerebrate, unanaesthetized conditions. Analysis of in vivo bladder contraction revealed significant difference between the 2 groups, with P2Y6-deficient mice presenting markedly shorter bladder contraction duration but no difference in peak contraction pressure. However, analysis of in vitro experiments showed no P2Y6 involvements in contraction and relaxation of bladder muscle strips and in ATP release by mechanical stimulation of primary-cultured urothelial cells. These results suggest that the P2Y6 receptor in the central nervous system, dorsal root ganglion, or both is involved in inhibition of bladder afferent signalling or sensitivity in the pontine micturition centre and that the receptor in the detrusor may be implicated in facilitation to sustain bladder contraction force.Satoru KiraMitsuharu YoshiyamaSachiko TsuchiyaEiji ShigetomiTatsuya MiyamotoHiroshi NakagomiKeisuke ShibataTsutomu MochizukiMasayuki TakedaSchuichi KoizumiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satoru Kira Mitsuharu Yoshiyama Sachiko Tsuchiya Eiji Shigetomi Tatsuya Miyamoto Hiroshi Nakagomi Keisuke Shibata Tsutomu Mochizuki Masayuki Takeda Schuichi Koizumi P2Y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice
description	Abstract The role of the P2Y6 receptor in bladder function has recently attracted a great deal of attention in lower urinary tract research. We conducted this study to determine contributions of the P2Y6 receptor in lower urinary tract function of normal phenotypes by comparing P2Y6-deficient mice and wild-type mice. In in vivo experiments, P2Y6-deficient mice had more frequent micturition with smaller bladder capacity compared to wild-type mice; however, there was no difference between these groups in bladder-filling pressure/volume relationships during cystometry under decerebrate, unanaesthetized conditions. Analysis of in vivo bladder contraction revealed significant difference between the 2 groups, with P2Y6-deficient mice presenting markedly shorter bladder contraction duration but no difference in peak contraction pressure. However, analysis of in vitro experiments showed no P2Y6 involvements in contraction and relaxation of bladder muscle strips and in ATP release by mechanical stimulation of primary-cultured urothelial cells. These results suggest that the P2Y6 receptor in the central nervous system, dorsal root ganglion, or both is involved in inhibition of bladder afferent signalling or sensitivity in the pontine micturition centre and that the receptor in the detrusor may be implicated in facilitation to sustain bladder contraction force.
format	article
author	Satoru Kira Mitsuharu Yoshiyama Sachiko Tsuchiya Eiji Shigetomi Tatsuya Miyamoto Hiroshi Nakagomi Keisuke Shibata Tsutomu Mochizuki Masayuki Takeda Schuichi Koizumi
author_facet	Satoru Kira Mitsuharu Yoshiyama Sachiko Tsuchiya Eiji Shigetomi Tatsuya Miyamoto Hiroshi Nakagomi Keisuke Shibata Tsutomu Mochizuki Masayuki Takeda Schuichi Koizumi
author_sort	Satoru Kira
title	P2Y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice
title_short	P2Y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice
title_full	P2Y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice
title_fullStr	P2Y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice
title_full_unstemmed	P2Y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice
title_sort	p2y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/dd34c3d825a442119d3e79bbd1e71e90
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P2Y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice

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