Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats

To examine the effect of osteopontin inhibition by tranilast on liver fibrosis, four groups of rats were used throughout this study. Group I (Control group): rats received the solvent. Liver fibrosis was induced in Groups II, III, and IV by thioacetamide (TAA; 200mg/kg, ip) twice weekly for 6 weeks....

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Autores principales:	Ramadan A., Nehal A. Afifi, Nemat Z. Yassin, Rehab F. Abdel-Rahman, Sahar S. Abd El-Rahman, Hany M. Fayed
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2017
Materias:	antioxidant apoptosis liver fibrosis osteopontin Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2017.62129 https://doaj.org/article/dd3a664dfd5a44c1bdad00ec53c721de
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spelling	oai:doaj.org-article:dd3a664dfd5a44c1bdad00ec53c721de2021-12-02T13:21:34ZPotential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Ratshttps://dx.doi.org/10.21608/javs.2017.621291687-40722090-3308https://doaj.org/article/dd3a664dfd5a44c1bdad00ec53c721de2017-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_62129.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308To examine the effect of osteopontin inhibition by tranilast on liver fibrosis, four groups of rats were used throughout this study. Group I (Control group): rats received the solvent. Liver fibrosis was induced in Groups II, III, and IV by thioacetamide (TAA; 200mg/kg, ip) twice weekly for 6 weeks. Group II served as (TAA group). Groups III and IV (Treatment groups): rats were given tranilast for 6 weeks after TAA discontinuation. Liver osteopontin (OPN), transforming growth factor-β (TGF-β1), tumor necrosis factor alpha (TNF-α), alpha-smooth muscle actin (ɑ-SMA)), reduced glutathione (GSH), superoxide dismutase (SOD) and lipid peroxidation (MDA) were measured. Additionally, expression of α-smooth muscle actin (SMA) and caspase (Cas)-3 were assigned immunohistochemically. Treatment with tranilast prevented the development of hepatic fibrosis and the activation of stellate cells, and down-regulated the expression of genes for osteopontin and osteopontin-target molecules, including TGF-β and TNF-α and α-SMA.Tranilast significantly decreased MDA and increased levels of GSH, and SOD. Our findings suggest that targeting osteopontin with tranilast represents a new mode of therapy for liver fibrosis.Ramadan A.Nehal A. Afifi Nemat Z. Yassin Rehab F. Abdel-Rahman Sahar S. Abd El-RahmanHany M. Fayed Egyptian Society for Animal Managementarticleantioxidantapoptosisliver fibrosisosteopontinZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 2, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant apoptosis liver fibrosis osteopontin Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	antioxidant apoptosis liver fibrosis osteopontin Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Ramadan A. Nehal A. Afifi Nemat Z. Yassin Rehab F. Abdel-Rahman Sahar S. Abd El-Rahman Hany M. Fayed Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats
description	To examine the effect of osteopontin inhibition by tranilast on liver fibrosis, four groups of rats were used throughout this study. Group I (Control group): rats received the solvent. Liver fibrosis was induced in Groups II, III, and IV by thioacetamide (TAA; 200mg/kg, ip) twice weekly for 6 weeks. Group II served as (TAA group). Groups III and IV (Treatment groups): rats were given tranilast for 6 weeks after TAA discontinuation. Liver osteopontin (OPN), transforming growth factor-β (TGF-β1), tumor necrosis factor alpha (TNF-α), alpha-smooth muscle actin (ɑ-SMA)), reduced glutathione (GSH), superoxide dismutase (SOD) and lipid peroxidation (MDA) were measured. Additionally, expression of α-smooth muscle actin (SMA) and caspase (Cas)-3 were assigned immunohistochemically. Treatment with tranilast prevented the development of hepatic fibrosis and the activation of stellate cells, and down-regulated the expression of genes for osteopontin and osteopontin-target molecules, including TGF-β and TNF-α and α-SMA.Tranilast significantly decreased MDA and increased levels of GSH, and SOD. Our findings suggest that targeting osteopontin with tranilast represents a new mode of therapy for liver fibrosis.
format	article
author	Ramadan A. Nehal A. Afifi Nemat Z. Yassin Rehab F. Abdel-Rahman Sahar S. Abd El-Rahman Hany M. Fayed
author_facet	Ramadan A. Nehal A. Afifi Nemat Z. Yassin Rehab F. Abdel-Rahman Sahar S. Abd El-Rahman Hany M. Fayed
author_sort	Ramadan A.
title	Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats
title_short	Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats
title_full	Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats
title_fullStr	Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats
title_full_unstemmed	Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats
title_sort	potential therapeutic role of osteopontin inhibition in liver fibrosis -induced by thioacetamide in rats
publisher	Egyptian Society for Animal Management
publishDate	2017
url	https://dx.doi.org/10.21608/javs.2017.62129 https://doaj.org/article/dd3a664dfd5a44c1bdad00ec53c721de
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Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats

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