Assessing fatty acid oxidation flux in rodent cardiomyocyte models

Abstract The healthy adult heart primarily relies on fatty acid oxidation (FAO) for energy production but instantaneously adapts its substrate preference in response to physiological or pathological challenges. Accurate FAO measurements are crucial to investigate early metabolic (mal)adaptations. Wh...

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Autores principales:	M. Rech, J. J. F. P. Luiken, J. F. C. Glatz, M. van Bilsen, B. Schroen, M. Nabben
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd4037f3343f46c6953443e625a00e6c
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spelling	oai:doaj.org-article:dd4037f3343f46c6953443e625a00e6c2021-12-02T15:08:53ZAssessing fatty acid oxidation flux in rodent cardiomyocyte models10.1038/s41598-018-19478-92045-2322https://doaj.org/article/dd4037f3343f46c6953443e625a00e6c2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19478-9https://doaj.org/toc/2045-2322Abstract The healthy adult heart primarily relies on fatty acid oxidation (FAO) for energy production but instantaneously adapts its substrate preference in response to physiological or pathological challenges. Accurate FAO measurements are crucial to investigate early metabolic (mal)adaptations. While measurements in intact cardiomyocytes offer greater physiological relevance, current FAO protocols mainly employ cell-free systems and/or require expensive equipment. Here, we present an easy-to-use, inexpensive, and sensitive method to measure, compare and modulate FAO in various cardiomyocyte models. Basal FAO was 2-fold higher in fresh versus cultured adult rat cardiomyocytes (aRCM), while OXPHOS protein levels were maintained. Basal FAO was higher in cultured (3-fold) and fresh (8-fold) aRCM, versus widely used neonatal rat cardiomyocytes (nRCM) and mouse HL1 cardiomyocytes. Moreover, we utilized chemical and pharmacological treatments in order to modulate the FAO flux at different cellular signalling levels. Our data indicate that caution should be taken when studying metabolism in nRCM and HL1 cell models, as these display significantly lower FAO than aRCM. Accurate FAO measurement in cultured aRCM opens new avenues for studying the complex cardiomyocyte metabolic responses to mechanical, nutritional, pharmacological, and genetic manipulations.M. RechJ. J. F. P. LuikenJ. F. C. GlatzM. van BilsenB. SchroenM. NabbenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Rech J. J. F. P. Luiken J. F. C. Glatz M. van Bilsen B. Schroen M. Nabben Assessing fatty acid oxidation flux in rodent cardiomyocyte models
description	Abstract The healthy adult heart primarily relies on fatty acid oxidation (FAO) for energy production but instantaneously adapts its substrate preference in response to physiological or pathological challenges. Accurate FAO measurements are crucial to investigate early metabolic (mal)adaptations. While measurements in intact cardiomyocytes offer greater physiological relevance, current FAO protocols mainly employ cell-free systems and/or require expensive equipment. Here, we present an easy-to-use, inexpensive, and sensitive method to measure, compare and modulate FAO in various cardiomyocyte models. Basal FAO was 2-fold higher in fresh versus cultured adult rat cardiomyocytes (aRCM), while OXPHOS protein levels were maintained. Basal FAO was higher in cultured (3-fold) and fresh (8-fold) aRCM, versus widely used neonatal rat cardiomyocytes (nRCM) and mouse HL1 cardiomyocytes. Moreover, we utilized chemical and pharmacological treatments in order to modulate the FAO flux at different cellular signalling levels. Our data indicate that caution should be taken when studying metabolism in nRCM and HL1 cell models, as these display significantly lower FAO than aRCM. Accurate FAO measurement in cultured aRCM opens new avenues for studying the complex cardiomyocyte metabolic responses to mechanical, nutritional, pharmacological, and genetic manipulations.
format	article
author	M. Rech J. J. F. P. Luiken J. F. C. Glatz M. van Bilsen B. Schroen M. Nabben
author_facet	M. Rech J. J. F. P. Luiken J. F. C. Glatz M. van Bilsen B. Schroen M. Nabben
author_sort	M. Rech
title	Assessing fatty acid oxidation flux in rodent cardiomyocyte models
title_short	Assessing fatty acid oxidation flux in rodent cardiomyocyte models
title_full	Assessing fatty acid oxidation flux in rodent cardiomyocyte models
title_fullStr	Assessing fatty acid oxidation flux in rodent cardiomyocyte models
title_full_unstemmed	Assessing fatty acid oxidation flux in rodent cardiomyocyte models
title_sort	assessing fatty acid oxidation flux in rodent cardiomyocyte models
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/dd4037f3343f46c6953443e625a00e6c
work_keys_str_mv	AT mrech assessingfattyacidoxidationfluxinrodentcardiomyocytemodels AT jjfpluiken assessingfattyacidoxidationfluxinrodentcardiomyocytemodels AT jfcglatz assessingfattyacidoxidationfluxinrodentcardiomyocytemodels AT mvanbilsen assessingfattyacidoxidationfluxinrodentcardiomyocytemodels AT bschroen assessingfattyacidoxidationfluxinrodentcardiomyocytemodels AT mnabben assessingfattyacidoxidationfluxinrodentcardiomyocytemodels
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Assessing fatty acid oxidation flux in rodent cardiomyocyte models

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