EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS

Abstract. TRAF3IP2 gene plays an important role in the processes of inflammation and autoimmune control. TRAF3IP2 protein acts as a signal transducer from membrane receptors to the cell nucleus and is essential for the biological effects of Th17-lymphocytes. The goal of present study was to investig...

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Autores principales:	V. R. Khairutdinov, A. F. Mikhailichenko, A. A. Piskunova, N. V. Bychkova, A. V. Samtsov, E. N. Imyanitov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	psoriasis traf3ip2 gene predisposal gene expression gene polymorphism Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/dd4d014f451743a1b787323635b6582c
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spelling	oai:doaj.org-article:dd4d014f451743a1b787323635b6582c2021-11-18T08:03:42ZEXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS1563-06252313-741X10.15789/1563-0625-2011-6-597-602https://doaj.org/article/dd4d014f451743a1b787323635b6582c2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/604https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. TRAF3IP2 gene plays an important role in the processes of inflammation and autoimmune control. TRAF3IP2 protein acts as a signal transducer from membrane receptors to the cell nucleus and is essential for the biological effects of Th17-lymphocytes. The goal of present study was to investigate distribution of allelic variants of TRAF3IP2 Arg74Trp polymorphism as a risk factor in psoriasis development, and to evaluate TRAF3IP2 mRNA expression levels in psoriasis patients and healthy donors. Peripheral blood leukocytes were used as a source of DNA. Gene testing was performed for 261 patients and 482 healthy blood donors by real-time allele-specific PCR. TRAF3IP2 mRNA expression was measured in the skin biopsy samples from 36 patients with psoriasis and 14 healthy donors by means of quantitative reverse transcriptase PCR, using a paired-sample approach with SDHA reference gene. We have observed a significantly increased occurrence of rare Trp allele, with 10.5% among psoriasis patients (55/522), as compared with 7.0% (67/964) for healthy persons (OR = 1.58, 95% CI: 1.09 – 2.28, р = 0.02). A trend of higher Trp allele occurrence was revealed in patients with severe psoriasis – 21/154 (13.6%) when compared to the patients with milder course of the disease – 34/368 (9.2%) (р = 0.14). No differences were found for TRAF3IP2 mRNA expression in skin samples from psoriasis patients versus healthy persons. The association between TRAF3IP2 Trp allele and increased susceptibility to psoriasis suggests that Trp variant of this protein may display a higher enzymatic activity. Induction of inflammatory process in carriers of Trp allele would be more intensive, thus being a predisposing factor in psoriasis development. (Med. Immunol., 2011, vol. 13, N 6, pp 597-602)V. R. KhairutdinovA. F. MikhailichenkoA. A. PiskunovaN. V. BychkovaA. V. SamtsovE. N. ImyanitovSPb RAACIarticlepsoriasistraf3ip2 genepredisposalgene expressiongene polymorphismImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 13, Iss 6, Pp 597-602 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	psoriasis traf3ip2 gene predisposal gene expression gene polymorphism Immunologic diseases. Allergy RC581-607
spellingShingle	psoriasis traf3ip2 gene predisposal gene expression gene polymorphism Immunologic diseases. Allergy RC581-607 V. R. Khairutdinov A. F. Mikhailichenko A. A. Piskunova N. V. Bychkova A. V. Samtsov E. N. Imyanitov EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS
description	Abstract. TRAF3IP2 gene plays an important role in the processes of inflammation and autoimmune control. TRAF3IP2 protein acts as a signal transducer from membrane receptors to the cell nucleus and is essential for the biological effects of Th17-lymphocytes. The goal of present study was to investigate distribution of allelic variants of TRAF3IP2 Arg74Trp polymorphism as a risk factor in psoriasis development, and to evaluate TRAF3IP2 mRNA expression levels in psoriasis patients and healthy donors. Peripheral blood leukocytes were used as a source of DNA. Gene testing was performed for 261 patients and 482 healthy blood donors by real-time allele-specific PCR. TRAF3IP2 mRNA expression was measured in the skin biopsy samples from 36 patients with psoriasis and 14 healthy donors by means of quantitative reverse transcriptase PCR, using a paired-sample approach with SDHA reference gene. We have observed a significantly increased occurrence of rare Trp allele, with 10.5% among psoriasis patients (55/522), as compared with 7.0% (67/964) for healthy persons (OR = 1.58, 95% CI: 1.09 – 2.28, р = 0.02). A trend of higher Trp allele occurrence was revealed in patients with severe psoriasis – 21/154 (13.6%) when compared to the patients with milder course of the disease – 34/368 (9.2%) (р = 0.14). No differences were found for TRAF3IP2 mRNA expression in skin samples from psoriasis patients versus healthy persons. The association between TRAF3IP2 Trp allele and increased susceptibility to psoriasis suggests that Trp variant of this protein may display a higher enzymatic activity. Induction of inflammatory process in carriers of Trp allele would be more intensive, thus being a predisposing factor in psoriasis development. (Med. Immunol., 2011, vol. 13, N 6, pp 597-602)
format	article
author	V. R. Khairutdinov A. F. Mikhailichenko A. A. Piskunova N. V. Bychkova A. V. Samtsov E. N. Imyanitov
author_facet	V. R. Khairutdinov A. F. Mikhailichenko A. A. Piskunova N. V. Bychkova A. V. Samtsov E. N. Imyanitov
author_sort	V. R. Khairutdinov
title	EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS
title_short	EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS
title_full	EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS
title_fullStr	EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS
title_full_unstemmed	EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS
title_sort	expression of th17-lymphocyte adapter – traf3ip2 in the skin of psoriasis patients
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/dd4d014f451743a1b787323635b6582c
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EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS

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