Canine D163-PrP polymorphic variant does not provide complete protection against prion infection in small ruminant PrP context

Abstract E/D163 polymorphism of dog prion protein (PrP) has been recently proposed as the variant responsible for canid prion resistance. To further investigate the protective role of this variant against prion replication, the transgenic mouse model OvPrP-Tg532 expressing sheep/goat PrP carrying th...

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Autores principales:	Alba Marín-Moreno, Juan Carlos Espinosa, Patricia Aguilar-Calvo, Natalia Fernández-Borges, José Luis Pitarch, Lorenzo González, Juan María Torres
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd52988c19cf4e058e4786fdf4f8fbf6
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spelling	oai:doaj.org-article:dd52988c19cf4e058e4786fdf4f8fbf62021-12-02T16:14:08ZCanine D163-PrP polymorphic variant does not provide complete protection against prion infection in small ruminant PrP context10.1038/s41598-021-93594-x2045-2322https://doaj.org/article/dd52988c19cf4e058e4786fdf4f8fbf62021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93594-xhttps://doaj.org/toc/2045-2322Abstract E/D163 polymorphism of dog prion protein (PrP) has been recently proposed as the variant responsible for canid prion resistance. To further investigate the protective role of this variant against prion replication, the transgenic mouse model OvPrP-Tg532 expressing sheep/goat PrP carrying the substitution D162 (equivalent to D163 position of dog PrP) was generated and intracranially inoculated with a broad collection of small ruminant prion strains. OvPrP-Tg532 mice showed resistance to classical bovine spongiform encephalopathy (BSE) from sheep and some classical scrapie isolates from sheep and goat but were susceptible to ovine atypical L-BSE and numerous classical scrapie isolates. Strikingly, some of these classical scrapie isolates showed a shift in their prion strain properties. These results suggest that other PrP residues apart from E/D163 variant of dog PrP or factors distinct than PrP may participate in prion resistance of canids and that different factors may be required for D162 sheep PrP to provide effective protection to sheep against ruminant prions.Alba Marín-MorenoJuan Carlos EspinosaPatricia Aguilar-CalvoNatalia Fernández-BorgesJosé Luis PitarchLorenzo GonzálezJuan María TorresNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alba Marín-Moreno Juan Carlos Espinosa Patricia Aguilar-Calvo Natalia Fernández-Borges José Luis Pitarch Lorenzo González Juan María Torres Canine D163-PrP polymorphic variant does not provide complete protection against prion infection in small ruminant PrP context
description	Abstract E/D163 polymorphism of dog prion protein (PrP) has been recently proposed as the variant responsible for canid prion resistance. To further investigate the protective role of this variant against prion replication, the transgenic mouse model OvPrP-Tg532 expressing sheep/goat PrP carrying the substitution D162 (equivalent to D163 position of dog PrP) was generated and intracranially inoculated with a broad collection of small ruminant prion strains. OvPrP-Tg532 mice showed resistance to classical bovine spongiform encephalopathy (BSE) from sheep and some classical scrapie isolates from sheep and goat but were susceptible to ovine atypical L-BSE and numerous classical scrapie isolates. Strikingly, some of these classical scrapie isolates showed a shift in their prion strain properties. These results suggest that other PrP residues apart from E/D163 variant of dog PrP or factors distinct than PrP may participate in prion resistance of canids and that different factors may be required for D162 sheep PrP to provide effective protection to sheep against ruminant prions.
format	article
author	Alba Marín-Moreno Juan Carlos Espinosa Patricia Aguilar-Calvo Natalia Fernández-Borges José Luis Pitarch Lorenzo González Juan María Torres
author_facet	Alba Marín-Moreno Juan Carlos Espinosa Patricia Aguilar-Calvo Natalia Fernández-Borges José Luis Pitarch Lorenzo González Juan María Torres
author_sort	Alba Marín-Moreno
title	Canine D163-PrP polymorphic variant does not provide complete protection against prion infection in small ruminant PrP context
title_short	Canine D163-PrP polymorphic variant does not provide complete protection against prion infection in small ruminant PrP context
title_full	Canine D163-PrP polymorphic variant does not provide complete protection against prion infection in small ruminant PrP context
title_fullStr	Canine D163-PrP polymorphic variant does not provide complete protection against prion infection in small ruminant PrP context
title_full_unstemmed	Canine D163-PrP polymorphic variant does not provide complete protection against prion infection in small ruminant PrP context
title_sort	canine d163-prp polymorphic variant does not provide complete protection against prion infection in small ruminant prp context
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dd52988c19cf4e058e4786fdf4f8fbf6
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Canine D163-PrP polymorphic variant does not provide complete protection against prion infection in small ruminant PrP context

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