Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach

Abstract Myopia, or short-sightedness, is a highly prevalent refractive disorder in which the eye’s focal length is too short for its axial dimension in its relaxed state. High myopia is associated with increased risks of blinding ocular complications and abnormal eye shape. In addition to consisten...

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Autores principales:	Byung Soo Kang, Thomas Chuen Lam, Jimmy Ka-wai Cheung, King Kit Li, Chea-su Kee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd546f60ec30486cbb72a1b692078316
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spelling	oai:doaj.org-article:dd546f60ec30486cbb72a1b6920783162021-12-02T13:34:45ZCorneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach10.1038/s41598-021-84904-42045-2322https://doaj.org/article/dd546f60ec30486cbb72a1b6920783162021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84904-4https://doaj.org/toc/2045-2322Abstract Myopia, or short-sightedness, is a highly prevalent refractive disorder in which the eye’s focal length is too short for its axial dimension in its relaxed state. High myopia is associated with increased risks of blinding ocular complications and abnormal eye shape. In addition to consistent findings on posterior segment anomalies in high myopia (e.g., scleral remodeling), more recent biometric and biomechanical data in myopic humans and animal models also indicate anterior segment anomalies (e.g., corneal biomechanical properties). Because the cornea is the anterior-most ocular tissue, providing essential refractive power and physiological stability, it is important to understand the biochemical signaling pathway during myopia development. This study first aimed to establish the entire chicken corneal proteome. Then, using the classical form deprivation paradigm to induce high myopia in chicks, state-of-the-art bioinformatics technologies were applied to identify eight differentially expressed proteins in the highly myopic cornea. These results provide strong foundation for future corneal research, especially those using chicken as an animal model for myopia development.Byung Soo KangThomas Chuen LamJimmy Ka-wai CheungKing Kit LiChea-su KeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Byung Soo Kang Thomas Chuen Lam Jimmy Ka-wai Cheung King Kit Li Chea-su Kee Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach
description	Abstract Myopia, or short-sightedness, is a highly prevalent refractive disorder in which the eye’s focal length is too short for its axial dimension in its relaxed state. High myopia is associated with increased risks of blinding ocular complications and abnormal eye shape. In addition to consistent findings on posterior segment anomalies in high myopia (e.g., scleral remodeling), more recent biometric and biomechanical data in myopic humans and animal models also indicate anterior segment anomalies (e.g., corneal biomechanical properties). Because the cornea is the anterior-most ocular tissue, providing essential refractive power and physiological stability, it is important to understand the biochemical signaling pathway during myopia development. This study first aimed to establish the entire chicken corneal proteome. Then, using the classical form deprivation paradigm to induce high myopia in chicks, state-of-the-art bioinformatics technologies were applied to identify eight differentially expressed proteins in the highly myopic cornea. These results provide strong foundation for future corneal research, especially those using chicken as an animal model for myopia development.
format	article
author	Byung Soo Kang Thomas Chuen Lam Jimmy Ka-wai Cheung King Kit Li Chea-su Kee
author_facet	Byung Soo Kang Thomas Chuen Lam Jimmy Ka-wai Cheung King Kit Li Chea-su Kee
author_sort	Byung Soo Kang
title	Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach
title_short	Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach
title_full	Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach
title_fullStr	Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach
title_full_unstemmed	Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach
title_sort	corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free swath-ms quantification approach
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dd546f60ec30486cbb72a1b692078316
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Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach

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