Systemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model

Abstract Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage loss and reduced joint function. OA risk factors are age and obesity. Many adipokines are altered by obesity but also OA although systemic adipokine regulation in OA is not always clear. Therefore, metabolic effe...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marie-Lisa Hülser, Yubin Luo, Klaus Frommer, Rebecca Hasseli, Kernt Köhler, Magnus Diller, Lina Van Nie, Christoph Rummel, Martin Roderfeld, Elke Roeb, Georg Schett, Aline Bozec, Ulf Müller-Ladner, Elena Neumann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd5774ebdd814e90ab50873b9494e99b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dd5774ebdd814e90ab50873b9494e99b
record_format	dspace
spelling	oai:doaj.org-article:dd5774ebdd814e90ab50873b9494e99b2021-12-02T17:08:44ZSystemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model10.1038/s41598-021-96545-82045-2322https://doaj.org/article/dd5774ebdd814e90ab50873b9494e99b2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96545-8https://doaj.org/toc/2045-2322Abstract Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage loss and reduced joint function. OA risk factors are age and obesity. Many adipokines are altered by obesity but also OA although systemic adipokine regulation in OA is not always clear. Therefore, metabolic effects of diet-induced obesity on OA development as well as the influence of obesity and OA progression on systemic vs. local adipokine expression in joints were compared. C57Bl/6-mice fed with HFD (high fat diet) or normal diet prior to destabilization of the medial meniscus (DMM) were sacrificed 4/6/8 weeks after surgery. Sera were evaluated for adiponectin, leptin, visfatin, cytokines. Liver grading and staging for non-alcoholic steatohepatitis (NASH) was performed and crown-like structures (CLS) in adipose tissue measured. OA progression was scored histologically. Adipokine-expressing cells and types were evaluated by immunohistochemistry. Time-dependent changes in DMM-progression were reflected by increased systemic adiponectin levels in DMM especially combined with HFD. While HFD increased serum leptin, DMM reduced systemic leptin significantly. OA scores correlated with bodyweight, leptin and hepatic scoring. Locally, increased numbers of adiponectin- and leptin-producing fibroblasts were observed in damaged menisci but visfatin was not changed. Local adipokine expression was independent from systemic levels, suggesting different mechanisms of action.Marie-Lisa HülserYubin LuoKlaus FrommerRebecca HasseliKernt KöhlerMagnus DillerLina Van NieChristoph RummelMartin RoderfeldElke RoebGeorg SchettAline BozecUlf Müller-LadnerElena NeumannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marie-Lisa Hülser Yubin Luo Klaus Frommer Rebecca Hasseli Kernt Köhler Magnus Diller Lina Van Nie Christoph Rummel Martin Roderfeld Elke Roeb Georg Schett Aline Bozec Ulf Müller-Ladner Elena Neumann Systemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model
description	Abstract Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage loss and reduced joint function. OA risk factors are age and obesity. Many adipokines are altered by obesity but also OA although systemic adipokine regulation in OA is not always clear. Therefore, metabolic effects of diet-induced obesity on OA development as well as the influence of obesity and OA progression on systemic vs. local adipokine expression in joints were compared. C57Bl/6-mice fed with HFD (high fat diet) or normal diet prior to destabilization of the medial meniscus (DMM) were sacrificed 4/6/8 weeks after surgery. Sera were evaluated for adiponectin, leptin, visfatin, cytokines. Liver grading and staging for non-alcoholic steatohepatitis (NASH) was performed and crown-like structures (CLS) in adipose tissue measured. OA progression was scored histologically. Adipokine-expressing cells and types were evaluated by immunohistochemistry. Time-dependent changes in DMM-progression were reflected by increased systemic adiponectin levels in DMM especially combined with HFD. While HFD increased serum leptin, DMM reduced systemic leptin significantly. OA scores correlated with bodyweight, leptin and hepatic scoring. Locally, increased numbers of adiponectin- and leptin-producing fibroblasts were observed in damaged menisci but visfatin was not changed. Local adipokine expression was independent from systemic levels, suggesting different mechanisms of action.
format	article
author	Marie-Lisa Hülser Yubin Luo Klaus Frommer Rebecca Hasseli Kernt Köhler Magnus Diller Lina Van Nie Christoph Rummel Martin Roderfeld Elke Roeb Georg Schett Aline Bozec Ulf Müller-Ladner Elena Neumann
author_facet	Marie-Lisa Hülser Yubin Luo Klaus Frommer Rebecca Hasseli Kernt Köhler Magnus Diller Lina Van Nie Christoph Rummel Martin Roderfeld Elke Roeb Georg Schett Aline Bozec Ulf Müller-Ladner Elena Neumann
author_sort	Marie-Lisa Hülser
title	Systemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model
title_short	Systemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model
title_full	Systemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model
title_fullStr	Systemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model
title_full_unstemmed	Systemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model
title_sort	systemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dd5774ebdd814e90ab50873b9494e99b
work_keys_str_mv	AT marielisahulser systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT yubinluo systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT klausfrommer systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT rebeccahasseli systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT kerntkohler systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT magnusdiller systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT linavannie systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT christophrummel systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT martinroderfeld systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT elkeroeb systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT georgschett systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT alinebozec systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT ulfmullerladner systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel AT elenaneumann systemicversuslocaladipokineexpressiondiffersinacombinedobesityandosteoarthritismousemodel
_version_	1718381528068653056

Systemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model

Ejemplares similares