The MEG3 lncRNA promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for miR-21, which regulates BMPR2 levels
Preeclampsia (PE) is one of the leading causes of maternal morbidity and mortality in pregnant women. This study aimed to investigate the potential impact and regulatory mechanisms of bone morphogenetic protein receptor 2 (BMPR2) on the progression of PE. We obtained placental tissues from pregnant...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
PAGEPress Publications
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/dd5d2ec110d34e798b04a6628cb94ca1 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:dd5d2ec110d34e798b04a6628cb94ca1 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:dd5d2ec110d34e798b04a6628cb94ca12021-11-25T07:52:23ZThe MEG3 lncRNA promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for miR-21, which regulates BMPR2 levels10.4081/ejh.2021.33231121-760X2038-8306https://doaj.org/article/dd5d2ec110d34e798b04a6628cb94ca12021-11-01T00:00:00Zhttps://ejh.it/index.php/ejh/article/view/3323https://doaj.org/toc/1121-760Xhttps://doaj.org/toc/2038-8306 Preeclampsia (PE) is one of the leading causes of maternal morbidity and mortality in pregnant women. This study aimed to investigate the potential impact and regulatory mechanisms of bone morphogenetic protein receptor 2 (BMPR2) on the progression of PE. We obtained placental tissues from pregnant women with PE and normal pregnant women, and the results showed that BMPR2 was expressed at low levels in the tissue from PE women. Genetic knockdown of BMPR2 increased the proliferation and invasion of cultured trophoblast cells, whereas its overexpression reduced these characteristics. Bioinformatics analysis and luciferase reporter gene assays confirmed that BMPR2 is a direct target of miR-21. Overexpression of a miR-21 inhibitor promoted the growth and invasiveness of trophoblast cells, whereas the opposite results were observed for the miR-21 mimic. Furthermore, miR-21 was sponged by the lncRNA MEG3, and shRNA inhibition of MEG3 reduced trophoblast cell growth and invasiveness. miR-21 was upregulated in the tissues from PE women, whereas MEG3 was downregulated, and the two were negatively correlated. Collectively, this study demonstrates that the lncRNA MEG3 acts as a sponge for miR-21, which regulates BMPR2 expression and promotes trophoblast cell proliferation and invasiveness, thereby preventing the development of PE. These findings provide novel insight into a targeted therapy that could be used to treat or prevent the development of PE. Huyi LiuXiangdao Cai Jia LiuFengxiang Zhang Andong HeRuiman LiPAGEPress PublicationsarticlePreeclampsia; microRNA-21; LncRNA MEG3; Bone morphogenetic protein receptor 2; trophoblast cellBiology (General)QH301-705.5ENEuropean Journal of Histochemistry , Vol 65, Iss 4 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Preeclampsia; microRNA-21; LncRNA MEG3; Bone morphogenetic protein receptor 2; trophoblast cell Biology (General) QH301-705.5 |
| spellingShingle |
Preeclampsia; microRNA-21; LncRNA MEG3; Bone morphogenetic protein receptor 2; trophoblast cell Biology (General) QH301-705.5 Huyi Liu Xiangdao Cai Jia Liu Fengxiang Zhang Andong He Ruiman Li The MEG3 lncRNA promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for miR-21, which regulates BMPR2 levels |
| description |
Preeclampsia (PE) is one of the leading causes of maternal morbidity and mortality in pregnant women. This study aimed to investigate the potential impact and regulatory mechanisms of bone morphogenetic protein receptor 2 (BMPR2) on the progression of PE. We obtained placental tissues from pregnant women with PE and normal pregnant women, and the results showed that BMPR2 was expressed at low levels in the tissue from PE women. Genetic knockdown of BMPR2 increased the proliferation and invasion of cultured trophoblast cells, whereas its overexpression reduced these characteristics. Bioinformatics analysis and luciferase reporter gene assays confirmed that BMPR2 is a direct target of miR-21. Overexpression of a miR-21 inhibitor promoted the growth and invasiveness of trophoblast cells, whereas the opposite results were observed for the miR-21 mimic. Furthermore, miR-21 was sponged by the lncRNA MEG3, and shRNA inhibition of MEG3 reduced trophoblast cell growth and invasiveness. miR-21 was upregulated in the tissues from PE women, whereas MEG3 was downregulated, and the two were negatively correlated. Collectively, this study demonstrates that the lncRNA MEG3 acts as a sponge for miR-21, which regulates BMPR2 expression and promotes trophoblast cell proliferation and invasiveness, thereby preventing the development of PE. These findings provide novel insight into a targeted therapy that could be used to treat or prevent the development of PE.
|
| format |
article |
| author |
Huyi Liu Xiangdao Cai Jia Liu Fengxiang Zhang Andong He Ruiman Li |
| author_facet |
Huyi Liu Xiangdao Cai Jia Liu Fengxiang Zhang Andong He Ruiman Li |
| author_sort |
Huyi Liu |
| title |
The MEG3 lncRNA promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for miR-21, which regulates BMPR2 levels |
| title_short |
The MEG3 lncRNA promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for miR-21, which regulates BMPR2 levels |
| title_full |
The MEG3 lncRNA promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for miR-21, which regulates BMPR2 levels |
| title_fullStr |
The MEG3 lncRNA promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for miR-21, which regulates BMPR2 levels |
| title_full_unstemmed |
The MEG3 lncRNA promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for miR-21, which regulates BMPR2 levels |
| title_sort |
meg3 lncrna promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for mir-21, which regulates bmpr2 levels |
| publisher |
PAGEPress Publications |
| publishDate |
2021 |
| url |
https://doaj.org/article/dd5d2ec110d34e798b04a6628cb94ca1 |
| work_keys_str_mv |
AT huyiliu themeg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT xiangdaocai themeg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT jialiu themeg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT fengxiangzhang themeg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT andonghe themeg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT ruimanli themeg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT huyiliu meg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT xiangdaocai meg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT jialiu meg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT fengxiangzhang meg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT andonghe meg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels AT ruimanli meg3lncrnapromotestrophoblasticcellgrowthandinvasivenessinpreeclampsiabyactingasaspongeformir21whichregulatesbmpr2levels |
| _version_ |
1718413651794198528 |