Functional human genes typically exhibit epigenetic conservation.

Recent DepMap CRISPR-Cas9 single gene disruptions have identified genes more essential to proliferation in tissue culture. It would be valuable to translate these finding with measurements more practical for human tissues. Here we show that DepMap essential genes and other literature curated functio...

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Autores principales:	Daniel Rud, Paul Marjoram, Kimberly Siegmund, Darryl Shibata
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd5e8a0b733444d29d5c1894c5fc6331
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spelling	oai:doaj.org-article:dd5e8a0b733444d29d5c1894c5fc63312021-12-02T20:08:17ZFunctional human genes typically exhibit epigenetic conservation.1932-620310.1371/journal.pone.0253250https://doaj.org/article/dd5e8a0b733444d29d5c1894c5fc63312021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253250https://doaj.org/toc/1932-6203Recent DepMap CRISPR-Cas9 single gene disruptions have identified genes more essential to proliferation in tissue culture. It would be valuable to translate these finding with measurements more practical for human tissues. Here we show that DepMap essential genes and other literature curated functional genes exhibit cell-specific preferential epigenetic conservation when DNA methylation measurements are compared between replicate cell lines and between intestinal crypts from the same individual. Culture experiments indicate that epigenetic drift accumulates through time with smaller differences in more functional genes. In NCI-60 cell lines, greater targeted gene conservation correlated with greater drug sensitivity. These studies indicate that two measurements separated in time allow normal or neoplastic cells to signal through conservation which human genes are more essential to their survival in vitro or in vivo.Daniel RudPaul MarjoramKimberly SiegmundDarryl ShibataPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0253250 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Rud Paul Marjoram Kimberly Siegmund Darryl Shibata Functional human genes typically exhibit epigenetic conservation.
description	Recent DepMap CRISPR-Cas9 single gene disruptions have identified genes more essential to proliferation in tissue culture. It would be valuable to translate these finding with measurements more practical for human tissues. Here we show that DepMap essential genes and other literature curated functional genes exhibit cell-specific preferential epigenetic conservation when DNA methylation measurements are compared between replicate cell lines and between intestinal crypts from the same individual. Culture experiments indicate that epigenetic drift accumulates through time with smaller differences in more functional genes. In NCI-60 cell lines, greater targeted gene conservation correlated with greater drug sensitivity. These studies indicate that two measurements separated in time allow normal or neoplastic cells to signal through conservation which human genes are more essential to their survival in vitro or in vivo.
format	article
author	Daniel Rud Paul Marjoram Kimberly Siegmund Darryl Shibata
author_facet	Daniel Rud Paul Marjoram Kimberly Siegmund Darryl Shibata
author_sort	Daniel Rud
title	Functional human genes typically exhibit epigenetic conservation.
title_short	Functional human genes typically exhibit epigenetic conservation.
title_full	Functional human genes typically exhibit epigenetic conservation.
title_fullStr	Functional human genes typically exhibit epigenetic conservation.
title_full_unstemmed	Functional human genes typically exhibit epigenetic conservation.
title_sort	functional human genes typically exhibit epigenetic conservation.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/dd5e8a0b733444d29d5c1894c5fc6331
work_keys_str_mv	AT danielrud functionalhumangenestypicallyexhibitepigeneticconservation AT paulmarjoram functionalhumangenestypicallyexhibitepigeneticconservation AT kimberlysiegmund functionalhumangenestypicallyexhibitepigeneticconservation AT darrylshibata functionalhumangenestypicallyexhibitepigeneticconservation
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Functional human genes typically exhibit epigenetic conservation.

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