The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts

Bozena Novotna,1 Vit Herynek,2 Pavel Rossner Jr,1 Karolina Turnovcova,3 Pavla Jendelova3 1Department of Genetic Toxicology and Nanotoxicology, Institute of Experimental Medicine AS CR, v.v.i., 2Magnetic Resonance Unit, Radiodiagnostic and Interventional Radiology Department, Institute for Clinical...

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Autores principales:	Novotna B, Herynek V, Rossner P Jr, Turnovcova K, Jendelova P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	nanoparticles MRI comet assay genotoxicity lipid peroxidation protein oxidative damage cell transplantation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dd61504380aa479c96665887b42c5527
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spelling	oai:doaj.org-article:dd61504380aa479c96665887b42c55272021-12-02T02:10:25ZThe effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts1178-2013https://doaj.org/article/dd61504380aa479c96665887b42c55272017-06-01T00:00:00Zhttps://www.dovepress.com/the-effects-of-grafted-mesenchymal-stem-cells-labeled-with-iron-oxide--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Bozena Novotna,1 Vit Herynek,2 Pavel Rossner Jr,1 Karolina Turnovcova,3 Pavla Jendelova3 1Department of Genetic Toxicology and Nanotoxicology, Institute of Experimental Medicine AS CR, v.v.i., 2Magnetic Resonance Unit, Radiodiagnostic and Interventional Radiology Department, Institute for Clinical and Experimental Medicine, 3Department of Tissue Culture and Stem Cells, Institute of Experimental Medicine AS CR, v.v.i., Prague, Czech Republic Introduction: Rat mesenchymal stem cells (rMSCs) labeled with 1) poly-l-lysine-coated superparamagnetic iron oxide nanoparticles or 2) silica-coated cobalt-zinc-iron nanoparticles were implanted into the left brain hemisphere of rats, to assess their effects on the levels of oxidative damage to biological macromolecules in brain tissue.Methods: Controls were implanted with unlabeled rMSCs. Animals were sacrificed 24 hours or 4 weeks after the treatment, and the implantation site along with the surrounding tissue was isolated from the brain. At the same intervals, parallel groups of animals were scanned in vivo by magnetic resonance imaging (MRI). The comet assay with enzymes of excision DNA repair (endonuclease III and formamidopyrimidine-DNA glycosylase) was used to analyze breaks and oxidative damage to DNA in the brain tissue. Oxidative damage to proteins and lipids was determined by measuring the levels of carbonyl groups and 15-F2t-isoprostane (enzyme-linked immunosorbent assay). MRI displayed implants of labeled cells as extensive hypointense areas in the brain tissue. In histological sections, the expression of glial fibrillary acidic protein and CD68 was analyzed to detect astrogliosis and inflammatory response.Results: Both contrast labels caused a similar response in the T2-weighted magnetic resonance (MR) image and the signal was clearly visible within 4 weeks after implantation of rMSCs. No increase of oxidative damage to DNA, lipids, or proteins over the control values was detected in any sample of brain tissue from the treated animals. Also, immunohistochemistry did not indicate any serious tissue impairment around the graft.Conclusion: Both tested types of nanoparticles appear to be prospective and safe labels for tracking the transplanted cells by MR. Keywords: MRI, comet assay, genotoxicity, lipid peroxidation, protein oxidative damage, cell transplantation Novotna BHerynek VRossner P JrTurnovcova KJendelova PDove Medical PressarticlenanoparticlesMRIcomet assaygenotoxicitylipid peroxidationprotein oxidative damagecell transplantationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4519-4526 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoparticles MRI comet assay genotoxicity lipid peroxidation protein oxidative damage cell transplantation Medicine (General) R5-920
spellingShingle	nanoparticles MRI comet assay genotoxicity lipid peroxidation protein oxidative damage cell transplantation Medicine (General) R5-920 Novotna B Herynek V Rossner P Jr Turnovcova K Jendelova P The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts
description	Bozena Novotna,1 Vit Herynek,2 Pavel Rossner Jr,1 Karolina Turnovcova,3 Pavla Jendelova3 1Department of Genetic Toxicology and Nanotoxicology, Institute of Experimental Medicine AS CR, v.v.i., 2Magnetic Resonance Unit, Radiodiagnostic and Interventional Radiology Department, Institute for Clinical and Experimental Medicine, 3Department of Tissue Culture and Stem Cells, Institute of Experimental Medicine AS CR, v.v.i., Prague, Czech Republic Introduction: Rat mesenchymal stem cells (rMSCs) labeled with 1) poly-l-lysine-coated superparamagnetic iron oxide nanoparticles or 2) silica-coated cobalt-zinc-iron nanoparticles were implanted into the left brain hemisphere of rats, to assess their effects on the levels of oxidative damage to biological macromolecules in brain tissue.Methods: Controls were implanted with unlabeled rMSCs. Animals were sacrificed 24 hours or 4 weeks after the treatment, and the implantation site along with the surrounding tissue was isolated from the brain. At the same intervals, parallel groups of animals were scanned in vivo by magnetic resonance imaging (MRI). The comet assay with enzymes of excision DNA repair (endonuclease III and formamidopyrimidine-DNA glycosylase) was used to analyze breaks and oxidative damage to DNA in the brain tissue. Oxidative damage to proteins and lipids was determined by measuring the levels of carbonyl groups and 15-F2t-isoprostane (enzyme-linked immunosorbent assay). MRI displayed implants of labeled cells as extensive hypointense areas in the brain tissue. In histological sections, the expression of glial fibrillary acidic protein and CD68 was analyzed to detect astrogliosis and inflammatory response.Results: Both contrast labels caused a similar response in the T2-weighted magnetic resonance (MR) image and the signal was clearly visible within 4 weeks after implantation of rMSCs. No increase of oxidative damage to DNA, lipids, or proteins over the control values was detected in any sample of brain tissue from the treated animals. Also, immunohistochemistry did not indicate any serious tissue impairment around the graft.Conclusion: Both tested types of nanoparticles appear to be prospective and safe labels for tracking the transplanted cells by MR. Keywords: MRI, comet assay, genotoxicity, lipid peroxidation, protein oxidative damage, cell transplantation
format	article
author	Novotna B Herynek V Rossner P Jr Turnovcova K Jendelova P
author_facet	Novotna B Herynek V Rossner P Jr Turnovcova K Jendelova P
author_sort	Novotna B
title	The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts
title_short	The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts
title_full	The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts
title_fullStr	The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts
title_full_unstemmed	The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts
title_sort	effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/dd61504380aa479c96665887b42c5527
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The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts

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