A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803

Abstract Phosphoketolase (PKET) pathway is predominant in cyanobacteria (around 98%) but current opinion is that it is virtually inactive under autotrophic ambient CO2 condition (AC-auto). This creates an evolutionary paradox due to the existence of PKET pathway in obligatory photoautotrophs. We aim...

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Autores principales:	Anushree Bachhar, Jiri Jablonsky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd6687e5bdcf48bea5192d6a4130df1d
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spelling	oai:doaj.org-article:dd6687e5bdcf48bea5192d6a4130df1d2021-12-02T13:34:09ZA new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 680310.1038/s41598-020-78475-z2045-2322https://doaj.org/article/dd6687e5bdcf48bea5192d6a4130df1d2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78475-zhttps://doaj.org/toc/2045-2322Abstract Phosphoketolase (PKET) pathway is predominant in cyanobacteria (around 98%) but current opinion is that it is virtually inactive under autotrophic ambient CO2 condition (AC-auto). This creates an evolutionary paradox due to the existence of PKET pathway in obligatory photoautotrophs. We aim to answer the paradox with the aid of bioinformatic analysis along with metabolic, transcriptomic, fluxomic and mutant data integrated into a multi-level kinetic model. We discussed the problems linked to neglected isozyme, pket2 (sll0529) and inconsistencies towards the explanation of residual flux via PKET pathway in the case of silenced pket1 (slr0453) in Synechocystis sp. PCC 6803. Our in silico analysis showed: (1) 17% flux reduction via RuBisCO for Δpket1 under AC-auto, (2) 11.2–14.3% growth decrease for Δpket2 in turbulent AC-auto, and (3) flux via PKET pathway reaching up to 252% of the flux via phosphoglycerate mutase under AC-auto. All results imply that PKET pathway plays a crucial role under AC-auto by mitigating the decarboxylation occurring in OPP pathway and conversion of pyruvate to acetyl CoA linked to EMP glycolysis under the carbon scarce environment. Finally, our model predicted that PKETs have low affinity to S7P as a substrate.Anushree BachharJiri JablonskyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anushree Bachhar Jiri Jablonsky A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803
description	Abstract Phosphoketolase (PKET) pathway is predominant in cyanobacteria (around 98%) but current opinion is that it is virtually inactive under autotrophic ambient CO2 condition (AC-auto). This creates an evolutionary paradox due to the existence of PKET pathway in obligatory photoautotrophs. We aim to answer the paradox with the aid of bioinformatic analysis along with metabolic, transcriptomic, fluxomic and mutant data integrated into a multi-level kinetic model. We discussed the problems linked to neglected isozyme, pket2 (sll0529) and inconsistencies towards the explanation of residual flux via PKET pathway in the case of silenced pket1 (slr0453) in Synechocystis sp. PCC 6803. Our in silico analysis showed: (1) 17% flux reduction via RuBisCO for Δpket1 under AC-auto, (2) 11.2–14.3% growth decrease for Δpket2 in turbulent AC-auto, and (3) flux via PKET pathway reaching up to 252% of the flux via phosphoglycerate mutase under AC-auto. All results imply that PKET pathway plays a crucial role under AC-auto by mitigating the decarboxylation occurring in OPP pathway and conversion of pyruvate to acetyl CoA linked to EMP glycolysis under the carbon scarce environment. Finally, our model predicted that PKETs have low affinity to S7P as a substrate.
format	article
author	Anushree Bachhar Jiri Jablonsky
author_facet	Anushree Bachhar Jiri Jablonsky
author_sort	Anushree Bachhar
title	A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803
title_short	A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803
title_full	A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803
title_fullStr	A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803
title_full_unstemmed	A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803
title_sort	new insight into role of phosphoketolase pathway in synechocystis sp. pcc 6803
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/dd6687e5bdcf48bea5192d6a4130df1d
work_keys_str_mv	AT anushreebachhar anewinsightintoroleofphosphoketolasepathwayinsynechocystissppcc6803 AT jirijablonsky anewinsightintoroleofphosphoketolasepathwayinsynechocystissppcc6803 AT anushreebachhar newinsightintoroleofphosphoketolasepathwayinsynechocystissppcc6803 AT jirijablonsky newinsightintoroleofphosphoketolasepathwayinsynechocystissppcc6803
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A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803

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