Visualization of elastin using cardiac magnetic resonance imaging after myocardial infarction as inflammatory response

Abstract The aim of this study was to investigate the merits of magnetic resonance imaging (MRI) using an elastin-binding contrast agent after myocardial infarction in mouse models with deletions of monocyte populations. Permanent ligation of the left anterior descending (LAD) artery was conducted i...

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Autores principales:	Britta Elkenhans, Andrea Protti, Ajay Shah, David Onthank, René Botnar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd67326f0d7e4535a0fa1e0b8d3cdf11
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spelling	oai:doaj.org-article:dd67326f0d7e4535a0fa1e0b8d3cdf112021-12-02T15:49:43ZVisualization of elastin using cardiac magnetic resonance imaging after myocardial infarction as inflammatory response10.1038/s41598-021-90092-y2045-2322https://doaj.org/article/dd67326f0d7e4535a0fa1e0b8d3cdf112021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90092-yhttps://doaj.org/toc/2045-2322Abstract The aim of this study was to investigate the merits of magnetic resonance imaging (MRI) using an elastin-binding contrast agent after myocardial infarction in mouse models with deletions of monocyte populations. Permanent ligation of the left anterior descending (LAD) artery was conducted in 10 wild-type mice and 10 each of three knockout models: CX3CR−/−, CCR2−/−, and MCP-1−/−. At 7 days and 30 days after permanent ligation, cardiac MRI was performed with a 7 T-Bruker horizontal scanner for in vivo detection of elastin with an elastin/tropoelastin-specific contrast agent (ESMA). Histology was performed with staining for elastin, collagen I and III, and F4/80. Real-time PCR was conducted to quantify the expression of genes for collagen I and III, F4/80, and tumor necrosis factor alpha (TNFα). Histological and ESMA-indicated elastin areas were strongly correlated (r = 0.8). 30 days after permanent ligation, CCR2-deficient mice demonstrated higher elastin levels in the scar relative to MCP-1−/− (p < 0.04) and wild-type mice (p < 0.02). The ejection fraction was lower in CCR2-deficient mice. In vivo MRI in mouse models of MI can detect elastin deposition after myocardial infarction, highlighting the pivotal role of elastin in myocardial remodeling in mouse models with deletions of monocyte populations.Britta ElkenhansAndrea ProttiAjay ShahDavid OnthankRené BotnarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Britta Elkenhans Andrea Protti Ajay Shah David Onthank René Botnar Visualization of elastin using cardiac magnetic resonance imaging after myocardial infarction as inflammatory response
description	Abstract The aim of this study was to investigate the merits of magnetic resonance imaging (MRI) using an elastin-binding contrast agent after myocardial infarction in mouse models with deletions of monocyte populations. Permanent ligation of the left anterior descending (LAD) artery was conducted in 10 wild-type mice and 10 each of three knockout models: CX3CR−/−, CCR2−/−, and MCP-1−/−. At 7 days and 30 days after permanent ligation, cardiac MRI was performed with a 7 T-Bruker horizontal scanner for in vivo detection of elastin with an elastin/tropoelastin-specific contrast agent (ESMA). Histology was performed with staining for elastin, collagen I and III, and F4/80. Real-time PCR was conducted to quantify the expression of genes for collagen I and III, F4/80, and tumor necrosis factor alpha (TNFα). Histological and ESMA-indicated elastin areas were strongly correlated (r = 0.8). 30 days after permanent ligation, CCR2-deficient mice demonstrated higher elastin levels in the scar relative to MCP-1−/− (p < 0.04) and wild-type mice (p < 0.02). The ejection fraction was lower in CCR2-deficient mice. In vivo MRI in mouse models of MI can detect elastin deposition after myocardial infarction, highlighting the pivotal role of elastin in myocardial remodeling in mouse models with deletions of monocyte populations.
format	article
author	Britta Elkenhans Andrea Protti Ajay Shah David Onthank René Botnar
author_facet	Britta Elkenhans Andrea Protti Ajay Shah David Onthank René Botnar
author_sort	Britta Elkenhans
title	Visualization of elastin using cardiac magnetic resonance imaging after myocardial infarction as inflammatory response
title_short	Visualization of elastin using cardiac magnetic resonance imaging after myocardial infarction as inflammatory response
title_full	Visualization of elastin using cardiac magnetic resonance imaging after myocardial infarction as inflammatory response
title_fullStr	Visualization of elastin using cardiac magnetic resonance imaging after myocardial infarction as inflammatory response
title_full_unstemmed	Visualization of elastin using cardiac magnetic resonance imaging after myocardial infarction as inflammatory response
title_sort	visualization of elastin using cardiac magnetic resonance imaging after myocardial infarction as inflammatory response
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dd67326f0d7e4535a0fa1e0b8d3cdf11
work_keys_str_mv	AT brittaelkenhans visualizationofelastinusingcardiacmagneticresonanceimagingaftermyocardialinfarctionasinflammatoryresponse AT andreaprotti visualizationofelastinusingcardiacmagneticresonanceimagingaftermyocardialinfarctionasinflammatoryresponse AT ajayshah visualizationofelastinusingcardiacmagneticresonanceimagingaftermyocardialinfarctionasinflammatoryresponse AT davidonthank visualizationofelastinusingcardiacmagneticresonanceimagingaftermyocardialinfarctionasinflammatoryresponse AT renebotnar visualizationofelastinusingcardiacmagneticresonanceimagingaftermyocardialinfarctionasinflammatoryresponse
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Visualization of elastin using cardiac magnetic resonance imaging after myocardial infarction as inflammatory response

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