cAMP Receptor Protein Controls <named-content content-type="genus-species">Vibrio cholerae</named-content> Gene Expression in Response to Host Colonization

ABSTRACT The bacterium Vibrio cholerae is native to aquatic environments and can switch lifestyles to cause disease in humans. Lifestyle switching requires modulation of genetic systems for quorum sensing, intestinal colonization, and toxin production. Much of this regulation occurs at the level of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jainaba Manneh-Roussel, James R. J. Haycocks, Andrés Magán, Nicolas Perez-Soto, Kerstin Voelz, Andrew Camilli, Anne-Marie Krachler, David C. Grainger
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Vibrio biochemistry gene regulation genome analysis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dd6c3f43ddf0440d9d29e97d2d7e8a07
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dd6c3f43ddf0440d9d29e97d2d7e8a07
record_format	dspace
spelling	oai:doaj.org-article:dd6c3f43ddf0440d9d29e97d2d7e8a072021-11-15T16:00:14ZcAMP Receptor Protein Controls <named-content content-type="genus-species">Vibrio cholerae</named-content> Gene Expression in Response to Host Colonization10.1128/mBio.00966-182150-7511https://doaj.org/article/dd6c3f43ddf0440d9d29e97d2d7e8a072018-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00966-18https://doaj.org/toc/2150-7511ABSTRACT The bacterium Vibrio cholerae is native to aquatic environments and can switch lifestyles to cause disease in humans. Lifestyle switching requires modulation of genetic systems for quorum sensing, intestinal colonization, and toxin production. Much of this regulation occurs at the level of gene expression and is controlled by transcription factors. In this work, we have mapped the binding of cAMP receptor protein (CRP) and RNA polymerase across the V. cholerae genome. We show that CRP is an integral component of the regulatory network that controls lifestyle switching. Focusing on a locus necessary for toxin transport, we demonstrate CRP-dependent regulation of gene expression in response to host colonization. Examination of further CRP-targeted genes reveals that this behavior is commonplace. Hence, CRP is a key regulator of many V. cholerae genes in response to lifestyle changes. IMPORTANCE Cholera is an infectious disease that is caused by the bacterium Vibrio cholerae. Best known for causing disease in humans, the bacterium is most commonly found in aquatic ecosystems. Hence, humans acquire cholera following ingestion of food or water contaminated with V. cholerae. Transition between an aquatic environment and a human host triggers a lifestyle switch that involves reprogramming of V. cholerae gene expression patterns. This process is controlled by a network of transcription factors. In this paper, we show that the cAMP receptor protein (CRP) is a key regulator of V. cholerae gene expression in response to lifestyle changes.Jainaba Manneh-RousselJames R. J. HaycocksAndrés MagánNicolas Perez-SotoKerstin VoelzAndrew CamilliAnne-Marie KrachlerDavid C. GraingerAmerican Society for MicrobiologyarticleVibriobiochemistrygene regulationgenome analysisMicrobiologyQR1-502ENmBio, Vol 9, Iss 4 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Vibrio biochemistry gene regulation genome analysis Microbiology QR1-502
spellingShingle	Vibrio biochemistry gene regulation genome analysis Microbiology QR1-502 Jainaba Manneh-Roussel James R. J. Haycocks Andrés Magán Nicolas Perez-Soto Kerstin Voelz Andrew Camilli Anne-Marie Krachler David C. Grainger cAMP Receptor Protein Controls <named-content content-type="genus-species">Vibrio cholerae</named-content> Gene Expression in Response to Host Colonization
description	ABSTRACT The bacterium Vibrio cholerae is native to aquatic environments and can switch lifestyles to cause disease in humans. Lifestyle switching requires modulation of genetic systems for quorum sensing, intestinal colonization, and toxin production. Much of this regulation occurs at the level of gene expression and is controlled by transcription factors. In this work, we have mapped the binding of cAMP receptor protein (CRP) and RNA polymerase across the V. cholerae genome. We show that CRP is an integral component of the regulatory network that controls lifestyle switching. Focusing on a locus necessary for toxin transport, we demonstrate CRP-dependent regulation of gene expression in response to host colonization. Examination of further CRP-targeted genes reveals that this behavior is commonplace. Hence, CRP is a key regulator of many V. cholerae genes in response to lifestyle changes. IMPORTANCE Cholera is an infectious disease that is caused by the bacterium Vibrio cholerae. Best known for causing disease in humans, the bacterium is most commonly found in aquatic ecosystems. Hence, humans acquire cholera following ingestion of food or water contaminated with V. cholerae. Transition between an aquatic environment and a human host triggers a lifestyle switch that involves reprogramming of V. cholerae gene expression patterns. This process is controlled by a network of transcription factors. In this paper, we show that the cAMP receptor protein (CRP) is a key regulator of V. cholerae gene expression in response to lifestyle changes.
format	article
author	Jainaba Manneh-Roussel James R. J. Haycocks Andrés Magán Nicolas Perez-Soto Kerstin Voelz Andrew Camilli Anne-Marie Krachler David C. Grainger
author_facet	Jainaba Manneh-Roussel James R. J. Haycocks Andrés Magán Nicolas Perez-Soto Kerstin Voelz Andrew Camilli Anne-Marie Krachler David C. Grainger
author_sort	Jainaba Manneh-Roussel
title	cAMP Receptor Protein Controls <named-content content-type="genus-species">Vibrio cholerae</named-content> Gene Expression in Response to Host Colonization
title_short	cAMP Receptor Protein Controls <named-content content-type="genus-species">Vibrio cholerae</named-content> Gene Expression in Response to Host Colonization
title_full	cAMP Receptor Protein Controls <named-content content-type="genus-species">Vibrio cholerae</named-content> Gene Expression in Response to Host Colonization
title_fullStr	cAMP Receptor Protein Controls <named-content content-type="genus-species">Vibrio cholerae</named-content> Gene Expression in Response to Host Colonization
title_full_unstemmed	cAMP Receptor Protein Controls <named-content content-type="genus-species">Vibrio cholerae</named-content> Gene Expression in Response to Host Colonization
title_sort	camp receptor protein controls <named-content content-type="genus-species">vibrio cholerae</named-content> gene expression in response to host colonization
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/dd6c3f43ddf0440d9d29e97d2d7e8a07
work_keys_str_mv	AT jainabamannehroussel campreceptorproteincontrolsnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentgeneexpressioninresponsetohostcolonization AT jamesrjhaycocks campreceptorproteincontrolsnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentgeneexpressioninresponsetohostcolonization AT andresmagan campreceptorproteincontrolsnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentgeneexpressioninresponsetohostcolonization AT nicolasperezsoto campreceptorproteincontrolsnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentgeneexpressioninresponsetohostcolonization AT kerstinvoelz campreceptorproteincontrolsnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentgeneexpressioninresponsetohostcolonization AT andrewcamilli campreceptorproteincontrolsnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentgeneexpressioninresponsetohostcolonization AT annemariekrachler campreceptorproteincontrolsnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentgeneexpressioninresponsetohostcolonization AT davidcgrainger campreceptorproteincontrolsnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentgeneexpressioninresponsetohostcolonization
_version_	1718427000227495936

cAMP Receptor Protein Controls <named-content content-type="genus-species">Vibrio cholerae</named-content> Gene Expression in Response to Host Colonization

Ejemplares similares