Development of a generic β-lactamase screening system for improved signal peptides for periplasmic targeting of recombinant proteins in Escherichia coli

Abstract Targeting of recombinant proteins to the Escherichia coli periplasm is a desirable industrial processing tool to allow formation of disulphide bonds, aid folding and simplify recovery. Proteins are targeted across the inner membrane to the periplasm by an N-terminal signal peptide. The sequ...

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Autores principales: Tania Selas Castiñeiras, Steven G. Williams, Antony Hitchcock, Jeffrey A. Cole, Daniel C. Smith, Tim W. Overton
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/dd6e1f24aab44fdcaedaf5ac20461022
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