Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model

The use of vaginal surgical mesh to treat pelvic organ prolapse (POP) has been associated with high rates of mesh-related complications. In the present study, we prepared new kinds of meshes based on bacterial cellulose (BC) and collagen-coated BC (BCCOL) using a laser cutting method and perforation...

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Autores principales:	Chen Lai, Shu-Jiang Zhang, Xuan-Chen Chen, Li-Yuan Sheng, Tian-Wei Qi, Le-Ping Yan
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Pelvic organ prolapse Cellulose-based vaginal mesh In vivo evaluation Tissue integration Inflammatory reaction Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dd70ef67c1164f659848ef8af380dc66
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spelling	oai:doaj.org-article:dd70ef67c1164f659848ef8af380dc662021-12-02T05:03:34ZDevelopment of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model2590-006410.1016/j.mtbio.2021.100172https://doaj.org/article/dd70ef67c1164f659848ef8af380dc662021-09-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590006421000806https://doaj.org/toc/2590-0064The use of vaginal surgical mesh to treat pelvic organ prolapse (POP) has been associated with high rates of mesh-related complications. In the present study, we prepared new kinds of meshes based on bacterial cellulose (BC) and collagen-coated BC (BCCOL) using a laser cutting method and perforation technique. The mechanical properties of pre-implanted BC meshes, including breaking strength, suture strength and rigidity, were equal to or exceeded those of available clinically used polypropylene meshes. An in vitro cellular assay revealed that BCCOL meshes exhibited enhanced biocompatibility by increasing collagen secretion and cell adhesion. Both BC and BCCOL meshes only caused weak inflammation and were surrounded by newly formed connective tissue composed of type I collagen after implantation in a rabbit subcutaneous model for one week, demonstrating that the novel mesh is fully biocompatible and can integrate into surrounding tissues. Furthermore, a long-term (ninety days) ewe vaginal implantation model was used to evaluate foreign body reactions and suitability of BC and BCCOL meshes as vaginal meshes. The results showed that the tissue surrounding the BC meshes returned to its original physiology as muscle tissue, indicating the excellent integration of BC meshes into the surrounding tissues without triggering severe local inflammatory response post-implantation. The collagen coating appeared to induce a chronic inflammatory response due to glutaraldehyde remnants. The present exploratory research demonstrated that the developed BC mesh might be a suitable candidate for treating POP.Chen LaiShu-Jiang ZhangXuan-Chen ChenLi-Yuan ShengTian-Wei QiLe-Ping YanElsevierarticlePelvic organ prolapseCellulose-based vaginal meshIn vivo evaluationTissue integrationInflammatory reactionMedicine (General)R5-920Biology (General)QH301-705.5ENMaterials Today Bio, Vol 12, Iss , Pp 100172- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pelvic organ prolapse Cellulose-based vaginal mesh In vivo evaluation Tissue integration Inflammatory reaction Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	Pelvic organ prolapse Cellulose-based vaginal mesh In vivo evaluation Tissue integration Inflammatory reaction Medicine (General) R5-920 Biology (General) QH301-705.5 Chen Lai Shu-Jiang Zhang Xuan-Chen Chen Li-Yuan Sheng Tian-Wei Qi Le-Ping Yan Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
description	The use of vaginal surgical mesh to treat pelvic organ prolapse (POP) has been associated with high rates of mesh-related complications. In the present study, we prepared new kinds of meshes based on bacterial cellulose (BC) and collagen-coated BC (BCCOL) using a laser cutting method and perforation technique. The mechanical properties of pre-implanted BC meshes, including breaking strength, suture strength and rigidity, were equal to or exceeded those of available clinically used polypropylene meshes. An in vitro cellular assay revealed that BCCOL meshes exhibited enhanced biocompatibility by increasing collagen secretion and cell adhesion. Both BC and BCCOL meshes only caused weak inflammation and were surrounded by newly formed connective tissue composed of type I collagen after implantation in a rabbit subcutaneous model for one week, demonstrating that the novel mesh is fully biocompatible and can integrate into surrounding tissues. Furthermore, a long-term (ninety days) ewe vaginal implantation model was used to evaluate foreign body reactions and suitability of BC and BCCOL meshes as vaginal meshes. The results showed that the tissue surrounding the BC meshes returned to its original physiology as muscle tissue, indicating the excellent integration of BC meshes into the surrounding tissues without triggering severe local inflammatory response post-implantation. The collagen coating appeared to induce a chronic inflammatory response due to glutaraldehyde remnants. The present exploratory research demonstrated that the developed BC mesh might be a suitable candidate for treating POP.
format	article
author	Chen Lai Shu-Jiang Zhang Xuan-Chen Chen Li-Yuan Sheng Tian-Wei Qi Le-Ping Yan
author_facet	Chen Lai Shu-Jiang Zhang Xuan-Chen Chen Li-Yuan Sheng Tian-Wei Qi Le-Ping Yan
author_sort	Chen Lai
title	Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
title_short	Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
title_full	Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
title_fullStr	Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
title_full_unstemmed	Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
title_sort	development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: in vivo long-term evaluation in an ewe vagina model
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/dd70ef67c1164f659848ef8af380dc66
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Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model

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