Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model

The use of vaginal surgical mesh to treat pelvic organ prolapse (POP) has been associated with high rates of mesh-related complications. In the present study, we prepared new kinds of meshes based on bacterial cellulose (BC) and collagen-coated BC (BCCOL) using a laser cutting method and perforation...

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Autores principales: Chen Lai, Shu-Jiang Zhang, Xuan-Chen Chen, Li-Yuan Sheng, Tian-Wei Qi, Le-Ping Yan
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Lenguaje:EN
Publicado: Elsevier 2021
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spelling oai:doaj.org-article:dd70ef67c1164f659848ef8af380dc662021-12-02T05:03:34ZDevelopment of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model2590-006410.1016/j.mtbio.2021.100172https://doaj.org/article/dd70ef67c1164f659848ef8af380dc662021-09-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590006421000806https://doaj.org/toc/2590-0064The use of vaginal surgical mesh to treat pelvic organ prolapse (POP) has been associated with high rates of mesh-related complications. In the present study, we prepared new kinds of meshes based on bacterial cellulose (BC) and collagen-coated BC (BCCOL) using a laser cutting method and perforation technique. The mechanical properties of pre-implanted BC meshes, including breaking strength, suture strength and rigidity, were equal to or exceeded those of available clinically used polypropylene meshes. An in vitro cellular assay revealed that BCCOL meshes exhibited enhanced biocompatibility by increasing collagen secretion and cell adhesion. Both BC and BCCOL meshes only caused weak inflammation and were surrounded by newly formed connective tissue composed of type I collagen after implantation in a rabbit subcutaneous model for one week, demonstrating that the novel mesh is fully biocompatible and can integrate into surrounding tissues. Furthermore, a long-term (ninety days) ewe vaginal implantation model was used to evaluate foreign body reactions and suitability of BC and BCCOL meshes as vaginal meshes. The results showed that the tissue surrounding the BC meshes returned to its original physiology as muscle tissue, indicating the excellent integration of BC meshes into the surrounding tissues without triggering severe local inflammatory response post-implantation. The collagen coating appeared to induce a chronic inflammatory response due to glutaraldehyde remnants. The present exploratory research demonstrated that the developed BC mesh might be a suitable candidate for treating POP.Chen LaiShu-Jiang ZhangXuan-Chen ChenLi-Yuan ShengTian-Wei QiLe-Ping YanElsevierarticlePelvic organ prolapseCellulose-based vaginal meshIn vivo evaluationTissue integrationInflammatory reactionMedicine (General)R5-920Biology (General)QH301-705.5ENMaterials Today Bio, Vol 12, Iss , Pp 100172- (2021)
institution DOAJ
collection DOAJ
language EN
topic Pelvic organ prolapse
Cellulose-based vaginal mesh
In vivo evaluation
Tissue integration
Inflammatory reaction
Medicine (General)
R5-920
Biology (General)
QH301-705.5
spellingShingle Pelvic organ prolapse
Cellulose-based vaginal mesh
In vivo evaluation
Tissue integration
Inflammatory reaction
Medicine (General)
R5-920
Biology (General)
QH301-705.5
Chen Lai
Shu-Jiang Zhang
Xuan-Chen Chen
Li-Yuan Sheng
Tian-Wei Qi
Le-Ping Yan
Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
description The use of vaginal surgical mesh to treat pelvic organ prolapse (POP) has been associated with high rates of mesh-related complications. In the present study, we prepared new kinds of meshes based on bacterial cellulose (BC) and collagen-coated BC (BCCOL) using a laser cutting method and perforation technique. The mechanical properties of pre-implanted BC meshes, including breaking strength, suture strength and rigidity, were equal to or exceeded those of available clinically used polypropylene meshes. An in vitro cellular assay revealed that BCCOL meshes exhibited enhanced biocompatibility by increasing collagen secretion and cell adhesion. Both BC and BCCOL meshes only caused weak inflammation and were surrounded by newly formed connective tissue composed of type I collagen after implantation in a rabbit subcutaneous model for one week, demonstrating that the novel mesh is fully biocompatible and can integrate into surrounding tissues. Furthermore, a long-term (ninety days) ewe vaginal implantation model was used to evaluate foreign body reactions and suitability of BC and BCCOL meshes as vaginal meshes. The results showed that the tissue surrounding the BC meshes returned to its original physiology as muscle tissue, indicating the excellent integration of BC meshes into the surrounding tissues without triggering severe local inflammatory response post-implantation. The collagen coating appeared to induce a chronic inflammatory response due to glutaraldehyde remnants. The present exploratory research demonstrated that the developed BC mesh might be a suitable candidate for treating POP.
format article
author Chen Lai
Shu-Jiang Zhang
Xuan-Chen Chen
Li-Yuan Sheng
Tian-Wei Qi
Le-Ping Yan
author_facet Chen Lai
Shu-Jiang Zhang
Xuan-Chen Chen
Li-Yuan Sheng
Tian-Wei Qi
Le-Ping Yan
author_sort Chen Lai
title Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
title_short Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
title_full Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
title_fullStr Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
title_full_unstemmed Development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: In vivo long-term evaluation in an ewe vagina model
title_sort development of a cellulose-based prosthetic mesh for pelvic organ prolapse treatment: in vivo long-term evaluation in an ewe vagina model
publisher Elsevier
publishDate 2021
url https://doaj.org/article/dd70ef67c1164f659848ef8af380dc66
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