A novel role for vaping in mitochondrial gene dysregulation and inflammation fundamental to disease development

Abstract We constructed and analyzed the whole transcriptome in leukocytes of healthy adult vapers (with/without a history of smoking), ‘exclusive’ cigarette smokers, and controls (non-users of any tobacco products). Furthermore, we performed single-gene validation of expression data, and biochemica...

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Autores principales: Stella Tommasi, Niccolo Pabustan, Meng Li, Yibu Chen, Kimberly D. Siegmund, Ahmad Besaratinia
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/dd72f671dbf64e5bb3287a2fd5e0e489
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spelling oai:doaj.org-article:dd72f671dbf64e5bb3287a2fd5e0e4892021-11-28T12:18:36ZA novel role for vaping in mitochondrial gene dysregulation and inflammation fundamental to disease development10.1038/s41598-021-01965-12045-2322https://doaj.org/article/dd72f671dbf64e5bb3287a2fd5e0e4892021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01965-1https://doaj.org/toc/2045-2322Abstract We constructed and analyzed the whole transcriptome in leukocytes of healthy adult vapers (with/without a history of smoking), ‘exclusive’ cigarette smokers, and controls (non-users of any tobacco products). Furthermore, we performed single-gene validation of expression data, and biochemical validation of vaping/smoking status by plasma cotinine measurement. Computational modeling, combining primary analysis (age- and sex-adjusted limmaVoom) and sensitivity analysis (cumulative e-liquid- and pack-year modeling), revealed that ‘current’ vaping, but not ‘past’ smoking, is significantly associated with gene dysregulation in vapers. Comparative analysis of the gene networks and canonical pathways dysregulated in vapers and smokers showed strikingly similar patterns in the two groups, although the extent of transcriptomic changes was more pronounced in smokers than vapers. Of significance is the preferential targeting of mitochondrial genes in both vapers and smokers, concurrent with impaired functional networks, which drive mitochondrial DNA-related disorders. Equally significant is the dysregulation of immune response genes in vapers and smokers, modulated by upstream cytokines, including members of the interleukin and interferon family, which play a crucial role in inflammation. Our findings accord with the growing evidence on the central role of mitochondria as signaling organelles involved in immunity and inflammatory response, which are fundamental to disease development.Stella TommasiNiccolo PabustanMeng LiYibu ChenKimberly D. SiegmundAhmad BesaratiniaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Stella Tommasi
Niccolo Pabustan
Meng Li
Yibu Chen
Kimberly D. Siegmund
Ahmad Besaratinia
A novel role for vaping in mitochondrial gene dysregulation and inflammation fundamental to disease development
description Abstract We constructed and analyzed the whole transcriptome in leukocytes of healthy adult vapers (with/without a history of smoking), ‘exclusive’ cigarette smokers, and controls (non-users of any tobacco products). Furthermore, we performed single-gene validation of expression data, and biochemical validation of vaping/smoking status by plasma cotinine measurement. Computational modeling, combining primary analysis (age- and sex-adjusted limmaVoom) and sensitivity analysis (cumulative e-liquid- and pack-year modeling), revealed that ‘current’ vaping, but not ‘past’ smoking, is significantly associated with gene dysregulation in vapers. Comparative analysis of the gene networks and canonical pathways dysregulated in vapers and smokers showed strikingly similar patterns in the two groups, although the extent of transcriptomic changes was more pronounced in smokers than vapers. Of significance is the preferential targeting of mitochondrial genes in both vapers and smokers, concurrent with impaired functional networks, which drive mitochondrial DNA-related disorders. Equally significant is the dysregulation of immune response genes in vapers and smokers, modulated by upstream cytokines, including members of the interleukin and interferon family, which play a crucial role in inflammation. Our findings accord with the growing evidence on the central role of mitochondria as signaling organelles involved in immunity and inflammatory response, which are fundamental to disease development.
format article
author Stella Tommasi
Niccolo Pabustan
Meng Li
Yibu Chen
Kimberly D. Siegmund
Ahmad Besaratinia
author_facet Stella Tommasi
Niccolo Pabustan
Meng Li
Yibu Chen
Kimberly D. Siegmund
Ahmad Besaratinia
author_sort Stella Tommasi
title A novel role for vaping in mitochondrial gene dysregulation and inflammation fundamental to disease development
title_short A novel role for vaping in mitochondrial gene dysregulation and inflammation fundamental to disease development
title_full A novel role for vaping in mitochondrial gene dysregulation and inflammation fundamental to disease development
title_fullStr A novel role for vaping in mitochondrial gene dysregulation and inflammation fundamental to disease development
title_full_unstemmed A novel role for vaping in mitochondrial gene dysregulation and inflammation fundamental to disease development
title_sort novel role for vaping in mitochondrial gene dysregulation and inflammation fundamental to disease development
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/dd72f671dbf64e5bb3287a2fd5e0e489
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