Enhanced and sustained topical ocular delivery of cyclosporine A in thermosensitive hyaluronic acid-based in situ forming microgels

Yijun Wu, Jing Yao, Jianping Zhou, Fatima Zohra Dahmani State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang, Nanjing, People’s Republic of China Abstract: For nearly a decade, thermoresponsive ophthalmic in situ gels have been recognized as an interesti...

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Autores principales:	Wu Y, Yao J, Zhou J, Dahmani FZ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dd86c4efcdbf4c82b0210b560d22e418
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spelling	oai:doaj.org-article:dd86c4efcdbf4c82b0210b560d22e4182021-12-02T07:22:52ZEnhanced and sustained topical ocular delivery of cyclosporine A in thermosensitive hyaluronic acid-based in situ forming microgels1176-91141178-2013https://doaj.org/article/dd86c4efcdbf4c82b0210b560d22e4182013-09-01T00:00:00Zhttp://www.dovepress.com/enhanced-and-sustained-topical-ocular-delivery-of-cyclosporine-a-in-th-a14429https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yijun Wu, Jing Yao, Jianping Zhou, Fatima Zohra Dahmani State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang, Nanjing, People’s Republic of China Abstract: For nearly a decade, thermoresponsive ophthalmic in situ gels have been recognized as an interesting and promising ocular topical delivery vehicle for lipophilic drugs. In this study, a series of thermosensitive copolymers, hyaluronic acid-g-poly(N-isopropylacrylamide) (HA-g-PNIPAAm), was synthesized, by coupling carboxylic end-capped PNIPAAm to aminated hyaluronic acid through amide bond linkages, and was used as a potential carrier for the topical ocular administration of cyclosporine A (CyA). The lower critical solution temperature of HA-g-PNIPAAm59 in aqueous solutions was measured as 32.7°C, which was not significantly affected by the polymer concentration. Moreover, HA-g-PNIPAAm59 microgels showed a high drug loading efficiency (73.92%) and a controlled release profile that are necessary for biomedical application. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) observations showed that HA-g-PNIPAAm microgels were spherical in shape with homogeneous size. Based on the result of the eye irritation test, the HA-g-PNIPAAm microgels formulation was shown to be safe and nonirritant for rabbit eyes. In addition, HA-g-PNIPAAm microgels achieved significantly higher CyA concentration levels in rabbit corneas (1455.8 ng/g of tissue) than both castor oil formulation and commercial CyA eye drops. Therefore, these newly described thermoresponsive HA-g-PNIPAAm microgels demonstrated attractive properties to serve as pharmaceutical delivery vehicles for a variety of ophthalmic applications. Keywords: thermosensitive microgels, ophthalmic drug delivery, hyaluronic acid, cyclosporine AWu YYao JZhou JDahmani FZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 3587-3601 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wu Y Yao J Zhou J Dahmani FZ Enhanced and sustained topical ocular delivery of cyclosporine A in thermosensitive hyaluronic acid-based in situ forming microgels
description	Yijun Wu, Jing Yao, Jianping Zhou, Fatima Zohra Dahmani State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang, Nanjing, People’s Republic of China Abstract: For nearly a decade, thermoresponsive ophthalmic in situ gels have been recognized as an interesting and promising ocular topical delivery vehicle for lipophilic drugs. In this study, a series of thermosensitive copolymers, hyaluronic acid-g-poly(N-isopropylacrylamide) (HA-g-PNIPAAm), was synthesized, by coupling carboxylic end-capped PNIPAAm to aminated hyaluronic acid through amide bond linkages, and was used as a potential carrier for the topical ocular administration of cyclosporine A (CyA). The lower critical solution temperature of HA-g-PNIPAAm59 in aqueous solutions was measured as 32.7°C, which was not significantly affected by the polymer concentration. Moreover, HA-g-PNIPAAm59 microgels showed a high drug loading efficiency (73.92%) and a controlled release profile that are necessary for biomedical application. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) observations showed that HA-g-PNIPAAm microgels were spherical in shape with homogeneous size. Based on the result of the eye irritation test, the HA-g-PNIPAAm microgels formulation was shown to be safe and nonirritant for rabbit eyes. In addition, HA-g-PNIPAAm microgels achieved significantly higher CyA concentration levels in rabbit corneas (1455.8 ng/g of tissue) than both castor oil formulation and commercial CyA eye drops. Therefore, these newly described thermoresponsive HA-g-PNIPAAm microgels demonstrated attractive properties to serve as pharmaceutical delivery vehicles for a variety of ophthalmic applications. Keywords: thermosensitive microgels, ophthalmic drug delivery, hyaluronic acid, cyclosporine A
format	article
author	Wu Y Yao J Zhou J Dahmani FZ
author_facet	Wu Y Yao J Zhou J Dahmani FZ
author_sort	Wu Y
title	Enhanced and sustained topical ocular delivery of cyclosporine A in thermosensitive hyaluronic acid-based in situ forming microgels
title_short	Enhanced and sustained topical ocular delivery of cyclosporine A in thermosensitive hyaluronic acid-based in situ forming microgels
title_full	Enhanced and sustained topical ocular delivery of cyclosporine A in thermosensitive hyaluronic acid-based in situ forming microgels
title_fullStr	Enhanced and sustained topical ocular delivery of cyclosporine A in thermosensitive hyaluronic acid-based in situ forming microgels
title_full_unstemmed	Enhanced and sustained topical ocular delivery of cyclosporine A in thermosensitive hyaluronic acid-based in situ forming microgels
title_sort	enhanced and sustained topical ocular delivery of cyclosporine a in thermosensitive hyaluronic acid-based in situ forming microgels
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/dd86c4efcdbf4c82b0210b560d22e418
work_keys_str_mv	AT wuy enhancedandsustainedtopicaloculardeliveryofcyclosporineainthermosensitivehyaluronicacidbasedinsituformingmicrogels AT yaoj enhancedandsustainedtopicaloculardeliveryofcyclosporineainthermosensitivehyaluronicacidbasedinsituformingmicrogels AT zhouj enhancedandsustainedtopicaloculardeliveryofcyclosporineainthermosensitivehyaluronicacidbasedinsituformingmicrogels AT dahmanifz enhancedandsustainedtopicaloculardeliveryofcyclosporineainthermosensitivehyaluronicacidbasedinsituformingmicrogels
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Enhanced and sustained topical ocular delivery of cyclosporine A in thermosensitive hyaluronic acid-based in situ forming microgels

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