Characterization of microRNAs expressed during secondary wall biosynthesis in Acacia mangium.

MicroRNAs (miRNAs) play critical regulatory roles by acting as sequence specific guide during secondary wall formation in woody and non-woody species. Although thousands of plant miRNAs have been sequenced, there is no comprehensive view of miRNA mediated gene regulatory network to provide profound...

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Autores principales:	Seong Siang Ong, Ratnam Wickneswari
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd87064d578043ed89a8fc6aabe17256
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spelling	oai:doaj.org-article:dd87064d578043ed89a8fc6aabe172562021-11-18T08:07:20ZCharacterization of microRNAs expressed during secondary wall biosynthesis in Acacia mangium.1932-620310.1371/journal.pone.0049662https://doaj.org/article/dd87064d578043ed89a8fc6aabe172562012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23251324/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs (miRNAs) play critical regulatory roles by acting as sequence specific guide during secondary wall formation in woody and non-woody species. Although thousands of plant miRNAs have been sequenced, there is no comprehensive view of miRNA mediated gene regulatory network to provide profound biological insights into the regulation of xylem development. Herein, we report the involvement of six highly conserved amg-miRNA families (amg-miR166, amg-miR172, amg-miR168, amg-miR159, amg-miR394, and amg-miR156) as the potential regulatory sequences of secondary cell wall biosynthesis. Within this highly conserved amg-miRNA family, only amg-miR166 exhibited strong differences in expression between phloem and xylem tissue. The functional characterization of amg-miR166 targets in various tissues revealed three groups of HD-ZIP III: ATHB8, ATHB15, and REVOLUTA which play pivotal roles in xylem development. Although these three groups vary in their functions, -psRNA target analysis indicated that miRNA target sequences of the nine different members of HD-ZIP III are always conserved. We found that precursor structures of amg-miR166 undergo exhaustive sequence variation even within members of the same family. Gene expression analysis showed three key lignin pathway genes: C4H, CAD, and CCoAOMT were upregulated in compression wood where a cascade of miRNAs was downregulated. This study offers a comprehensive analysis on the involvement of highly conserved miRNAs implicated in the secondary wall formation of woody plants.Seong Siang OngRatnam WickneswariPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49662 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seong Siang Ong Ratnam Wickneswari Characterization of microRNAs expressed during secondary wall biosynthesis in Acacia mangium.
description	MicroRNAs (miRNAs) play critical regulatory roles by acting as sequence specific guide during secondary wall formation in woody and non-woody species. Although thousands of plant miRNAs have been sequenced, there is no comprehensive view of miRNA mediated gene regulatory network to provide profound biological insights into the regulation of xylem development. Herein, we report the involvement of six highly conserved amg-miRNA families (amg-miR166, amg-miR172, amg-miR168, amg-miR159, amg-miR394, and amg-miR156) as the potential regulatory sequences of secondary cell wall biosynthesis. Within this highly conserved amg-miRNA family, only amg-miR166 exhibited strong differences in expression between phloem and xylem tissue. The functional characterization of amg-miR166 targets in various tissues revealed three groups of HD-ZIP III: ATHB8, ATHB15, and REVOLUTA which play pivotal roles in xylem development. Although these three groups vary in their functions, -psRNA target analysis indicated that miRNA target sequences of the nine different members of HD-ZIP III are always conserved. We found that precursor structures of amg-miR166 undergo exhaustive sequence variation even within members of the same family. Gene expression analysis showed three key lignin pathway genes: C4H, CAD, and CCoAOMT were upregulated in compression wood where a cascade of miRNAs was downregulated. This study offers a comprehensive analysis on the involvement of highly conserved miRNAs implicated in the secondary wall formation of woody plants.
format	article
author	Seong Siang Ong Ratnam Wickneswari
author_facet	Seong Siang Ong Ratnam Wickneswari
author_sort	Seong Siang Ong
title	Characterization of microRNAs expressed during secondary wall biosynthesis in Acacia mangium.
title_short	Characterization of microRNAs expressed during secondary wall biosynthesis in Acacia mangium.
title_full	Characterization of microRNAs expressed during secondary wall biosynthesis in Acacia mangium.
title_fullStr	Characterization of microRNAs expressed during secondary wall biosynthesis in Acacia mangium.
title_full_unstemmed	Characterization of microRNAs expressed during secondary wall biosynthesis in Acacia mangium.
title_sort	characterization of micrornas expressed during secondary wall biosynthesis in acacia mangium.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/dd87064d578043ed89a8fc6aabe17256
work_keys_str_mv	AT seongsiangong characterizationofmicrornasexpressedduringsecondarywallbiosynthesisinacaciamangium AT ratnamwickneswari characterizationofmicrornasexpressedduringsecondarywallbiosynthesisinacaciamangium
_version_	1718422200251318272

Characterization of microRNAs expressed during secondary wall biosynthesis in Acacia mangium.

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