SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro

Yang Xue, Qingqing Chen, Tingting Ding, Jiao SunShanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of ChinaAbstract: The liver has been s...

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Autores principales:	Xue Y, Chen Q, Ding T, Sun J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dd8ad344c7a947b89adb4545c9687263
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spelling	oai:doaj.org-article:dd8ad344c7a947b89adb4545c96872632021-12-02T03:05:14ZSiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro1178-2013https://doaj.org/article/dd8ad344c7a947b89adb4545c96872632014-06-01T00:00:00Zhttp://www.dovepress.com/sio2-nanoparticle-induced-impairment-of-mitochondrial-energy-metabolis-a17167https://doaj.org/toc/1178-2013 Yang Xue, Qingqing Chen, Tingting Ding, Jiao SunShanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of ChinaAbstract: The liver has been shown to be a primary target organ for SiO2 nanoparticles in vivo, and may be highly susceptible to damage by these nanoparticles. However, until now, research focusing on the potential toxic effects of SiO2 nanoparticles on mitochondria-associated energy metabolism in hepatocytes has been lacking. In this work, SiO2 nanoparticles 20 nm in diameter were evaluated for their ability to induce dysfunction of mitochondrial energy metabolism. First, a buffalo rat liver (BRL) cell line was directly exposed to SiO2 nanoparticles, which induced cytotoxicity and mitochondrial damage accompanied by decreases in mitochondrial dehydrogenase activity, mitochondrial membrane potential, enzymatic expression in the Krebs cycle, and activity of the mitochondrial respiratory chain complexes I, III and IV. Second, the role of rat-derived Kupffer cells was evaluated. The supernatants from Kupffer cells treated with SiO2 nanoparticles were transferred to stimulate BRL cells. We observed that SiO2 nanoparticles had the ability to activate Kupffer cells, leading to release of tumor necrosis factor-α, nitric oxide, and reactive oxygen species from these cells and subsequently to inhibition of mitochondrial respiratory chain complex I activity in BRL cells.Keywords: SiO2 nanoparticles, mitochondria-associated energy metabolism, buffalo rat liver cells, Kupffer cellsXue YChen QDing TSun JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2891-2903 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Xue Y Chen Q Ding T Sun J SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro
description	Yang Xue, Qingqing Chen, Tingting Ding, Jiao SunShanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of ChinaAbstract: The liver has been shown to be a primary target organ for SiO2 nanoparticles in vivo, and may be highly susceptible to damage by these nanoparticles. However, until now, research focusing on the potential toxic effects of SiO2 nanoparticles on mitochondria-associated energy metabolism in hepatocytes has been lacking. In this work, SiO2 nanoparticles 20 nm in diameter were evaluated for their ability to induce dysfunction of mitochondrial energy metabolism. First, a buffalo rat liver (BRL) cell line was directly exposed to SiO2 nanoparticles, which induced cytotoxicity and mitochondrial damage accompanied by decreases in mitochondrial dehydrogenase activity, mitochondrial membrane potential, enzymatic expression in the Krebs cycle, and activity of the mitochondrial respiratory chain complexes I, III and IV. Second, the role of rat-derived Kupffer cells was evaluated. The supernatants from Kupffer cells treated with SiO2 nanoparticles were transferred to stimulate BRL cells. We observed that SiO2 nanoparticles had the ability to activate Kupffer cells, leading to release of tumor necrosis factor-α, nitric oxide, and reactive oxygen species from these cells and subsequently to inhibition of mitochondrial respiratory chain complex I activity in BRL cells.Keywords: SiO2 nanoparticles, mitochondria-associated energy metabolism, buffalo rat liver cells, Kupffer cells
format	article
author	Xue Y Chen Q Ding T Sun J
author_facet	Xue Y Chen Q Ding T Sun J
author_sort	Xue Y
title	SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro
title_short	SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro
title_full	SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro
title_fullStr	SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro
title_full_unstemmed	SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro
title_sort	sio2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a kupffer cell-mediated pathway in vitro
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/dd8ad344c7a947b89adb4545c9687263
work_keys_str_mv	AT xuey sio2nanoparticleinducedimpairmentofmitochondrialenergymetabolisminhepatocytesdirectlyandthroughakupffercellmediatedpathwayinvitro AT chenq sio2nanoparticleinducedimpairmentofmitochondrialenergymetabolisminhepatocytesdirectlyandthroughakupffercellmediatedpathwayinvitro AT dingt sio2nanoparticleinducedimpairmentofmitochondrialenergymetabolisminhepatocytesdirectlyandthroughakupffercellmediatedpathwayinvitro AT sunj sio2nanoparticleinducedimpairmentofmitochondrialenergymetabolisminhepatocytesdirectlyandthroughakupffercellmediatedpathwayinvitro
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SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro

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