Biological Functions and Prognostic Value of Ferroptosis-Related Genes in Bladder Cancer

Background: Every year, nearly 170,000 people die from bladder cancer worldwide. A major problem after transurethral resection of bladder tumor is that 40–80% of the tumors recur. Ferroptosis is a type of regulatory necrosis mediated by iron-catalyzed, excessive oxidation of polyunsaturated fatty ac...

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Autores principales:	Kezhen Yi, JingChong Liu, Yuan Rong, Cheng Wang, Xuan Tang, XiaoPing Zhang, Yunhe Xiong, Fubing Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bladder cancer ferroptosis prognostic model construction survival analysis mRNA Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dd90a8db15814a4dadd0435f4cb596f7
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spelling	oai:doaj.org-article:dd90a8db15814a4dadd0435f4cb596f72021-11-17T07:05:02ZBiological Functions and Prognostic Value of Ferroptosis-Related Genes in Bladder Cancer2296-889X10.3389/fmolb.2021.631152https://doaj.org/article/dd90a8db15814a4dadd0435f4cb596f72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.631152/fullhttps://doaj.org/toc/2296-889XBackground: Every year, nearly 170,000 people die from bladder cancer worldwide. A major problem after transurethral resection of bladder tumor is that 40–80% of the tumors recur. Ferroptosis is a type of regulatory necrosis mediated by iron-catalyzed, excessive oxidation of polyunsaturated fatty acids. Increasing the sensitivity of tumor cells to ferroptosis is a potential treatment option for cancer. Establishing a diagnostic and prognostic model based on ferroptosis-related genes may provide guidance for the precise treatment of bladder cancer.Methods: We downloaded mRNA data in Bladder Cancer from The Cancer Genome Atlas and analyzed differentially expressed genes based on and extract ferroptosis-related genes. We identified relevant pathways and annotate the functions of ferroptosis-related DEGs using Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and Gene Ontology functions. On the website of Search Tool for Retrieving Interacting Genes database (STRING), we downloaded the protein-protein interactions of DEGs, which were drawn by the Cytoscape software. Then the Cox regression analysis were performed so that the prognostic value of ferroptosis-related genes and survival time are combined to identify survival- and ferroptosis-related genes and establish a prognostic formula. Survival analysis and receiver operating characteristic curvevalidation were then performed. Risk curves and nomograms were generated for both groups to predict survival. Finally, RT-qPCR was applied to analyze gene expression.Results: Eight ferroptosis-related genes with prognostic value (ISCU, NFE2L2, MAFG, ZEB1, VDAC2, TXNIP, SCD, and JDP2) were identified. With clinical data, we established a prognostic model to provide promising diagnostic and prognostic information of bladder cancer based on the eight ferroptosis-related genes. RT-qPCR revealed the genes that were differentially expressed between normal and cancer tissues.Conclusion: This study found that the ferroptosis-related genes is associated with bladder cancer, which may serve as new target for the treatment of bladder cancer.Kezhen YiJingChong LiuJingChong LiuYuan RongCheng WangXuan TangXiaoPing ZhangXiaoPing ZhangYunhe XiongFubing WangFubing WangFubing WangFrontiers Media S.A.articlebladder cancerferroptosisprognostic model constructionsurvival analysismRNABiology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bladder cancer ferroptosis prognostic model construction survival analysis mRNA Biology (General) QH301-705.5
spellingShingle	bladder cancer ferroptosis prognostic model construction survival analysis mRNA Biology (General) QH301-705.5 Kezhen Yi JingChong Liu JingChong Liu Yuan Rong Cheng Wang Xuan Tang XiaoPing Zhang XiaoPing Zhang Yunhe Xiong Fubing Wang Fubing Wang Fubing Wang Biological Functions and Prognostic Value of Ferroptosis-Related Genes in Bladder Cancer
description	Background: Every year, nearly 170,000 people die from bladder cancer worldwide. A major problem after transurethral resection of bladder tumor is that 40–80% of the tumors recur. Ferroptosis is a type of regulatory necrosis mediated by iron-catalyzed, excessive oxidation of polyunsaturated fatty acids. Increasing the sensitivity of tumor cells to ferroptosis is a potential treatment option for cancer. Establishing a diagnostic and prognostic model based on ferroptosis-related genes may provide guidance for the precise treatment of bladder cancer.Methods: We downloaded mRNA data in Bladder Cancer from The Cancer Genome Atlas and analyzed differentially expressed genes based on and extract ferroptosis-related genes. We identified relevant pathways and annotate the functions of ferroptosis-related DEGs using Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and Gene Ontology functions. On the website of Search Tool for Retrieving Interacting Genes database (STRING), we downloaded the protein-protein interactions of DEGs, which were drawn by the Cytoscape software. Then the Cox regression analysis were performed so that the prognostic value of ferroptosis-related genes and survival time are combined to identify survival- and ferroptosis-related genes and establish a prognostic formula. Survival analysis and receiver operating characteristic curvevalidation were then performed. Risk curves and nomograms were generated for both groups to predict survival. Finally, RT-qPCR was applied to analyze gene expression.Results: Eight ferroptosis-related genes with prognostic value (ISCU, NFE2L2, MAFG, ZEB1, VDAC2, TXNIP, SCD, and JDP2) were identified. With clinical data, we established a prognostic model to provide promising diagnostic and prognostic information of bladder cancer based on the eight ferroptosis-related genes. RT-qPCR revealed the genes that were differentially expressed between normal and cancer tissues.Conclusion: This study found that the ferroptosis-related genes is associated with bladder cancer, which may serve as new target for the treatment of bladder cancer.
format	article
author	Kezhen Yi JingChong Liu JingChong Liu Yuan Rong Cheng Wang Xuan Tang XiaoPing Zhang XiaoPing Zhang Yunhe Xiong Fubing Wang Fubing Wang Fubing Wang
author_facet	Kezhen Yi JingChong Liu JingChong Liu Yuan Rong Cheng Wang Xuan Tang XiaoPing Zhang XiaoPing Zhang Yunhe Xiong Fubing Wang Fubing Wang Fubing Wang
author_sort	Kezhen Yi
title	Biological Functions and Prognostic Value of Ferroptosis-Related Genes in Bladder Cancer
title_short	Biological Functions and Prognostic Value of Ferroptosis-Related Genes in Bladder Cancer
title_full	Biological Functions and Prognostic Value of Ferroptosis-Related Genes in Bladder Cancer
title_fullStr	Biological Functions and Prognostic Value of Ferroptosis-Related Genes in Bladder Cancer
title_full_unstemmed	Biological Functions and Prognostic Value of Ferroptosis-Related Genes in Bladder Cancer
title_sort	biological functions and prognostic value of ferroptosis-related genes in bladder cancer
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/dd90a8db15814a4dadd0435f4cb596f7
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_version_	1718425894295437312

Biological Functions and Prognostic Value of Ferroptosis-Related Genes in Bladder Cancer

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