easyCLIP analysis of RNA-protein interactions incorporating absolute quantification

It is important to develop quantitative criteria to identify RNA-binding proteins and target RNAs. Here, the authors present a method to quantify cross-linking and apply it to recurrent cancer mutations in RNA-binding proteins.

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Detalles Bibliográficos
Autores principales: Douglas F. Porter, Weili Miao, Xue Yang, Grant A. Goda, Andrew L. Ji, Laura K. H. Donohue, Maria M. Aleman, Daniel Dominguez, Paul A. Khavari
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/dd96eec6ff1a4e01a51eb5bbd511fef7
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Sumario:It is important to develop quantitative criteria to identify RNA-binding proteins and target RNAs. Here, the authors present a method to quantify cross-linking and apply it to recurrent cancer mutations in RNA-binding proteins.