Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases

This work introduces a novel way to obtain catalytically competent oxyferryl species for two different dye-decolorizing peroxidases (DyPs) in the absence of H<sub>2</sub>O<sub>2</sub> or any other peroxide by simply applying a reductive electrochemical potential under aerobic...

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Autores principales:	Magalí F. Scocozza, Lígia O. Martins, Daniel H. Murgida
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dye-decolorizing peroxidases compound I compound II Raman spectroscopy spectroelectrochemistry Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/dd972741cbe34ac985f49ef4c528795d
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spelling	oai:doaj.org-article:dd972741cbe34ac985f49ef4c528795d2021-11-25T17:57:33ZDirect Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases10.3390/ijms2222125321422-00671661-6596https://doaj.org/article/dd972741cbe34ac985f49ef4c528795d2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12532https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067This work introduces a novel way to obtain catalytically competent oxyferryl species for two different dye-decolorizing peroxidases (DyPs) in the absence of H<sub>2</sub>O<sub>2</sub> or any other peroxide by simply applying a reductive electrochemical potential under aerobic conditions. UV-vis and resonance Raman spectroscopies show that this method yields long-lived compounds II and I for the DyPs from <i>Bacillus subtilis</i> (BsDyP; Class I) and <i>Pseudomonas putida</i> (PpDyP; Class P), respectively. Both electrochemically generated high valent intermediates are able to oxidize ABTS at both acidic and alkaline pH. Interestingly, the electrocatalytic efficiencies obtained at pH 7.6 are very similar to the values recorded for regular catalytic ABTS/H<sub>2</sub>O<sub>2</sub> assays at the optimal pH of the enzymes, ca. 3.7. These findings pave the way for the design of DyP-based electrocatalytic reactors operable in an extended pH range without the need of harmful reagents such as H<sub>2</sub>O<sub>2</sub>.Magalí F. ScocozzaLígia O. MartinsDaniel H. MurgidaMDPI AGarticledye-decolorizing peroxidasescompound Icompound IIRaman spectroscopyspectroelectrochemistryBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12532, p 12532 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dye-decolorizing peroxidases compound I compound II Raman spectroscopy spectroelectrochemistry Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	dye-decolorizing peroxidases compound I compound II Raman spectroscopy spectroelectrochemistry Biology (General) QH301-705.5 Chemistry QD1-999 Magalí F. Scocozza Lígia O. Martins Daniel H. Murgida Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases
description	This work introduces a novel way to obtain catalytically competent oxyferryl species for two different dye-decolorizing peroxidases (DyPs) in the absence of H<sub>2</sub>O<sub>2</sub> or any other peroxide by simply applying a reductive electrochemical potential under aerobic conditions. UV-vis and resonance Raman spectroscopies show that this method yields long-lived compounds II and I for the DyPs from <i>Bacillus subtilis</i> (BsDyP; Class I) and <i>Pseudomonas putida</i> (PpDyP; Class P), respectively. Both electrochemically generated high valent intermediates are able to oxidize ABTS at both acidic and alkaline pH. Interestingly, the electrocatalytic efficiencies obtained at pH 7.6 are very similar to the values recorded for regular catalytic ABTS/H<sub>2</sub>O<sub>2</sub> assays at the optimal pH of the enzymes, ca. 3.7. These findings pave the way for the design of DyP-based electrocatalytic reactors operable in an extended pH range without the need of harmful reagents such as H<sub>2</sub>O<sub>2</sub>.
format	article
author	Magalí F. Scocozza Lígia O. Martins Daniel H. Murgida
author_facet	Magalí F. Scocozza Lígia O. Martins Daniel H. Murgida
author_sort	Magalí F. Scocozza
title	Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases
title_short	Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases
title_full	Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases
title_fullStr	Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases
title_full_unstemmed	Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases
title_sort	direct electrochemical generation of catalytically competent oxyferryl species of classes i and p dye decolorizing peroxidases
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dd972741cbe34ac985f49ef4c528795d
work_keys_str_mv	AT magalifscocozza directelectrochemicalgenerationofcatalyticallycompetentoxyferrylspeciesofclassesiandpdyedecolorizingperoxidases AT ligiaomartins directelectrochemicalgenerationofcatalyticallycompetentoxyferrylspeciesofclassesiandpdyedecolorizingperoxidases AT danielhmurgida directelectrochemicalgenerationofcatalyticallycompetentoxyferrylspeciesofclassesiandpdyedecolorizingperoxidases
_version_	1718411835908030464

Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases

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