Temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?

Although the ecological importance of coinfection is increasingly recognized, analyses of microbial pathogen dynamics in wildlife usually focus on an ad hoc subset of the species present due to technological limitations on detection. Here we demonstrate the use of expression profiles for immunologic...

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Autores principales:	Ida M Friberg, Ann Lowe, Catriona Ralli, Janette E Bradley, Joseph A Jackson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dd9e084862d24424bbfbac89d3ec7ba9
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spelling	oai:doaj.org-article:dd9e084862d24424bbfbac89d3ec7ba92021-11-18T06:53:27ZTemporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?1932-620310.1371/journal.pone.0020070https://doaj.org/article/dd9e084862d24424bbfbac89d3ec7ba92011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21629775/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Although the ecological importance of coinfection is increasingly recognized, analyses of microbial pathogen dynamics in wildlife usually focus on an ad hoc subset of the species present due to technological limitations on detection. Here we demonstrate the use of expression profiles for immunological genes (pattern recognition receptors, cytokines and transcription factors) as a means to identify, without preconception, the likelihood of important acute microbial infections in wildlife. Using a wood mouse population in the UK as a model we identified significant temporal clusters of individuals with extreme expression of immunological mediators across multiple loci, typical of an acute microbial infection. These clusters were circumstantially associated with demographic perturbation in the summertime wood mouse population. Animals in one cluster also had significantly higher individual macroparasite burdens than contemporaries with "normal" expression patterns. If the extreme transcriptional profiles observed are induced by an infectious agent then this implicates macroparasites as a possible player in mediating individual susceptibility or resilience to infection. The form of survey described here, combined with next generation nucleic acids sequencing methods for the broad detection of microbial infectious agents in individuals with anomalous immunological transcriptional profiles, could be a powerful tool for revealing unrecognized, ecologically important infectious agents circulating in wildlife populations.Ida M FribergAnn LoweCatriona RalliJanette E BradleyJoseph A JacksonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20070 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ida M Friberg Ann Lowe Catriona Ralli Janette E Bradley Joseph A Jackson Temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?
description	Although the ecological importance of coinfection is increasingly recognized, analyses of microbial pathogen dynamics in wildlife usually focus on an ad hoc subset of the species present due to technological limitations on detection. Here we demonstrate the use of expression profiles for immunological genes (pattern recognition receptors, cytokines and transcription factors) as a means to identify, without preconception, the likelihood of important acute microbial infections in wildlife. Using a wood mouse population in the UK as a model we identified significant temporal clusters of individuals with extreme expression of immunological mediators across multiple loci, typical of an acute microbial infection. These clusters were circumstantially associated with demographic perturbation in the summertime wood mouse population. Animals in one cluster also had significantly higher individual macroparasite burdens than contemporaries with "normal" expression patterns. If the extreme transcriptional profiles observed are induced by an infectious agent then this implicates macroparasites as a possible player in mediating individual susceptibility or resilience to infection. The form of survey described here, combined with next generation nucleic acids sequencing methods for the broad detection of microbial infectious agents in individuals with anomalous immunological transcriptional profiles, could be a powerful tool for revealing unrecognized, ecologically important infectious agents circulating in wildlife populations.
format	article
author	Ida M Friberg Ann Lowe Catriona Ralli Janette E Bradley Joseph A Jackson
author_facet	Ida M Friberg Ann Lowe Catriona Ralli Janette E Bradley Joseph A Jackson
author_sort	Ida M Friberg
title	Temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?
title_short	Temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?
title_full	Temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?
title_fullStr	Temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?
title_full_unstemmed	Temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?
title_sort	temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/dd9e084862d24424bbfbac89d3ec7ba9
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_version_	1718424260120150016

Temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?

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