Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus

Abstract Anaeroglobus geminatus is a relatively newly discovered putative pathogen, with a potential role in the microbial shift associated with periodontitis, a disease that causes inflammatory destruction of the periodontal tissues, and eventually tooth loss. This study aimed to introduce A. gemin...

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Autores principales:	Kai Bao, Nagihan Bostanci, Thomas Thurnheer, Georgios N. Belibasakis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ddab90fb95be4bb88367b1b34c41619d
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spelling	oai:doaj.org-article:ddab90fb95be4bb88367b1b34c41619d2021-12-02T11:52:16ZProteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus10.1038/s41598-017-04594-92045-2322https://doaj.org/article/ddab90fb95be4bb88367b1b34c41619d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04594-9https://doaj.org/toc/2045-2322Abstract Anaeroglobus geminatus is a relatively newly discovered putative pathogen, with a potential role in the microbial shift associated with periodontitis, a disease that causes inflammatory destruction of the periodontal tissues, and eventually tooth loss. This study aimed to introduce A. geminatus into a polymicrobial biofilm model of relevance to periodontitis, and monitor the proteomic responses exerted to the rest of the biofilm community. A. geminatus was grown together with another 10-species in a well-established “subgingival” in vitro biofilm model. Its effects on the other species were quantitatively evaluated by qPCR and label-free proteomics. A. geminatus caused a significant increase in P. intermedia numbers, but not the other species in the biofilm. Whole cell proteome profiling of the biofilms by LC-MS/MS identified a total of 3213 proteins. Label-free quantitative proteomics revealed that 187 proteins belonging to the other 10 species were differentially abundant when A. geminatus was present in the biofilm. The species with most up-regulated and down-regulated proteins were P. intermedia and S. oralis, respectively. Regulated proteins were of primarily of ribosomal origin, and other affected categories involved proteolysis, carbon metabolism and iron transport. In conclusion, A. geminatus can be successfully grown in a polymicrobial biofilm community, causing quantitative proteomic shifts commensurate with increased virulence properties.Kai BaoNagihan BostanciThomas ThurnheerGeorgios N. BelibasakisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Bao Nagihan Bostanci Thomas Thurnheer Georgios N. Belibasakis Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus
description	Abstract Anaeroglobus geminatus is a relatively newly discovered putative pathogen, with a potential role in the microbial shift associated with periodontitis, a disease that causes inflammatory destruction of the periodontal tissues, and eventually tooth loss. This study aimed to introduce A. geminatus into a polymicrobial biofilm model of relevance to periodontitis, and monitor the proteomic responses exerted to the rest of the biofilm community. A. geminatus was grown together with another 10-species in a well-established “subgingival” in vitro biofilm model. Its effects on the other species were quantitatively evaluated by qPCR and label-free proteomics. A. geminatus caused a significant increase in P. intermedia numbers, but not the other species in the biofilm. Whole cell proteome profiling of the biofilms by LC-MS/MS identified a total of 3213 proteins. Label-free quantitative proteomics revealed that 187 proteins belonging to the other 10 species were differentially abundant when A. geminatus was present in the biofilm. The species with most up-regulated and down-regulated proteins were P. intermedia and S. oralis, respectively. Regulated proteins were of primarily of ribosomal origin, and other affected categories involved proteolysis, carbon metabolism and iron transport. In conclusion, A. geminatus can be successfully grown in a polymicrobial biofilm community, causing quantitative proteomic shifts commensurate with increased virulence properties.
format	article
author	Kai Bao Nagihan Bostanci Thomas Thurnheer Georgios N. Belibasakis
author_facet	Kai Bao Nagihan Bostanci Thomas Thurnheer Georgios N. Belibasakis
author_sort	Kai Bao
title	Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus
title_short	Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus
title_full	Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus
title_fullStr	Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus
title_full_unstemmed	Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus
title_sort	proteomic shifts in multi-species oral biofilms caused by anaeroglobus geminatus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ddab90fb95be4bb88367b1b34c41619d
work_keys_str_mv	AT kaibao proteomicshiftsinmultispeciesoralbiofilmscausedbyanaeroglobusgeminatus AT nagihanbostanci proteomicshiftsinmultispeciesoralbiofilmscausedbyanaeroglobusgeminatus AT thomasthurnheer proteomicshiftsinmultispeciesoralbiofilmscausedbyanaeroglobusgeminatus AT georgiosnbelibasakis proteomicshiftsinmultispeciesoralbiofilmscausedbyanaeroglobusgeminatus
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Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus

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