A new in vitro model applied 90Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions
Abstract We propose a new in vitro model to assess the impact of 90Y-microspheres derived low-dose beta radiation on colorectal cancer cell line under various oxygenation conditions that mimic the tumor environment. Cancer cells (HCT116) proliferation was assessed using Alamar Blue (AB) assay after...
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Nature Portfolio
2021
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oai:doaj.org-article:ddb131cc826e415588622fc803fb9af52021-12-02T11:35:53ZA new in vitro model applied 90Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions10.1038/s41598-021-84000-72045-2322https://doaj.org/article/ddb131cc826e415588622fc803fb9af52021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84000-7https://doaj.org/toc/2045-2322Abstract We propose a new in vitro model to assess the impact of 90Y-microspheres derived low-dose beta radiation on colorectal cancer cell line under various oxygenation conditions that mimic the tumor environment. Cancer cells (HCT116) proliferation was assessed using Alamar Blue (AB) assay after 48, 72, and 96 h. FLUKA code assessed changes in cancer cell populations relative to the absorbed dose. In normoxia, mitochondrial activity measured by Alamar Blue after 48–72 h was significantly correlated with the number of microspheres (48 h: r = 0.87 and 72 h: r = 0.89, p < 0.05) and absorbed dose (48 h: r = 0.87 and 72 h: r = 0.7, p < 0.05). In hypoxia, the coefficients were r = 0.43 for both the number of spheres and absorbed dose and r = 0.45, r = 0.47, respectively. Impediment of cancer cell proliferation depended on the absorbed dose. Doses below 70 Gy could reduce colorectal cancer cell proliferation in vitro. Hypoxia induced a higher resistance to radiation than that observed under normoxic conditions. Hypoxia and radiation induced senescence in cultured cells. The new in vitro model is useful for the assessment of 90Y radioembolization effects at the micro-scale.Piotr PiaseckiAleksandra MajewskaJerzy NarlochMaciej MaciakKlaudia BrodaczewskaMichal KucHalina WasMarek WierzbickiKrzysztof BrzozowskiPiotr ZiecinaAndrzej MazurekMiroslaw DziukEdward IllerClaudine KiedaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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Medicine R Science Q Piotr Piasecki Aleksandra Majewska Jerzy Narloch Maciej Maciak Klaudia Brodaczewska Michal Kuc Halina Was Marek Wierzbicki Krzysztof Brzozowski Piotr Ziecina Andrzej Mazurek Miroslaw Dziuk Edward Iller Claudine Kieda A new in vitro model applied 90Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions |
| description |
Abstract We propose a new in vitro model to assess the impact of 90Y-microspheres derived low-dose beta radiation on colorectal cancer cell line under various oxygenation conditions that mimic the tumor environment. Cancer cells (HCT116) proliferation was assessed using Alamar Blue (AB) assay after 48, 72, and 96 h. FLUKA code assessed changes in cancer cell populations relative to the absorbed dose. In normoxia, mitochondrial activity measured by Alamar Blue after 48–72 h was significantly correlated with the number of microspheres (48 h: r = 0.87 and 72 h: r = 0.89, p < 0.05) and absorbed dose (48 h: r = 0.87 and 72 h: r = 0.7, p < 0.05). In hypoxia, the coefficients were r = 0.43 for both the number of spheres and absorbed dose and r = 0.45, r = 0.47, respectively. Impediment of cancer cell proliferation depended on the absorbed dose. Doses below 70 Gy could reduce colorectal cancer cell proliferation in vitro. Hypoxia induced a higher resistance to radiation than that observed under normoxic conditions. Hypoxia and radiation induced senescence in cultured cells. The new in vitro model is useful for the assessment of 90Y radioembolization effects at the micro-scale. |
| format |
article |
| author |
Piotr Piasecki Aleksandra Majewska Jerzy Narloch Maciej Maciak Klaudia Brodaczewska Michal Kuc Halina Was Marek Wierzbicki Krzysztof Brzozowski Piotr Ziecina Andrzej Mazurek Miroslaw Dziuk Edward Iller Claudine Kieda |
| author_facet |
Piotr Piasecki Aleksandra Majewska Jerzy Narloch Maciej Maciak Klaudia Brodaczewska Michal Kuc Halina Was Marek Wierzbicki Krzysztof Brzozowski Piotr Ziecina Andrzej Mazurek Miroslaw Dziuk Edward Iller Claudine Kieda |
| author_sort |
Piotr Piasecki |
| title |
A new in vitro model applied 90Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions |
| title_short |
A new in vitro model applied 90Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions |
| title_full |
A new in vitro model applied 90Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions |
| title_fullStr |
A new in vitro model applied 90Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions |
| title_full_unstemmed |
A new in vitro model applied 90Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions |
| title_sort |
new in vitro model applied 90y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/ddb131cc826e415588622fc803fb9af5 |
| work_keys_str_mv |
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