Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids

Abstract Azadirachta indica A. Juss, commonly known as Neem, is the reservoir of triterpenoids of economic importance. Metabolite analysis of different developmental stages of leaf and fruit suggests tissue-specific accumulation of the major triterpenoids in this important tree. Though biosynthesis...

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Autores principales:	Sweta Bhambhani, Deepika Lakhwani, Parul Gupta, Ashutosh Pandey, Yogeshwar Vikram Dhar, Sumit Kumar Bag, Mehar Hasan Asif, Prabodh Kumar Trivedi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ddbe79eb7bd24d53b9b50da2a4e82ea2
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spelling	oai:doaj.org-article:ddbe79eb7bd24d53b9b50da2a4e82ea22021-12-02T16:08:19ZTranscriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids10.1038/s41598-017-05291-32045-2322https://doaj.org/article/ddbe79eb7bd24d53b9b50da2a4e82ea22017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05291-3https://doaj.org/toc/2045-2322Abstract Azadirachta indica A. Juss, commonly known as Neem, is the reservoir of triterpenoids of economic importance. Metabolite analysis of different developmental stages of leaf and fruit suggests tissue-specific accumulation of the major triterpenoids in this important tree. Though biosynthesis of these complex molecules requires substrate flux from the isoprenoid pathway, enzymes involved in late biosynthetic steps remain uncharacterized. We established and analyzed transcriptome datasets from leaf and fruit and identified members of gene families involved in intermediate steps of terpenoid backbone biosynthesis and those related to secondary transformation leading to the tissue-specific triterpenoid biosynthesis. Expression analysis suggests differential expression of number of genes between leaf and fruit and probable participation in the biosynthesis of fruit-specific triterpenoids. Genome-wide analysis also identified members of gene families putatively involved in secondary modifications in late biosynthetic steps leading to the synthesis of highly oxygenated triterpenoids. Expression and molecular docking analyses suggest involvement of specific members of CYP450 family in secondary modifications for the biosynthesis of bioactive triterpenoids. This study generated rich genomic resource and identified genes involved in biosynthesis of important molecules, which will aid in the advancement of tools for functional genomics and elucidation of the biosynthesis of triterpenoid from this important tree.Sweta BhambhaniDeepika LakhwaniParul GuptaAshutosh PandeyYogeshwar Vikram DharSumit Kumar BagMehar Hasan AsifPrabodh Kumar TrivediNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sweta Bhambhani Deepika Lakhwani Parul Gupta Ashutosh Pandey Yogeshwar Vikram Dhar Sumit Kumar Bag Mehar Hasan Asif Prabodh Kumar Trivedi Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids
description	Abstract Azadirachta indica A. Juss, commonly known as Neem, is the reservoir of triterpenoids of economic importance. Metabolite analysis of different developmental stages of leaf and fruit suggests tissue-specific accumulation of the major triterpenoids in this important tree. Though biosynthesis of these complex molecules requires substrate flux from the isoprenoid pathway, enzymes involved in late biosynthetic steps remain uncharacterized. We established and analyzed transcriptome datasets from leaf and fruit and identified members of gene families involved in intermediate steps of terpenoid backbone biosynthesis and those related to secondary transformation leading to the tissue-specific triterpenoid biosynthesis. Expression analysis suggests differential expression of number of genes between leaf and fruit and probable participation in the biosynthesis of fruit-specific triterpenoids. Genome-wide analysis also identified members of gene families putatively involved in secondary modifications in late biosynthetic steps leading to the synthesis of highly oxygenated triterpenoids. Expression and molecular docking analyses suggest involvement of specific members of CYP450 family in secondary modifications for the biosynthesis of bioactive triterpenoids. This study generated rich genomic resource and identified genes involved in biosynthesis of important molecules, which will aid in the advancement of tools for functional genomics and elucidation of the biosynthesis of triterpenoid from this important tree.
format	article
author	Sweta Bhambhani Deepika Lakhwani Parul Gupta Ashutosh Pandey Yogeshwar Vikram Dhar Sumit Kumar Bag Mehar Hasan Asif Prabodh Kumar Trivedi
author_facet	Sweta Bhambhani Deepika Lakhwani Parul Gupta Ashutosh Pandey Yogeshwar Vikram Dhar Sumit Kumar Bag Mehar Hasan Asif Prabodh Kumar Trivedi
author_sort	Sweta Bhambhani
title	Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids
title_short	Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids
title_full	Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids
title_fullStr	Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids
title_full_unstemmed	Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids
title_sort	transcriptome and metabolite analyses in azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ddbe79eb7bd24d53b9b50da2a4e82ea2
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Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids

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