Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines

Abstract Endometriosis is a chronic inflammatory disease which increasingly affects young women under 35 years of age and leads to subfertility even infertility. Analysis of the cytotoxic effect of zinc(II) niflumato complex with neocuproine ([Zn(neo)(nif)2] or Zn-Nif) on immortalized human endometr...

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Autores principales:	Miroslava Rabajdová, Ivana Špaková, Zuzana Klepcová, Lukáš Smolko, Michaela Abrahamovská, Peter Urdzík, Mária Mareková
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ddc14f71d5fc4288a7f5bc1a69c646e6
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spelling	oai:doaj.org-article:ddc14f71d5fc4288a7f5bc1a69c646e62021-12-02T17:17:39ZZinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines10.1038/s41598-021-98512-92045-2322https://doaj.org/article/ddc14f71d5fc4288a7f5bc1a69c646e62021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98512-9https://doaj.org/toc/2045-2322Abstract Endometriosis is a chronic inflammatory disease which increasingly affects young women under 35 years of age and leads to subfertility even infertility. Analysis of the cytotoxic effect of zinc(II) niflumato complex with neocuproine ([Zn(neo)(nif)2] or Zn-Nif) on immortalized human endometriotic cell line (12Z) and on control immortalized human endometrial stromal cell line (hTERT) was performed using xCELLigence technology for approximately 72 h following the treatment with Zn-Nif as well as cell viability Trypan Blue Assay. 12Z cell line proliferated more slowly compared to unaffected cells, whereas hTERT cells did not show similar behavior after treatment. The complex probably reduces the effect of pro-inflammatory pathways due to the effect of NSAID, while presence of zinc might reduce the level of ROS and regulate ER2 levels and MMP activity. The observed effects and high selectivity for rapidly proliferating cells with increased inflammatory activity suggest a good prognosis of successful decrease of endometriosis stage with this complex.Miroslava RabajdováIvana ŠpakováZuzana KlepcováLukáš SmolkoMichaela AbrahamovskáPeter UrdzíkMária MarekováNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miroslava Rabajdová Ivana Špaková Zuzana Klepcová Lukáš Smolko Michaela Abrahamovská Peter Urdzík Mária Mareková Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
description	Abstract Endometriosis is a chronic inflammatory disease which increasingly affects young women under 35 years of age and leads to subfertility even infertility. Analysis of the cytotoxic effect of zinc(II) niflumato complex with neocuproine ([Zn(neo)(nif)2] or Zn-Nif) on immortalized human endometriotic cell line (12Z) and on control immortalized human endometrial stromal cell line (hTERT) was performed using xCELLigence technology for approximately 72 h following the treatment with Zn-Nif as well as cell viability Trypan Blue Assay. 12Z cell line proliferated more slowly compared to unaffected cells, whereas hTERT cells did not show similar behavior after treatment. The complex probably reduces the effect of pro-inflammatory pathways due to the effect of NSAID, while presence of zinc might reduce the level of ROS and regulate ER2 levels and MMP activity. The observed effects and high selectivity for rapidly proliferating cells with increased inflammatory activity suggest a good prognosis of successful decrease of endometriosis stage with this complex.
format	article
author	Miroslava Rabajdová Ivana Špaková Zuzana Klepcová Lukáš Smolko Michaela Abrahamovská Peter Urdzík Mária Mareková
author_facet	Miroslava Rabajdová Ivana Špaková Zuzana Klepcová Lukáš Smolko Michaela Abrahamovská Peter Urdzík Mária Mareková
author_sort	Miroslava Rabajdová
title	Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
title_short	Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
title_full	Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
title_fullStr	Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
title_full_unstemmed	Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
title_sort	zinc(ii) niflumato complex effects on mmp activity and gene expression in human endometrial cell lines
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ddc14f71d5fc4288a7f5bc1a69c646e6
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Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines

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