Anti-Toxoplasma Effect of Hydroalchohlic Extract of Terminalia chebula Retz in Cell Culture and Murine Model

Background: We examined anti-Toxoplasma effect of hydroalcoholic extract of Terminalia chebula Retz (T. chebula) in cell culture and murine model. Methods: The study was conducted in Shahrekord University of Medical Sciences, Iran in 2017. Half maximal effective (concentration (EC50) of T. chebul...

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Autores principales:	Mahnaz Jafari, Zahra Lorigooini, Soleiman Kheiri, Kourosh Manouchehri Naeini
Formato:	article
Lenguaje:	EN
Publicado:	Tehran University of Medical Sciences 2021
Materias:	toxoplasma gondii terminalia chebula retz murine tetrazolium salts (mtt) hela cells Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/ddcf1bcbf8c849afa5ad8fbc2a006e02
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spelling	oai:doaj.org-article:ddcf1bcbf8c849afa5ad8fbc2a006e022021-11-29T07:18:54ZAnti-Toxoplasma Effect of Hydroalchohlic Extract of Terminalia chebula Retz in Cell Culture and Murine Model1735-70202008-238Xhttps://doaj.org/article/ddcf1bcbf8c849afa5ad8fbc2a006e022021-11-01T00:00:00Zhttps://ijpa.tums.ac.ir/index.php/ijpa/article/view/3032https://doaj.org/toc/1735-7020https://doaj.org/toc/2008-238XBackground: We examined anti-Toxoplasma effect of hydroalcoholic extract of Terminalia chebula Retz (T. chebula) in cell culture and murine model. Methods: The study was conducted in Shahrekord University of Medical Sciences, Iran in 2017. Half maximal effective (concentration (EC50) of T. chebula extract and pyrimethamine was determined in infected Hela cells by using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) method. In the animal model, BALB/c mice were injected with tachyzoites (104) of T. RH strain intraperitoneally. 24h after the injection, the test groups were orally treated with 100, 200, 400 and 800 mg/kg of T. chebula extract for 7 days. The survival rate of the mice was determined and blood samples were collected to determine the amount of serum Malondialdehyde (MDA) and antioxidant capacity. Then peritoneal fluid of the mice was collected to count the number of tachyzoites and after necropsy, the pathologic changes, including the weight of liver, spleen and kidneys were investigated. The analysis of data was accomplished using SPSS. Results: EC50 values were 94.7μg/mL and 290.50μg/mL for T. chebula and pyrimethamine respectively. In the animal model, the extract of T. chebula in concentration of 100 mg/kg showed the same anti-Toxoplasma effect as pyrimethamine. This concentration of the extract decreased number of intraperitoneal tachyzoites and increased the survival rate of the mice. This extract reduced the levels of serum MDA and tissue inflammation and increased serum antioxidant capacity. Conclusion: Regarding the positive effect of extract, after more clinical trials in the animal model and standardization of the extract, it can be used as an alternative or complementary therapy for toxoplasmosis.Mahnaz JafariZahra LorigooiniSoleiman KheiriKourosh Manouchehri NaeiniTehran University of Medical Sciencesarticletoxoplasma gondiiterminalia chebula retzmurinetetrazolium salts (mtt)hela cellsInfectious and parasitic diseasesRC109-216ENIranian Journal of Parasitology, Vol 16, Iss 4, Pp 631-640 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	toxoplasma gondii terminalia chebula retz murine tetrazolium salts (mtt) hela cells Infectious and parasitic diseases RC109-216
spellingShingle	toxoplasma gondii terminalia chebula retz murine tetrazolium salts (mtt) hela cells Infectious and parasitic diseases RC109-216 Mahnaz Jafari Zahra Lorigooini Soleiman Kheiri Kourosh Manouchehri Naeini Anti-Toxoplasma Effect of Hydroalchohlic Extract of Terminalia chebula Retz in Cell Culture and Murine Model
description	Background: We examined anti-Toxoplasma effect of hydroalcoholic extract of Terminalia chebula Retz (T. chebula) in cell culture and murine model. Methods: The study was conducted in Shahrekord University of Medical Sciences, Iran in 2017. Half maximal effective (concentration (EC50) of T. chebula extract and pyrimethamine was determined in infected Hela cells by using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) method. In the animal model, BALB/c mice were injected with tachyzoites (104) of T. RH strain intraperitoneally. 24h after the injection, the test groups were orally treated with 100, 200, 400 and 800 mg/kg of T. chebula extract for 7 days. The survival rate of the mice was determined and blood samples were collected to determine the amount of serum Malondialdehyde (MDA) and antioxidant capacity. Then peritoneal fluid of the mice was collected to count the number of tachyzoites and after necropsy, the pathologic changes, including the weight of liver, spleen and kidneys were investigated. The analysis of data was accomplished using SPSS. Results: EC50 values were 94.7μg/mL and 290.50μg/mL for T. chebula and pyrimethamine respectively. In the animal model, the extract of T. chebula in concentration of 100 mg/kg showed the same anti-Toxoplasma effect as pyrimethamine. This concentration of the extract decreased number of intraperitoneal tachyzoites and increased the survival rate of the mice. This extract reduced the levels of serum MDA and tissue inflammation and increased serum antioxidant capacity. Conclusion: Regarding the positive effect of extract, after more clinical trials in the animal model and standardization of the extract, it can be used as an alternative or complementary therapy for toxoplasmosis.
format	article
author	Mahnaz Jafari Zahra Lorigooini Soleiman Kheiri Kourosh Manouchehri Naeini
author_facet	Mahnaz Jafari Zahra Lorigooini Soleiman Kheiri Kourosh Manouchehri Naeini
author_sort	Mahnaz Jafari
title	Anti-Toxoplasma Effect of Hydroalchohlic Extract of Terminalia chebula Retz in Cell Culture and Murine Model
title_short	Anti-Toxoplasma Effect of Hydroalchohlic Extract of Terminalia chebula Retz in Cell Culture and Murine Model
title_full	Anti-Toxoplasma Effect of Hydroalchohlic Extract of Terminalia chebula Retz in Cell Culture and Murine Model
title_fullStr	Anti-Toxoplasma Effect of Hydroalchohlic Extract of Terminalia chebula Retz in Cell Culture and Murine Model
title_full_unstemmed	Anti-Toxoplasma Effect of Hydroalchohlic Extract of Terminalia chebula Retz in Cell Culture and Murine Model
title_sort	anti-toxoplasma effect of hydroalchohlic extract of terminalia chebula retz in cell culture and murine model
publisher	Tehran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/ddcf1bcbf8c849afa5ad8fbc2a006e02
work_keys_str_mv	AT mahnazjafari antitoxoplasmaeffectofhydroalchohlicextractofterminaliachebularetzincellcultureandmurinemodel AT zahralorigooini antitoxoplasmaeffectofhydroalchohlicextractofterminaliachebularetzincellcultureandmurinemodel AT soleimankheiri antitoxoplasmaeffectofhydroalchohlicextractofterminaliachebularetzincellcultureandmurinemodel AT kouroshmanouchehrinaeini antitoxoplasmaeffectofhydroalchohlicextractofterminaliachebularetzincellcultureandmurinemodel
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Anti-Toxoplasma Effect of Hydroalchohlic Extract of Terminalia chebula Retz in Cell Culture and Murine Model

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