Bioprospection of Basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence

Abstract Macrofungi belonging to the phylum Basidiomycota are mostly used as medicinal mushrooms in many countries. In the present study, hundred basidiocarp of macrofungi were collected from Tamilnadu during rainy season. The basidiocarp was found in association with root/trunk of living trees, woo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Thangamalai Mowna Sundari, A. Alwin Prem Anand, Packiaraj Jenifer, Rajaiah Shenbagarathai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ddd7066bdf134422b98aa758283d61c1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ddd7066bdf134422b98aa758283d61c1
record_format	dspace
spelling	oai:doaj.org-article:ddd7066bdf134422b98aa758283d61c12021-12-02T12:32:35ZBioprospection of Basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence10.1038/s41598-018-29046-w2045-2322https://doaj.org/article/ddd7066bdf134422b98aa758283d61c12018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29046-whttps://doaj.org/toc/2045-2322Abstract Macrofungi belonging to the phylum Basidiomycota are mostly used as medicinal mushrooms in many countries. In the present study, hundred basidiocarp of macrofungi were collected from Tamilnadu during rainy season. The basidiocarp was found in association with root/trunk of living trees, wood log and decayed matter. Among the hundred basidiocarp, 49 were grown into axenic cultures. Notable variations in the macroscopic characteristics of the basidiome and culture morphology were observed. To study the genetic diversity, the molecular taxonomy of the isolates was carried out using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence marker. Thirty-two strains belonging to the order Polyporales, Hymenochataeles and Russuales under the division Basidiomycota were classified based on phylogeny analysis. This study provides first evidence for the occurrence of species Fulvifomes fastuosus (LDCMY39 and LDCMY43) and Ganoderma wiiroense (LDCMY02, LDCMY08, LDCMY11, LDCMY17 and LDCMY19) from southern India. Molecular evidence for the existence of Phellinus badius was given for the first time as well. These data enhance our understanding on the diversity of macrofungi in India, which could be further exploited for biomedical applications.Thangamalai Mowna SundariA. Alwin Prem AnandPackiaraj JeniferRajaiah ShenbagarathaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-18 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thangamalai Mowna Sundari A. Alwin Prem Anand Packiaraj Jenifer Rajaiah Shenbagarathai Bioprospection of Basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence
description	Abstract Macrofungi belonging to the phylum Basidiomycota are mostly used as medicinal mushrooms in many countries. In the present study, hundred basidiocarp of macrofungi were collected from Tamilnadu during rainy season. The basidiocarp was found in association with root/trunk of living trees, wood log and decayed matter. Among the hundred basidiocarp, 49 were grown into axenic cultures. Notable variations in the macroscopic characteristics of the basidiome and culture morphology were observed. To study the genetic diversity, the molecular taxonomy of the isolates was carried out using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence marker. Thirty-two strains belonging to the order Polyporales, Hymenochataeles and Russuales under the division Basidiomycota were classified based on phylogeny analysis. This study provides first evidence for the occurrence of species Fulvifomes fastuosus (LDCMY39 and LDCMY43) and Ganoderma wiiroense (LDCMY02, LDCMY08, LDCMY11, LDCMY17 and LDCMY19) from southern India. Molecular evidence for the existence of Phellinus badius was given for the first time as well. These data enhance our understanding on the diversity of macrofungi in India, which could be further exploited for biomedical applications.
format	article
author	Thangamalai Mowna Sundari A. Alwin Prem Anand Packiaraj Jenifer Rajaiah Shenbagarathai
author_facet	Thangamalai Mowna Sundari A. Alwin Prem Anand Packiaraj Jenifer Rajaiah Shenbagarathai
author_sort	Thangamalai Mowna Sundari
title	Bioprospection of Basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence
title_short	Bioprospection of Basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence
title_full	Bioprospection of Basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence
title_fullStr	Bioprospection of Basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence
title_full_unstemmed	Bioprospection of Basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence
title_sort	bioprospection of basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (its) and 5.8s rrna gene sequence
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ddd7066bdf134422b98aa758283d61c1
work_keys_str_mv	AT thangamalaimownasundari bioprospectionofbasidiomycetesandmolecularphylogeneticanalysisusinginternaltranscribedspaceritsand58srrnagenesequence AT aalwinpremanand bioprospectionofbasidiomycetesandmolecularphylogeneticanalysisusinginternaltranscribedspaceritsand58srrnagenesequence AT packiarajjenifer bioprospectionofbasidiomycetesandmolecularphylogeneticanalysisusinginternaltranscribedspaceritsand58srrnagenesequence AT rajaiahshenbagarathai bioprospectionofbasidiomycetesandmolecularphylogeneticanalysisusinginternaltranscribedspaceritsand58srrnagenesequence
_version_	1718394067615744000

Bioprospection of Basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence

Ejemplares similares