A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable

Abstract Many species, including most flowering plants, are polyploid, possessing multiple genomes. During polyploidisation, fertility is preserved via the evolution of mechanisms to control the behaviour of these multiple genomes during meiosis. On the polyploidisation of wheat, the major meiotic g...

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Autores principales:	Azahara C. Martín, Abdul Kader Alabdullah, Graham Moore
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dde05370f367443d8a544c26abd54302
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spelling	oai:doaj.org-article:dde05370f367443d8a544c26abd543022021-11-14T12:17:47ZA separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable10.1038/s41598-021-01379-z2045-2322https://doaj.org/article/dde05370f367443d8a544c26abd543022021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01379-zhttps://doaj.org/toc/2045-2322Abstract Many species, including most flowering plants, are polyploid, possessing multiple genomes. During polyploidisation, fertility is preserved via the evolution of mechanisms to control the behaviour of these multiple genomes during meiosis. On the polyploidisation of wheat, the major meiotic gene ZIP4 duplicated and diverged, with the resulting new gene TaZIP4-B2 being inserted into chromosome 5B. Previous studies showed that this TaZIP4-B2 promotes pairing and synapsis between wheat homologous chromosomes, whilst suppressing crossover between related (homoeologous) chromosomes. Moreover, in wheat, the presence of TaZIP4-B2 preserves up to 50% of grain number. The present study exploits a ‘separation-of-function’ wheat Tazip4-B2 mutant named zip4-ph1d, in which the Tazip4-B2 copy still promotes correct pairing and synapsis between homologues (resulting in the same pollen profile and fertility normally found in wild type wheat), but which also allows crossover between the related chromosomes in wheat haploids of this mutant. This suggests an improved utility for the new zip4-ph1d mutant line during wheat breeding, compared to the previously described CRISPR Tazip4-B2 and ph1 mutant lines. The results also reveal that loss of suppression of homoeologous crossover between wheat chromosomes does not in itself reduce wheat fertility when promotion of homologous pairing and synapsis by TaZIP4-B2 is preserved.Azahara C. MartínAbdul Kader AlabdullahGraham MooreNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Azahara C. Martín Abdul Kader Alabdullah Graham Moore A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable
description	Abstract Many species, including most flowering plants, are polyploid, possessing multiple genomes. During polyploidisation, fertility is preserved via the evolution of mechanisms to control the behaviour of these multiple genomes during meiosis. On the polyploidisation of wheat, the major meiotic gene ZIP4 duplicated and diverged, with the resulting new gene TaZIP4-B2 being inserted into chromosome 5B. Previous studies showed that this TaZIP4-B2 promotes pairing and synapsis between wheat homologous chromosomes, whilst suppressing crossover between related (homoeologous) chromosomes. Moreover, in wheat, the presence of TaZIP4-B2 preserves up to 50% of grain number. The present study exploits a ‘separation-of-function’ wheat Tazip4-B2 mutant named zip4-ph1d, in which the Tazip4-B2 copy still promotes correct pairing and synapsis between homologues (resulting in the same pollen profile and fertility normally found in wild type wheat), but which also allows crossover between the related chromosomes in wheat haploids of this mutant. This suggests an improved utility for the new zip4-ph1d mutant line during wheat breeding, compared to the previously described CRISPR Tazip4-B2 and ph1 mutant lines. The results also reveal that loss of suppression of homoeologous crossover between wheat chromosomes does not in itself reduce wheat fertility when promotion of homologous pairing and synapsis by TaZIP4-B2 is preserved.
format	article
author	Azahara C. Martín Abdul Kader Alabdullah Graham Moore
author_facet	Azahara C. Martín Abdul Kader Alabdullah Graham Moore
author_sort	Azahara C. Martín
title	A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable
title_short	A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable
title_full	A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable
title_fullStr	A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable
title_full_unstemmed	A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable
title_sort	separation-of-function zip4 wheat mutant allows crossover between related chromosomes and is meiotically stable
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dde05370f367443d8a544c26abd54302
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A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable

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