The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study
Background: Co-administration of two or several either chemotherapeutic agents or conventional drugs as a combination treatment is the most effective method to increase therapeutic efficiency. Additive or synergistic influence are two mechanisms by which combination therapy causes a rise in optimal...
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Tabriz University of Medical Sciences
2021
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oai:doaj.org-article:dde0705bc4b14d4b882963ec9d3e323b2021-11-29T05:53:09ZThe Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study2383-288610.34172/PS.2021.12https://doaj.org/article/dde0705bc4b14d4b882963ec9d3e323b2021-12-01T00:00:00Zhttps://ps.tbzmed.ac.ir/PDF/ps-27-536.pdfhttps://doaj.org/toc/2383-2886Background: Co-administration of two or several either chemotherapeutic agents or conventional drugs as a combination treatment is the most effective method to increase therapeutic efficiency. Additive or synergistic influence are two mechanisms by which combination therapy causes a rise in optimal cancer therapy compared to a single treatment. Methods: Colorimetric assay was carried out to estimate the cytotoxicity of the combined system, followed by apoptosis assay to calculate the number of apoptotic cells. Both 4′,6-diamidino-2-phenylindole (DAPI) staining and DNA ladder were complemented tests to illuminate morphological changes and DNA fracturing on HeLa cancer cells. Results: We revealed that the combination of berberine (BER) and methotrexate (MTX) could inhibit the growth of HeLa cancer cells noticeably. Nevertheless, single BER and MTX were not as effective as a combined system to reduce cell viability at the same dose. Regarding the apoptosis induction and change in morphology of cancer cells’ nucleus, co-treatment of BERand MTX was more effective. The result was complemented with flow cytometry, DAPI staining and DNA ladder, which showed that BER+MTX depicted more anti-cancer effects. Conclusion: The combination therapy of HeLa cancer cells with BER and MTX showed high inhibition effect compared to other treated groups.Hossein MajidzadehMostafa Araj-KhodaeiMaryam GhaffariAmir JafariDariush ShanehbandiMohammadali TorbatiJafar Ezzati Nazhad DolatabadiTabriz University of Medical Sciencesarticlehela cellsmethotrexateberberineherbal medicinenuclear morphologyPharmacy and materia medicaRS1-441ENPharmaceutical Sciences, Vol 27, Iss 4, Pp 536-542 (2021) |
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hela cells methotrexate berberine herbal medicine nuclear morphology Pharmacy and materia medica RS1-441 |
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hela cells methotrexate berberine herbal medicine nuclear morphology Pharmacy and materia medica RS1-441 Hossein Majidzadeh Mostafa Araj-Khodaei Maryam Ghaffari Amir Jafari Dariush Shanehbandi Mohammadali Torbati Jafar Ezzati Nazhad Dolatabadi The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| description |
Background: Co-administration of two or several either chemotherapeutic agents or conventional drugs as a combination treatment is the most effective method to increase therapeutic efficiency. Additive or synergistic influence are two mechanisms by which combination therapy causes a rise in optimal cancer therapy compared to a single treatment. Methods: Colorimetric assay was carried out to estimate the cytotoxicity of the combined system, followed by apoptosis assay to calculate the number of apoptotic cells. Both 4′,6-diamidino-2-phenylindole (DAPI) staining and DNA ladder were complemented tests to illuminate morphological changes and DNA fracturing on HeLa cancer cells. Results: We revealed that the combination of berberine (BER) and methotrexate (MTX) could inhibit the growth of HeLa cancer cells noticeably. Nevertheless, single BER and MTX were not as effective as a combined system to reduce cell viability at the same dose. Regarding the apoptosis induction and change in morphology of cancer cells’ nucleus, co-treatment of BERand MTX was more effective. The result was complemented with flow cytometry, DAPI staining and DNA ladder, which showed that BER+MTX depicted more anti-cancer effects. Conclusion: The combination therapy of HeLa cancer cells with BER and MTX showed high inhibition effect compared to other treated groups. |
| format |
article |
| author |
Hossein Majidzadeh Mostafa Araj-Khodaei Maryam Ghaffari Amir Jafari Dariush Shanehbandi Mohammadali Torbati Jafar Ezzati Nazhad Dolatabadi |
| author_facet |
Hossein Majidzadeh Mostafa Araj-Khodaei Maryam Ghaffari Amir Jafari Dariush Shanehbandi Mohammadali Torbati Jafar Ezzati Nazhad Dolatabadi |
| author_sort |
Hossein Majidzadeh |
| title |
The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| title_short |
The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| title_full |
The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| title_fullStr |
The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| title_full_unstemmed |
The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| title_sort |
combination of berberine and methotrexate enhances anti-cancer effects in hela cancer cell line: a morphological study |
| publisher |
Tabriz University of Medical Sciences |
| publishDate |
2021 |
| url |
https://doaj.org/article/dde0705bc4b14d4b882963ec9d3e323b |
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