IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS

Abstract. In present study, we investigated the in vitro ability of autologous dendritic cells loaded with tumor lysate antigens to stimulate cytotoxic and secretory activity of effector cells from patients with colorectal cancer. To this purpose, we generated antigen-primed dendritic cells (DS) der...

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Autores principales:	V. V. Kurilin, Yu. N. Khantakova, I. A. Obleukhova, Yu. A. Shevchenko, E. V. Kulikova, V. K. Yakushenko, A. V. Sokolov, S. V. Sennikov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	dendritic cells colorectal cancer cytotoxic activity Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ddeb3fcbef404d60beac8ae6568a4a56
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spelling	oai:doaj.org-article:ddeb3fcbef404d60beac8ae6568a4a562021-11-18T08:03:43ZIN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS1563-06252313-741X10.15789/1563-0625-2013-3-235-246https://doaj.org/article/ddeb3fcbef404d60beac8ae6568a4a562014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/626https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. In present study, we investigated the in vitro ability of autologous dendritic cells loaded with tumor lysate antigens to stimulate cytotoxic and secretory activity of effector cells from patients with colorectal cancer. To this purpose, we generated antigen-primed dendritic cells (DS) derived from adherent fraction of peripheral blood mononuclear cells (PBMSc) obtained from patients with colorectal cancer. The DCs were then cocultured with non-adherent mononuclear cells, either in presence of IL-12 and IL-18, or without cytokines. The modulation efficiency was assessed as cytotoxic activity of mononuclear cells towards autologous tumor cells, production of IFNγ, IL-2, IL-4 and the number of perforin-, granzyme B-containing cells. We have demonstrated the ability of dendritic cells to stimulate the in vitro antitumor cytotoxic and secretory activity of effector cells in culture. A combination of dendritic cells with IL-12 and IL-18 is shown to enhance their effects in the mononuclear cells culture, as evidenced by increased percentage of dead autologous tumor cells, higher numbers of perforin(+), granzyme B(+) lymphocytes, and increased production of IFNγ, IL-2 increased.V. V. KurilinYu. N. KhantakovaI. A. ObleukhovaYu. A. ShevchenkoE. V. KulikovaV. K. YakushenkoA. V. SokolovS. V. SennikovSPb RAACIarticledendritic cellscolorectal cancercytotoxic activityImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 15, Iss 3, Pp 235-246 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	dendritic cells colorectal cancer cytotoxic activity Immunologic diseases. Allergy RC581-607
spellingShingle	dendritic cells colorectal cancer cytotoxic activity Immunologic diseases. Allergy RC581-607 V. V. Kurilin Yu. N. Khantakova I. A. Obleukhova Yu. A. Shevchenko E. V. Kulikova V. K. Yakushenko A. V. Sokolov S. V. Sennikov IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS
description	Abstract. In present study, we investigated the in vitro ability of autologous dendritic cells loaded with tumor lysate antigens to stimulate cytotoxic and secretory activity of effector cells from patients with colorectal cancer. To this purpose, we generated antigen-primed dendritic cells (DS) derived from adherent fraction of peripheral blood mononuclear cells (PBMSc) obtained from patients with colorectal cancer. The DCs were then cocultured with non-adherent mononuclear cells, either in presence of IL-12 and IL-18, or without cytokines. The modulation efficiency was assessed as cytotoxic activity of mononuclear cells towards autologous tumor cells, production of IFNγ, IL-2, IL-4 and the number of perforin-, granzyme B-containing cells. We have demonstrated the ability of dendritic cells to stimulate the in vitro antitumor cytotoxic and secretory activity of effector cells in culture. A combination of dendritic cells with IL-12 and IL-18 is shown to enhance their effects in the mononuclear cells culture, as evidenced by increased percentage of dead autologous tumor cells, higher numbers of perforin(+), granzyme B(+) lymphocytes, and increased production of IFNγ, IL-2 increased.
format	article
author	V. V. Kurilin Yu. N. Khantakova I. A. Obleukhova Yu. A. Shevchenko E. V. Kulikova V. K. Yakushenko A. V. Sokolov S. V. Sennikov
author_facet	V. V. Kurilin Yu. N. Khantakova I. A. Obleukhova Yu. A. Shevchenko E. V. Kulikova V. K. Yakushenko A. V. Sokolov S. V. Sennikov
author_sort	V. V. Kurilin
title	IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS
title_short	IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS
title_full	IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS
title_fullStr	IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS
title_full_unstemmed	IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS
title_sort	in vitro stimulation of antitumor cytotoxic activity of mononuclear cells from colorectal cancer patients
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/ddeb3fcbef404d60beac8ae6568a4a56
work_keys_str_mv	AT vvkurilin invitrostimulationofantitumorcytotoxicactivityofmononuclearcellsfromcolorectalcancerpatients AT yunkhantakova invitrostimulationofantitumorcytotoxicactivityofmononuclearcellsfromcolorectalcancerpatients AT iaobleukhova invitrostimulationofantitumorcytotoxicactivityofmononuclearcellsfromcolorectalcancerpatients AT yuashevchenko invitrostimulationofantitumorcytotoxicactivityofmononuclearcellsfromcolorectalcancerpatients AT evkulikova invitrostimulationofantitumorcytotoxicactivityofmononuclearcellsfromcolorectalcancerpatients AT vkyakushenko invitrostimulationofantitumorcytotoxicactivityofmononuclearcellsfromcolorectalcancerpatients AT avsokolov invitrostimulationofantitumorcytotoxicactivityofmononuclearcellsfromcolorectalcancerpatients AT svsennikov invitrostimulationofantitumorcytotoxicactivityofmononuclearcellsfromcolorectalcancerpatients
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IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS

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