An integrated method for cell isolation and migration on a chip

Abstract Tumour cell migration has an important impact on tumour metastasis. Magnetic manipulation is an ascendant method for guiding and patterning cells. Here, a unique miniaturized microfluidic chip integrating cell isolation and migration assay was designed to isolate and investigate cell migrat...

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Autores principales:	Xiaoqing Lv, Zhaoxin Geng, Zhiyuan Fan, Shicai Wang, WeiHua Pei, Hongda Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ddec32ea4b424a439658d7cce9624cc4
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spelling	oai:doaj.org-article:ddec32ea4b424a439658d7cce9624cc42021-12-02T12:32:03ZAn integrated method for cell isolation and migration on a chip10.1038/s41598-017-08661-z2045-2322https://doaj.org/article/ddec32ea4b424a439658d7cce9624cc42017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08661-zhttps://doaj.org/toc/2045-2322Abstract Tumour cell migration has an important impact on tumour metastasis. Magnetic manipulation is an ascendant method for guiding and patterning cells. Here, a unique miniaturized microfluidic chip integrating cell isolation and migration assay was designed to isolate and investigate cell migration. The chip was fabricated and composed of a magnet adapter, a polytetrafluoroethylene(PDMS) microfluidic chip and six magnetic rings. This device was used to isolate MCF-7 cells from MDA-MB-231-RFP cells and evaluate the effects of TGF-β on MCF-7 cells. First, the two cell types were mixed and incubated with magnetic beads modified with an anti-EpCAM antibody. Then, they were slowly introduced into the chip. MCF-7 cells bond to the magnetic beads in a ring-shaped pattern, while MDA-MB-231-RFP cells were washed away by PBS. Cell viability was examined during culturing in the micro-channel. The effects of TGF-β on MCF-7 cells were evaluated by migration distance and protein expression. The integrated method presented here is novel, low-cost and easy for performing cell isolation and migration assay. The method could be beneficial for developing microfluidic device applications for cancer metastasis research and could provide a new method for biological experimentation.Xiaoqing LvZhaoxin GengZhiyuan FanShicai WangWeiHua PeiHongda ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoqing Lv Zhaoxin Geng Zhiyuan Fan Shicai Wang WeiHua Pei Hongda Chen An integrated method for cell isolation and migration on a chip
description	Abstract Tumour cell migration has an important impact on tumour metastasis. Magnetic manipulation is an ascendant method for guiding and patterning cells. Here, a unique miniaturized microfluidic chip integrating cell isolation and migration assay was designed to isolate and investigate cell migration. The chip was fabricated and composed of a magnet adapter, a polytetrafluoroethylene(PDMS) microfluidic chip and six magnetic rings. This device was used to isolate MCF-7 cells from MDA-MB-231-RFP cells and evaluate the effects of TGF-β on MCF-7 cells. First, the two cell types were mixed and incubated with magnetic beads modified with an anti-EpCAM antibody. Then, they were slowly introduced into the chip. MCF-7 cells bond to the magnetic beads in a ring-shaped pattern, while MDA-MB-231-RFP cells were washed away by PBS. Cell viability was examined during culturing in the micro-channel. The effects of TGF-β on MCF-7 cells were evaluated by migration distance and protein expression. The integrated method presented here is novel, low-cost and easy for performing cell isolation and migration assay. The method could be beneficial for developing microfluidic device applications for cancer metastasis research and could provide a new method for biological experimentation.
format	article
author	Xiaoqing Lv Zhaoxin Geng Zhiyuan Fan Shicai Wang WeiHua Pei Hongda Chen
author_facet	Xiaoqing Lv Zhaoxin Geng Zhiyuan Fan Shicai Wang WeiHua Pei Hongda Chen
author_sort	Xiaoqing Lv
title	An integrated method for cell isolation and migration on a chip
title_short	An integrated method for cell isolation and migration on a chip
title_full	An integrated method for cell isolation and migration on a chip
title_fullStr	An integrated method for cell isolation and migration on a chip
title_full_unstemmed	An integrated method for cell isolation and migration on a chip
title_sort	integrated method for cell isolation and migration on a chip
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ddec32ea4b424a439658d7cce9624cc4
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An integrated method for cell isolation and migration on a chip

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