Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles

Kai-Wen Huang,1 Jen-Jie Chieh,2 Herng-Er Horng,2 Chin-Yih Hong,3 Hong-Chang Yang41Department of Surgery and Hepatitis Research Center, National Taiwan University Hospital, Taipei, 2Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei, 3Graduate Institute of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Huang KW, Chieh JJ, Horng HE, Hong CY, Yang HC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ddeee9054c3147268464ecfe5203bb85
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ddeee9054c3147268464ecfe5203bb85
record_format	dspace
spelling	oai:doaj.org-article:ddeee9054c3147268464ecfe5203bb852021-12-02T00:30:22ZCharacteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles1176-91141178-2013https://doaj.org/article/ddeee9054c3147268464ecfe5203bb852012-06-01T00:00:00Zhttp://www.dovepress.com/characteristics-of-magnetic-labeling-on-liver-tumors-with-anti-alpha-f-a10199https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Kai-Wen Huang,1 Jen-Jie Chieh,2 Herng-Er Horng,2 Chin-Yih Hong,3 Hong-Chang Yang41Department of Surgery and Hepatitis Research Center, National Taiwan University Hospital, Taipei, 2Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei, 3Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, 4Department of Physics, National Taiwan University, Taipei, TaiwanAbstract: For preoperative and intraoperative detection of tumor distribution, numerous multimodal contrast agents, such as magnetic nanoparticles (MNPs) with several examination indicators, are currently in development. However, complex materials, configuration, and cost are required for multimodal contrast agents, accompanied by a high possibility of toxicity and low popularity in clinics. Nevertheless, the magnetic labeling of MNPs using bioprobes should be feasible not only in preoperative magnetic resonance imaging (MRI), but also in intraoperative examination based on other magnetic properties. In this study, anti-alpha-fetoprotein (AFP)-mediated Fe3O4 MNPs, injected into mice with liver tumors, were used to examine the characteristics of magnetic labeling. Using MRI and scanning superconducting-quantum-interference-device biosusceptometry (SSB), based on alternating current (AC) susceptibility, the magnetic labeling occurred significantly on the first day post-injection of anti-AFP magnetic fluid (MF), and then decreased over time. However, for both MF without antibodies and an anti-carcinoembryonic antigen MF, no magnetic labeling occured on the first day of their respective post-injection. The favorable agreement indicates that magnetic labeling possesses two magnetic characteristics: distortion of the imaging field and AC susceptibility. In addition, the results of the biopsy tests, anti-AFP staining, and Prussian blue staining show the same dynamics as those of magnetic methodologies and prove that bound MNPs on tumor tissue are rotatable by an AC magnetic field to express AC susceptibility. Therefore, with the simple configuration of antibody-mediated MNPs, magnetic labeling is also feasible for intraoperative examinations using SSB with high mobility and sensitivity.Keywords: alpha-fetoprotein, magnetic resonance imaging, scanning SQUID biosusceptometryHuang KWChieh JJHorng HEHong CYYang HCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2987-2996 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Huang KW Chieh JJ Horng HE Hong CY Yang HC Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles
description	Kai-Wen Huang,1 Jen-Jie Chieh,2 Herng-Er Horng,2 Chin-Yih Hong,3 Hong-Chang Yang41Department of Surgery and Hepatitis Research Center, National Taiwan University Hospital, Taipei, 2Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei, 3Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, 4Department of Physics, National Taiwan University, Taipei, TaiwanAbstract: For preoperative and intraoperative detection of tumor distribution, numerous multimodal contrast agents, such as magnetic nanoparticles (MNPs) with several examination indicators, are currently in development. However, complex materials, configuration, and cost are required for multimodal contrast agents, accompanied by a high possibility of toxicity and low popularity in clinics. Nevertheless, the magnetic labeling of MNPs using bioprobes should be feasible not only in preoperative magnetic resonance imaging (MRI), but also in intraoperative examination based on other magnetic properties. In this study, anti-alpha-fetoprotein (AFP)-mediated Fe3O4 MNPs, injected into mice with liver tumors, were used to examine the characteristics of magnetic labeling. Using MRI and scanning superconducting-quantum-interference-device biosusceptometry (SSB), based on alternating current (AC) susceptibility, the magnetic labeling occurred significantly on the first day post-injection of anti-AFP magnetic fluid (MF), and then decreased over time. However, for both MF without antibodies and an anti-carcinoembryonic antigen MF, no magnetic labeling occured on the first day of their respective post-injection. The favorable agreement indicates that magnetic labeling possesses two magnetic characteristics: distortion of the imaging field and AC susceptibility. In addition, the results of the biopsy tests, anti-AFP staining, and Prussian blue staining show the same dynamics as those of magnetic methodologies and prove that bound MNPs on tumor tissue are rotatable by an AC magnetic field to express AC susceptibility. Therefore, with the simple configuration of antibody-mediated MNPs, magnetic labeling is also feasible for intraoperative examinations using SSB with high mobility and sensitivity.Keywords: alpha-fetoprotein, magnetic resonance imaging, scanning SQUID biosusceptometry
format	article
author	Huang KW Chieh JJ Horng HE Hong CY Yang HC
author_facet	Huang KW Chieh JJ Horng HE Hong CY Yang HC
author_sort	Huang KW
title	Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles
title_short	Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles
title_full	Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles
title_fullStr	Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles
title_full_unstemmed	Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles
title_sort	characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated fe3o4 magnetic nanoparticles
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/ddeee9054c3147268464ecfe5203bb85
work_keys_str_mv	AT huangkw characteristicsofmagneticlabelingonlivertumorswithantialphafetoproteinmediatedfe3o4magneticnanoparticles AT chiehjj characteristicsofmagneticlabelingonlivertumorswithantialphafetoproteinmediatedfe3o4magneticnanoparticles AT hornghe characteristicsofmagneticlabelingonlivertumorswithantialphafetoproteinmediatedfe3o4magneticnanoparticles AT hongcy characteristicsofmagneticlabelingonlivertumorswithantialphafetoproteinmediatedfe3o4magneticnanoparticles AT yanghc characteristicsofmagneticlabelingonlivertumorswithantialphafetoproteinmediatedfe3o4magneticnanoparticles
_version_	1718403712445054976

Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles

Ejemplares similares