Substantial histone reduction modulates genomewide nucleosomal occupancy and global transcriptional output.
The basic unit of genome packaging is the nucleosome, and nucleosomes have long been proposed to restrict DNA accessibility both to damage and to transcription. Nucleosome number in cells was considered fixed, but recently aging yeast and mammalian cells were shown to contain fewer nucleosomes. We s...
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2011
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oai:doaj.org-article:ddef3524839b4c52ab59154434198fc12021-11-18T05:36:07ZSubstantial histone reduction modulates genomewide nucleosomal occupancy and global transcriptional output.1544-91731545-788510.1371/journal.pbio.1001086https://doaj.org/article/ddef3524839b4c52ab59154434198fc12011-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21738444/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The basic unit of genome packaging is the nucleosome, and nucleosomes have long been proposed to restrict DNA accessibility both to damage and to transcription. Nucleosome number in cells was considered fixed, but recently aging yeast and mammalian cells were shown to contain fewer nucleosomes. We show here that mammalian cells lacking High Mobility Group Box 1 protein (HMGB1) contain a reduced amount of core, linker, and variant histones, and a correspondingly reduced number of nucleosomes, possibly because HMGB1 facilitates nucleosome assembly. Yeast nhp6 mutants lacking Nhp6a and -b proteins, which are related to HMGB1, also have a reduced amount of histones and fewer nucleosomes. Nucleosome limitation in both mammalian and yeast cells increases the sensitivity of DNA to damage, increases transcription globally, and affects the relative expression of about 10% of genes. In yeast nhp6 cells the loss of more than one nucleosome in four does not affect the location of nucleosomes and their spacing, but nucleosomal occupancy. The decrease in nucleosomal occupancy is non-uniform and can be modelled assuming that different nucleosomal sites compete for available histones. Sites with a high propensity to occupation are almost always packaged into nucleosomes both in wild type and nucleosome-depleted cells; nucleosomes on sites with low propensity to occupation are disproportionately lost in nucleosome-depleted cells. We suggest that variation in nucleosome number, by affecting nucleosomal occupancy both genomewide and gene-specifically, constitutes a novel layer of epigenetic regulation.Barbara CelonaAssaf WeinerFrancesca Di FeliceFrancesco M MancusoElisa CesariniRiccardo L RossiLorna GregoryDilair BabanGrazisa RossettiPaolo GriantiMassimiliano PaganiTiziana BonaldiJiannis RagoussisNir FriedmanGiorgio CamilloniMarco E BianchiAlessandra AgrestiPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 9, Iss 6, p e1001086 (2011) |
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Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 Barbara Celona Assaf Weiner Francesca Di Felice Francesco M Mancuso Elisa Cesarini Riccardo L Rossi Lorna Gregory Dilair Baban Grazisa Rossetti Paolo Grianti Massimiliano Pagani Tiziana Bonaldi Jiannis Ragoussis Nir Friedman Giorgio Camilloni Marco E Bianchi Alessandra Agresti Substantial histone reduction modulates genomewide nucleosomal occupancy and global transcriptional output. |
| description |
The basic unit of genome packaging is the nucleosome, and nucleosomes have long been proposed to restrict DNA accessibility both to damage and to transcription. Nucleosome number in cells was considered fixed, but recently aging yeast and mammalian cells were shown to contain fewer nucleosomes. We show here that mammalian cells lacking High Mobility Group Box 1 protein (HMGB1) contain a reduced amount of core, linker, and variant histones, and a correspondingly reduced number of nucleosomes, possibly because HMGB1 facilitates nucleosome assembly. Yeast nhp6 mutants lacking Nhp6a and -b proteins, which are related to HMGB1, also have a reduced amount of histones and fewer nucleosomes. Nucleosome limitation in both mammalian and yeast cells increases the sensitivity of DNA to damage, increases transcription globally, and affects the relative expression of about 10% of genes. In yeast nhp6 cells the loss of more than one nucleosome in four does not affect the location of nucleosomes and their spacing, but nucleosomal occupancy. The decrease in nucleosomal occupancy is non-uniform and can be modelled assuming that different nucleosomal sites compete for available histones. Sites with a high propensity to occupation are almost always packaged into nucleosomes both in wild type and nucleosome-depleted cells; nucleosomes on sites with low propensity to occupation are disproportionately lost in nucleosome-depleted cells. We suggest that variation in nucleosome number, by affecting nucleosomal occupancy both genomewide and gene-specifically, constitutes a novel layer of epigenetic regulation. |
| format |
article |
| author |
Barbara Celona Assaf Weiner Francesca Di Felice Francesco M Mancuso Elisa Cesarini Riccardo L Rossi Lorna Gregory Dilair Baban Grazisa Rossetti Paolo Grianti Massimiliano Pagani Tiziana Bonaldi Jiannis Ragoussis Nir Friedman Giorgio Camilloni Marco E Bianchi Alessandra Agresti |
| author_facet |
Barbara Celona Assaf Weiner Francesca Di Felice Francesco M Mancuso Elisa Cesarini Riccardo L Rossi Lorna Gregory Dilair Baban Grazisa Rossetti Paolo Grianti Massimiliano Pagani Tiziana Bonaldi Jiannis Ragoussis Nir Friedman Giorgio Camilloni Marco E Bianchi Alessandra Agresti |
| author_sort |
Barbara Celona |
| title |
Substantial histone reduction modulates genomewide nucleosomal occupancy and global transcriptional output. |
| title_short |
Substantial histone reduction modulates genomewide nucleosomal occupancy and global transcriptional output. |
| title_full |
Substantial histone reduction modulates genomewide nucleosomal occupancy and global transcriptional output. |
| title_fullStr |
Substantial histone reduction modulates genomewide nucleosomal occupancy and global transcriptional output. |
| title_full_unstemmed |
Substantial histone reduction modulates genomewide nucleosomal occupancy and global transcriptional output. |
| title_sort |
substantial histone reduction modulates genomewide nucleosomal occupancy and global transcriptional output. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/ddef3524839b4c52ab59154434198fc1 |
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