Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets

Noormohammadi Z, Taban M, Farahani F. 2018. Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets. Biodiversitas 19: 1805-1810. Catharanthus roseus L.G. Don, is the essential medicinal plant with considerable attention. This p...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	ZAHRA NOORMOHAMMADI, MARYAM TABAN, FARAH FARAHANI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	gene expression periwinkle radiation secondary metabolism tissue culture Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ddf0cdcf7f4e4609aec83952a571ba07
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ddf0cdcf7f4e4609aec83952a571ba07
record_format	dspace
spelling	oai:doaj.org-article:ddf0cdcf7f4e4609aec83952a571ba072021-11-16T14:04:05ZShort Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets1412-033X2085-472210.13057/biodiv/d190530https://doaj.org/article/ddf0cdcf7f4e4609aec83952a571ba072018-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2950https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Noormohammadi Z, Taban M, Farahani F. 2018. Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets. Biodiversitas 19: 1805-1810. Catharanthus roseus L.G. Don, is the essential medicinal plant with considerable attention. This plant is a rich source of terpenoid indole alkaloids (TIAs). The main alkaloids in C. roseus are vinblastine, vincristine, and ajmalicine. The tryptophan decarboxylase (Tdc) and Strictosidine synthase (Str) are key enzymes in TIA biosynthesis. In the present study, Tdc and Str gene expressions, as well as vinblastine production were evaluated in tissue culture regenerated plantlets in 4 groups; control, 60 Gy irradiation, 50 mg/L putrescine and 60 Gy irradiation + 50mg/L putrescine treatments. The results revealed significant increase in Tdc and Str gene expressions in 60 Gy irradiation + 50mg/L putrescine treated plantlets in comparison with control samples by using qPCR methods. HPLC analysis showed a higher amount of vinblastine in 60 Gy + 59 mg/L putrescine treated plantlets. Gamma radiation and putrescine as elicitor and polyamine, respectively, are able to improve vinblastine production in C. roseus.ZAHRA NOORMOHAMMADIMARYAM TABANFARAH FARAHANIMBI & UNS Soloarticlegene expressionperiwinkleradiationsecondary metabolismtissue cultureBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 5, Pp 1805-1810 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	gene expression periwinkle radiation secondary metabolism tissue culture Biology (General) QH301-705.5
spellingShingle	gene expression periwinkle radiation secondary metabolism tissue culture Biology (General) QH301-705.5 ZAHRA NOORMOHAMMADI MARYAM TABAN FARAH FARAHANI Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets
description	Noormohammadi Z, Taban M, Farahani F. 2018. Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets. Biodiversitas 19: 1805-1810. Catharanthus roseus L.G. Don, is the essential medicinal plant with considerable attention. This plant is a rich source of terpenoid indole alkaloids (TIAs). The main alkaloids in C. roseus are vinblastine, vincristine, and ajmalicine. The tryptophan decarboxylase (Tdc) and Strictosidine synthase (Str) are key enzymes in TIA biosynthesis. In the present study, Tdc and Str gene expressions, as well as vinblastine production were evaluated in tissue culture regenerated plantlets in 4 groups; control, 60 Gy irradiation, 50 mg/L putrescine and 60 Gy irradiation + 50mg/L putrescine treatments. The results revealed significant increase in Tdc and Str gene expressions in 60 Gy irradiation + 50mg/L putrescine treated plantlets in comparison with control samples by using qPCR methods. HPLC analysis showed a higher amount of vinblastine in 60 Gy + 59 mg/L putrescine treated plantlets. Gamma radiation and putrescine as elicitor and polyamine, respectively, are able to improve vinblastine production in C. roseus.
format	article
author	ZAHRA NOORMOHAMMADI MARYAM TABAN FARAH FARAHANI
author_facet	ZAHRA NOORMOHAMMADI MARYAM TABAN FARAH FARAHANI
author_sort	ZAHRA NOORMOHAMMADI
title	Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets
title_short	Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets
title_full	Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets
title_fullStr	Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets
title_full_unstemmed	Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets
title_sort	short communication: the impact of gamma radiation on tdc and str gene expressions in catharanthus roseus regenerated plantlets
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/ddf0cdcf7f4e4609aec83952a571ba07
work_keys_str_mv	AT zahranoormohammadi shortcommunicationtheimpactofgammaradiationontdcandstrgeneexpressionsincatharanthusroseusregeneratedplantlets AT maryamtaban shortcommunicationtheimpactofgammaradiationontdcandstrgeneexpressionsincatharanthusroseusregeneratedplantlets AT farahfarahani shortcommunicationtheimpactofgammaradiationontdcandstrgeneexpressionsincatharanthusroseusregeneratedplantlets
_version_	1718426391183097856

Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets

Ejemplares similares