CLINICAL IMPORTANCE OF DETERMINATION OF HLA-DRB1 LOCUS GENES IN RHEUMATOID ARTHRITIS

Rheumatoid arthritis (RA) is a most common autoimmune inflammatory arthritis in adults. Serological marker of RA are rheumatoid factor (RF) and antibodies to cyclic citrullinated peptide (ACCP). The main genetic factor that determine predisposition to RA is HLA-DRB1 alleles. The HLA-DRB1 locus allel...

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Autores principales:	V. Guseva, S. Lapin, V. Myachikova, A. Maslyanski, A. Chuchlovin, N. Ivanova, O. Tkachenko, T. Blinova, A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	rheumatoid arthritis antibodies to cyclic citrullinated peptide smoking hla-drb1 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ddf6dedc6e0c4f90a0cfb20b76d9d2d2
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spelling	oai:doaj.org-article:ddf6dedc6e0c4f90a0cfb20b76d9d2d22021-11-18T08:03:48ZCLINICAL IMPORTANCE OF DETERMINATION OF HLA-DRB1 LOCUS GENES IN RHEUMATOID ARTHRITIS1563-06252313-741X10.15789/1563-0625-2019-2-333-340https://doaj.org/article/ddf6dedc6e0c4f90a0cfb20b76d9d2d22019-05-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1500https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XRheumatoid arthritis (RA) is a most common autoimmune inflammatory arthritis in adults. Serological marker of RA are rheumatoid factor (RF) and antibodies to cyclic citrullinated peptide (ACCP). The main genetic factor that determine predisposition to RA is HLA-DRB1 alleles. The HLA-DRB1 locus alleles may encode a common 5-amino acid sequence called ‘shared epitope’ (SE). The aim of our study is to assess the clinical significance and occurrence of SE and HLA-DRB1 genes and to analyze the prognostic significance of these factors for RA patients. We collected a serum and DNA samples from 72 patients with RA. For genotyping of HLA-DRB1 locus “DNA-Technology” kits (Moscow, Russia) were used. HLA-DRB1 SE sequences were genotyped by real-time PCR with specific primers. Determination of ACCP in serum was performed by ELISA (Euroimmun AG, Lübeck, Germany), RF detection, by turbidimetric method. Clinical status of the disease was assessed using the RA DAS-28 Activity Index. We have obtained the following results: determination of HLA-DRB1 gene frequency in the North-West region of Russia showed that the HLA-DRB104 gene variant occurred at 11.4%, HLA-DRB101, 14.2%. HLA-DRB110 and HLA- DRB114 occured, respectively, in 0,8% and 2% of the cases. The DRB104 and DRB101 allelic variants were found in 73.6% of patients with RA, and in 43.9% of the control group. Among patients with RA, the SE gene frequency was 66.6%. SE is associated with ACCP detection and higher DAS28 index. Conclusions: The allelic variations of HLA-DRB encoding SE are associated with ACCP-positive RA in the population of the North-West region of the Russian Federation. Identification of HLA-DRB1 allelic gene variants and SE sequences in this locus serve as an additional test to specify serological diagnosis in rheumatoid arthritis.V. GusevaS. LapinV. MyachikovaA. MaslyanskiA. ChuchlovinN. IvanovaO. TkachenkoT. BlinovaA. TotolianSPb RAACIarticlerheumatoid arthritisantibodies to cyclic citrullinated peptidesmokinghla-drb1Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 2, Pp 333-340 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	rheumatoid arthritis antibodies to cyclic citrullinated peptide smoking hla-drb1 Immunologic diseases. Allergy RC581-607
spellingShingle	rheumatoid arthritis antibodies to cyclic citrullinated peptide smoking hla-drb1 Immunologic diseases. Allergy RC581-607 V. Guseva S. Lapin V. Myachikova A. Maslyanski A. Chuchlovin N. Ivanova O. Tkachenko T. Blinova A. Totolian CLINICAL IMPORTANCE OF DETERMINATION OF HLA-DRB1 LOCUS GENES IN RHEUMATOID ARTHRITIS
description	Rheumatoid arthritis (RA) is a most common autoimmune inflammatory arthritis in adults. Serological marker of RA are rheumatoid factor (RF) and antibodies to cyclic citrullinated peptide (ACCP). The main genetic factor that determine predisposition to RA is HLA-DRB1 alleles. The HLA-DRB1 locus alleles may encode a common 5-amino acid sequence called ‘shared epitope’ (SE). The aim of our study is to assess the clinical significance and occurrence of SE and HLA-DRB1 genes and to analyze the prognostic significance of these factors for RA patients. We collected a serum and DNA samples from 72 patients with RA. For genotyping of HLA-DRB1 locus “DNA-Technology” kits (Moscow, Russia) were used. HLA-DRB1 SE sequences were genotyped by real-time PCR with specific primers. Determination of ACCP in serum was performed by ELISA (Euroimmun AG, Lübeck, Germany), RF detection, by turbidimetric method. Clinical status of the disease was assessed using the RA DAS-28 Activity Index. We have obtained the following results: determination of HLA-DRB1 gene frequency in the North-West region of Russia showed that the HLA-DRB104 gene variant occurred at 11.4%, HLA-DRB101, 14.2%. HLA-DRB110 and HLA- DRB114 occured, respectively, in 0,8% and 2% of the cases. The DRB104 and DRB101 allelic variants were found in 73.6% of patients with RA, and in 43.9% of the control group. Among patients with RA, the SE gene frequency was 66.6%. SE is associated with ACCP detection and higher DAS28 index. Conclusions: The allelic variations of HLA-DRB encoding SE are associated with ACCP-positive RA in the population of the North-West region of the Russian Federation. Identification of HLA-DRB1 allelic gene variants and SE sequences in this locus serve as an additional test to specify serological diagnosis in rheumatoid arthritis.
format	article
author	V. Guseva S. Lapin V. Myachikova A. Maslyanski A. Chuchlovin N. Ivanova O. Tkachenko T. Blinova A. Totolian
author_facet	V. Guseva S. Lapin V. Myachikova A. Maslyanski A. Chuchlovin N. Ivanova O. Tkachenko T. Blinova A. Totolian
author_sort	V. Guseva
title	CLINICAL IMPORTANCE OF DETERMINATION OF HLA-DRB1 LOCUS GENES IN RHEUMATOID ARTHRITIS
title_short	CLINICAL IMPORTANCE OF DETERMINATION OF HLA-DRB1 LOCUS GENES IN RHEUMATOID ARTHRITIS
title_full	CLINICAL IMPORTANCE OF DETERMINATION OF HLA-DRB1 LOCUS GENES IN RHEUMATOID ARTHRITIS
title_fullStr	CLINICAL IMPORTANCE OF DETERMINATION OF HLA-DRB1 LOCUS GENES IN RHEUMATOID ARTHRITIS
title_full_unstemmed	CLINICAL IMPORTANCE OF DETERMINATION OF HLA-DRB1 LOCUS GENES IN RHEUMATOID ARTHRITIS
title_sort	clinical importance of determination of hla-drb1 locus genes in rheumatoid arthritis
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/ddf6dedc6e0c4f90a0cfb20b76d9d2d2
work_keys_str_mv	AT vguseva clinicalimportanceofdeterminationofhladrb1locusgenesinrheumatoidarthritis AT slapin clinicalimportanceofdeterminationofhladrb1locusgenesinrheumatoidarthritis AT vmyachikova clinicalimportanceofdeterminationofhladrb1locusgenesinrheumatoidarthritis AT amaslyanski clinicalimportanceofdeterminationofhladrb1locusgenesinrheumatoidarthritis AT achuchlovin clinicalimportanceofdeterminationofhladrb1locusgenesinrheumatoidarthritis AT nivanova clinicalimportanceofdeterminationofhladrb1locusgenesinrheumatoidarthritis AT otkachenko clinicalimportanceofdeterminationofhladrb1locusgenesinrheumatoidarthritis AT tblinova clinicalimportanceofdeterminationofhladrb1locusgenesinrheumatoidarthritis AT atotolian clinicalimportanceofdeterminationofhladrb1locusgenesinrheumatoidarthritis
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CLINICAL IMPORTANCE OF DETERMINATION OF HLA-DRB1 LOCUS GENES IN RHEUMATOID ARTHRITIS

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